A5039226532- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Aerosol Filtration and Electrostatic Precipitation
- Biosensors and Analytical Detection
- RNA Research and Splicing
- Vehicle emissions and performance
- Air Quality and Health Impacts
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Biotin and Related Studies
- Air Quality Monitoring and Forecasting
- Metal complexes synthesis and properties
- Carbon Dioxide Capture Technologies
- Molecular Junctions and Nanostructures
- Nanofabrication and Lithography Techniques
- DNA Repair Mechanisms
- Cyclone Separators and Fluid Dynamics
- Chemical Synthesis and Analysis
Korea Advanced Institute of Science and Technology
2016-2025
Inha University
2001-2025
Hankuk University of Foreign Studies
2022
Hallym University
2021
Hallym University Medical Center
2021
Hallym University Sacred Heart Hospital
2021
Daejeon University
2015-2019
Government of the Republic of Korea
2014-2019
Kootenay Association for Science & Technology
2014-2019
Korea Research Institute of Bioscience and Biotechnology
2007-2011
Antibody immobilization on a solid support is an essential process for the development of most immune-based assay systems. The choice method greatly affects antibody–antigen interactions surface. For past several years, numerous strategies have been reported to control antibody immobilization, mainly by directing orientation, stability, and density bound antibodies different platforms. Here we discuss recent developments in methods with particular focus strengths limitations approaches,...
Protein G is an antibody binding protein, which specifically targets the Fc region of antibody. It therefore has been widely used to immobilize different types antibodies in numerous immunoassays. Here, we have engineered Streptococcus protein contain various numbers cysteine residues at N-terminus and form well-oriented films on bare gold. SPR imaging analyses indicated that a gold surface treated with cysteine-tagged possesses superior ability compared one tag-free G. AFM images higher...
Two is better than one: short locked nucleic acid based probes were used to collectively capture and detect microRNAs by a simple two-temperature hybridization process. Intact directly measured down attomolar concentrations with high specificity nearly four orders of magnitude dynamic range. Single base mismatches in the potently discriminated from perfectly matched targets.
A versatile biolinker for efficient antibody immobilization was prepared by site-specific coupling of protein G to DNA oligonucleotide. This G−DNA conjugate ensures the controlled an intended area on surface bioassay chips or particles, while maintaining activity and orientation bound antibody. Streptococcus tagged with a cysteine residue at N-terminus chemically linked amine-modified, single-stranded DNA. SPR analysis indicated that conjugates sequence-specifically bind complementary...
Abstract Human ferritins are emerging platforms for non‐toxic protein‐based drug delivery, owing to their intrinsic or acquirable targeting abilities cancer cells and hollow cage structures loading. However, reliable strategies high‐level encapsulation within ferritin cavities prompt cellular release still lacking. Ferritin nanocages were developed with partially opened hydrophobic channels, which provide stable routes spontaneous highly accumulated loading of Fe II ‐conjugated drugs as well...
Abstract Supramolecular protein assemblies offer novel nanoscale architectures with molecular precision and unparalleled functional diversity. A key challenge, however, is to create precise nano-assemblies of proteins both defined structures a controlled number protein-building blocks. Here we report series supramolecular green fluorescent oligomers that are assembled in polygonal geometries prepared monodisperse population. Green engineered be self-assembled cells into oligomeric natively...
Abstract Phase separation of specific biomolecules into liquid droplet-like condensates is a key mechanism to form membrane-less organelles, which spatio-temporally organize diverse biochemical processes in cells. To investigate the working principles these biomolecular as dynamic reaction centers, precise control condensate properties essential. Here, we design strategy for metal ion-induced clustering minimal protein modules produce condensates, can be widely varied by simple manipulation...
Proactive management of foodborne illness requires routine surveillance pathogens, which developing simple, rapid, and sensitive detection methods. Here, a strategy is presented that enables the multiple bacteria using 3D nanostructure swab deep learning-based Raman signal classification. The efficiently captures portable instrument directly collects signals captured bacteria. learning algorithm has been demonstrated, 1D convolutional neural network with binary labeling, achieves superior...
