A5100447462- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Korean Urban and Social Studies
- Food Quality and Safety Studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Gene expression and cancer classification
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Bacteriophages and microbial interactions
- Bioinformatics and Genomic Networks
- Listeria monocytogenes in Food Safety
- Healthcare and Venom Research
- Marine and coastal ecosystems
- Polar Research and Ecology
- Microbial Community Ecology and Physiology
- Avian ecology and behavior
- Microbial Metabolism and Applications
- Insect symbiosis and bacterial influences
- Education and Learning Interventions
- Cell Image Analysis Techniques
- Agriculture, Soil, Plant Science
- Coastal wetland ecosystem dynamics
- Salmonella and Campylobacter epidemiology
Chonnam National University
2018-2024
Virginia Tech
2015-2024
Korea Institute of Materials Science
2024
Institute for Basic Science
2024
Sungkyunkwan University
2024
St George's, University of London
2023
International Vaccine Institute
2023
Korea Advanced Institute of Science and Technology
2022
National Institute of Fisheries Science
2022
Heidelberg University
2022
Zr-based MOP cages are cross-linked with flexible organic molecules through a condensation reaction, resulting in highly crystalline framework.
Gut microbiota and the immune system interact to maintain tissue homeostasis, but whether this interaction is involved in pathogenesis of systemic lupus erythematosus (SLE) unclear. Here we report that oral antibiotics given during active disease removed harmful bacteria from gut attenuated SLE-like lupus-prone mice. Using MRL/lpr mice, showed after onset ameliorated autoimmunity kidney histopathology. They decreased IL-17-producing cells increased level circulating IL-10. In addition,...
Abstract Background New sequencing technologies have lowered financial barriers to whole genome sequencing, but resulting assemblies are often fragmented and far from ‘finished’. Updating multi-scaffold drafts chromosome-level status can be achieved through experimental mapping or re-sequencing efforts. Avoiding the costs associated with such approaches, comparative genomic analysis of gene order conservation (synteny) predict scaffold neighbours (adjacencies) offers a potentially useful...
Increasing evidence indicates that many, if not all, small genes encoding proteins ≤100 aa are missing in annotations of bacterial genomes currently available. To uncover unannotated the model bacterium Salmonella enterica Typhimurium 14028s, we used genomic technique ribosome profiling, which provides a snapshot all mRNAs being translated (translatome) given growth condition. For comprehensive identification genes, obtained translatomes from four different conditions: LB, MOPS rich defined...
Recent advances in genomic technologies have generated data on large-scale protein-DNA interactions and open chromatin regions for many eukaryotic species. How to identify condition-specific functions of transcription factors using these has become a major challenge research. To solve this problem, we developed method called ConSReg, which provides novel approach integrate regulatory into predictive machine learning models key genes. Using Arabidopsis as model system, tested our genes sets...
Salmonella is one of the main foodborne pathogens that affect humans and farm animals. The genus comprises a group food-transmitted cause highly prevalent diseases throughout world. aim this study was to appraise viability Typhimurium biofilm under water treatment at room temperature on different surfaces, specifically stainless steel (SS), plastic (PLA), rubber (RB), eggshell (ES). After 35 D, reduction SS, PLA, RB, ES 3.35, 3.57, 3.22, 2.55 log CFU/coupon without 4.31, 4.49, 3.50, 1.49...
An essential step in the analysis of single-cell RNA sequencing data is to classify cells into specific cell types using marker genes. In this study, we have developed a machine learning pipeline called predictive (SPmarker) identify novel cell-type genes Arabidopsis root. Unlike traditional approaches, our method uses interpretable models select We demonstrated that can: assign based on were labelled published methods; project identified by trajectory from one set other sets; and internal...
Specific genomic loci, termed Piwi-interacting RNA (piRNA) clusters, manufacture piRNAs that serve as guides for the inactivation of complementary transposable elements (TEs). The piRNA pathway has been accurately detailed in Drosophila melanogaster, while it remains poorly examined other insects. This is increasingly recognized critical germline development and reproduction. Understanding functions mosquitoes could offer an opportunity disease vector control by reduction their reproductive...
Abstract Background Current Genome-Wide Association Studies (GWAS) are performed in a single trait framework without considering genetic correlations between important disease traits. Hence, the GWAS have limitations discovering risk factors affecting pleiotropic effects. Results This work reports novel data mining approach to discover patterns of multiple phenotypic associations over 52 anthropometric and biochemical traits KARE new analytical scheme for multivariate phenotypes defined by...
Scalable microfluidic devices containing reaction and loading chambers were developed to conduct single-cell transcriptomic studies.
We previously showed that all-trans-retinoic acid (tRA), an active metabolite of vitamin A, exacerbated pre-existing autoimmunity in lupus; however, its effects before the development are unknown. Here, using a pristane-induced model, we show tRA exerts differential when given at initiation vs. continuation phase lupus. Unlike treatment during disease, pre-pristane with aggravated glomerulonephritis through increasing renal expression pro-fibrotic protein laminin β1, activating bone marrow...