HMGB1, a highly conserved non-histone DNA-binding protein, interacts with specific DNA structural motifs such as those encountered at cisplatin damage, four-way junctions, and supercoils. The interaction of full-length containing two tandem HMG box domains C-terminal acidic tail, cisplatin-modified was investigated by hydroxyl radical footprinting electrophoretic gel mobility shift assays. HMGB1 protein binds to 1,2-intrastrand d(GpG) cross-link mainly through domain A, revealed...
Here we report new photoactivable antibody binding proteins, which site-selectively capture antibodies and form covalent conjugates with captured upon irradiation. The proteins allow the site-selective tagging and/or immobilization of a highly preferred orientation omit need for prior modifications. minimal Fc-binding domain protein G, widely used protein, was genetically chemically engineered to contain site-specific photo cross-linker, benzophenone. In addition, further mutated have an...
Like an antibody: A novel structure-specific RNA-binding protein was designed to stably and specifically bind surface-bound microRNAs. By acting like antibody, this RNA binder enabled the universal detection of hybridized microRNAs on array surfaces (see picture) without any enzymatic amplification or labeling reactions. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited typeset. They made available...
Supersaturation, nucleation, and phase separation are ubiquitous phenomena of great interest in both science industry. However, a unified, quantitative understanding these has yet to be achieved for mesoscopic materials. Here, we present set general equations that determine the monomer saturation degree, size distribution free energy materials, as well their transition conditions. These reveal that, under supersaturation, largest cluster (LCS) is an important state-variable; supersaturation...
Phase separation of specific proteins into liquid-like condensates is a key mechanism for forming membrane-less organelles, which organize diverse cellular processes in space and time. These protein hold immense potential as biomaterials capable containing sets biomolecules with high densities dynamic liquid properties. Despite their appeal, methods to manipulate condensate materials remain largely unexplored. Here, we present one-pot assembly method assemble coalescence-resistant...
A gas‐liquid Eulerian porous media computational fluid dynamics (CFD) model was developed for an absorber with structured packing to remove CO 2 from natural gas by mono‐ethanol‐amine (MEA). The three‐dimensional geometry of the amine Mellapak 500.X constructed investigate effect tilting and motion experienced on ships barges offshore plants. momentum equation included resistance, exchange, liquid dispersion replace structured‐packing media. mass involved transfer into MEA solution, one...
The consequences of human RNA polymerase II (pol II) arrest at the site DNA damaged by cisplatin were studied in whole cells and cell extracts, with a particular focus on stability stalled pol its subsequent ubiquitylation. Site-specifically platinated templates immobilized solid support used to perform vitro transcription HeLa nuclear extracts. elongation was completely blocked intrastrand cross-link. quite stable, remaining template damage also observed live cells. A fractionation...
Utilizing a gold enhancement process after inducing electrostatic interaction between positively charged nanoparticles and negatively target DNA hybridized to neutral PNA capture probes, new method for label-free detection of was developed successfully applied detect H5-type DNA.
MicroRNAs (miRNAs) are emerging new biomarkers for many human diseases. To fully employ miRNAs as clinical diagnosis, it is most desirable to accurately determine the expression patterns of miRNAs. The optimum miRNA profiling method would feature 1) highest sensitivity with a wide dynamic range accurate patterns, 2) supreme specificity discriminate single nucleotide polymorphisms (SNPs), and 3) simple sensing processes minimize measurement variation. Here, an ultra‐specific detection...
Multivalent interactions between amino acid residues of intrinsically disordered proteins (IDPs) drive phase separation these into liquid condensates, forming various membrane-less organelles in cells. These often biased IDPs are also likely involved selective recruitment many other condensates. However, determining factors for this IDP protein condensates not understood yet. Here, we quantitatively examined tendencies with different sequence compositions IDP-clustered both vitro as well...