Subclinical doses of LPS (SD-LPS) are known to cause low-grade inflammatory activation monocytes, which could lead diseases including atherosclerosis and metabolic syndrome. Sodium 4-phenylbutyrate is a potential therapeutic compound can reduce the inflammation caused by SD-LPS. To understand gene regulatory networks these processes, we have generated scRNA-seq data from mouse monocytes treated with compounds identified 11 novel cell clusters. We developed machine learning method integrate...
민들레 꽃, 잎, 뿌리, 전초의 부위별 항산화 활성과 생리활성을 탐색하기 위해 에탄올 추출물에서 총 플라보노이드와 폴리페놀 함량을 측정하였으며 활성 그리고 tyrosinase 억제 활성을 분석하였다. 플라보노이드 함량은 잎 추출물이 41.66 ㎎/g으로 다른 부위 추출물보다 높았고 꽃 71.91 가장 많이 함유하는 것으로 나타났다. 전자공여능은 1 ㎎/mL의 농도에서 전초 뿌리 각각 92.25%, 88.18%, 84.55%, 83.40%로 높은 나타내었으며 농도의존적으로 활성이 유의하게 증가되었다. SOD 유사활성능은 1.0 8.40～11.20%를 추출물의 pH 1.2 조건에서 측정한 아질산염 소거능은 꽃과 47.37%와 47.18%로 전초와 뿌리보다 높았다. Tyrosinase 저해 활성은 34.22%로 높았으며 추출물에서도 20% 이상의 결과 및 우수한 지녀 이를 기능성 건강식품의 소재로 활용할 수 있다고 사료된다.
Various organizations offer online continuing dental education (CDE) courses. While previous investigations focused on objective measures to determine the quality of courses, this exploratory study evaluates participants' experience with them. We surveyed 436 past course participants from nine CDE courses (courses provided by six organizations) regarding their taken. Our analysis 169 responses (38.8 percent response rate) focuses how can be characterized; whether expectations were met...
By screening a collection of Salmonella mutants deleted for genes encoding small proteins ≤60 amino acids, we identified three paralogous (ymdF, STM14_1829, and yciG) required wild-type flagellum-dependent swimming swarming motility. The ymdF, yciG encode 55, 60, 60 acid residues, respectively. A bioinformatics analysis predicted that these are intrinsically disordered proteins, circular dichroism purified recombinant confirmed all unstructured in solution. mutant STM14_1829 showed the most...
Abstract WDR11 , a gene associated with Kallmann syndrome, is important in reproductive system development but molecular understanding of its action remains incomplete. We previously reported that Wdr11 -deficient embryos exhibit defective ciliogenesis and developmental defects Hedgehog (HH) signalling. Here we demonstrate required for primordial germ cell (PGC) development, regulating canonical noncanonical HH signalling parallel. Loss disrupts PGC motility proliferation driven by the...
SUMMARY Stress-sensitive and stress-adapted plants respond differently to environmental stresses. To explore the cellular-level stress adaptations, we built root single-cell transcriptome atlases for diverse Brassicaceae species: stress-sensitive ( Arabidopsis thaliana Sisymbrium irio ), extremophytes Eutrema salsugineum Schrenkiella parvula ) a polyploid crop Camelina sativa under control, NaCl, abscisic acid treatments. Approximately half of cell-type markers lacked expression conservation...
A novel approach is introduced using nanoplasmonic microarray–based solid-phase recombinase polymerase amplification (RPA) that offers high sensitivity and multiplexing capabilities for gene detection. Nanoplasmonic microarrays were developed through one-step immobilization of streptavidin/biotin primers fine-tuning the amplicon size to achieve plasmon-enhanced fluorescence (PEF) on substrate, thereby improving sensitivity. The specificity RPA was evaluated in detecting E, N, RdRP genes...
Reversible posttranslational modification of proteins by the action small ubiquitin-like modifier (SUMO) peptide (sumoylation) has been known to participate in various biological processes eukaryotes. However, much less is about role sumoylation plants. In our recent paper which we write this Addendum, show that loss SIZ1, a SUMO E3 ligase, results highly increased SA-mediated defense signaling through PAD4-dependent pathway. This leads constitutively expressed pathogen related (PR) genes...
Comparative transcriptome analysis is the comparison of expression patterns between homologous genes in different species. Since most molecular mechanistic studies plants have been performed model species, including Arabidopsis and rice, comparative particularly important for functional annotation diverse plant Many biological processes, such as embryo development, are highly conserved The challenge to establish one-to-one mapping developmental stages two In this manuscript, we solve problem...
The crude ethanol extract of the leaves Spiraea thunbergii Sieb. (Rosaceae) showed antibacterial activity against a food borne pathogen, Escherichia coli. Bioactivity-guided separation led to isolation butyrolaton, 1(S-(-)-tulipalin B). structure was determined from interpretation spectroscopic data (UV, MS, and NMR). minimal inhibitory concentration (MIC) compound 1 E. coli found be 100/ml. In addition, we possibility that methylene group may operate as key factor in activities and the...