A5027030666- Diet and metabolism studies
- Gut microbiota and health
- Retinal Development and Disorders
- Asthma and respiratory diseases
- Gastrointestinal motility and disorders
- Dietary Effects on Health
- Single-cell and spatial transcriptomics
- Wnt/β-catenin signaling in development and cancer
- CRISPR and Genetic Engineering
- FOXO transcription factor regulation
- Crystallization and Solubility Studies
- Ocular Oncology and Treatments
- Pharmacological Effects of Natural Compounds
- Nutrition and Health in Aging
- Cancer Cells and Metastasis
- Sinusitis and nasal conditions
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Virus-based gene therapy research
- Clinical Nutrition and Gastroenterology
- Adenosine and Purinergic Signaling
- Protease and Inhibitor Mechanisms
- X-ray Diffraction in Crystallography
- Infant Nutrition and Health
Children's Hospital of Los Angeles
2016-2024
Sun Moon University
2021-2024
Seoul National University
2007-2022
University of California, Davis
2019-2021
Food and Nutrition Service
2020
University of Georgia
2016-2018
National Cancer Center
2018
The University of Texas MD Anderson Cancer Center
2013-2018
Northwestern University
2013-2016
Korea University
2013-2016
Wnt/beta-catenin signaling plays a central role in development and is also involved diverse array of diseases. Binding Wnts to the coreceptors Frizzled LRP6/5 leads phosphorylation PPPSPxS motifs intracellular region inhibition GSK3beta bound scaffold protein Axin. However, it remains unknown how specifically inhibited upon Wnt stimulation. Here, we show that overexpression LRP6 containing Ser/Thr rich cluster motif impairs activity cells. Synthetic peptides strongly inhibit vitro only when...
Obesity is associated with consumption of energy-dense diets and development systemic inflammation. Gut microbiota play a role in energy harvest inflammation can influence the change from lean to obese phenotypes. The nucleus solitary tract (NTS) brain target for gastrointestinal signals modulating satiety alterations gut-brain vagal pathway may promote overeating obesity. Therefore, we tested hypothesis that high-fat diet‑induced changes gut alter communication increased body fat...
Abstract Cancer stem cells (CSCs) contribute to tumour heterogeneity, therapy resistance and metastasis. However, the regulatory mechanisms of cancer cell stemness remain elusive. Here we identify PCNA-associated factor (PAF) as a key molecule that controls stemness. PAF is highly expressed in breast but not mammary epithelial (MECs). In MECs, ectopic expression induces anchorage-independent growth CSC marker expression. mouse models, conditional ductal hyperplasia. Moreover, endows MECs...
Wnt2 is implicated in various human cancers. However, it remains unknown how upregulated cancer and contributes to tumorigenesis. Here we found that highly expressed colorectal (CRC) cells. In addition co-expression of with Wnt/β-catenin target genes CRC, knockdown or knockout significantly downregulates gene expression CRC Importantly, depletion ablation endogenous inhibits cell proliferation. Similarly, neutralizing secreted reduces Wnt suppresses Conversely, increases proliferation...
Significance Retinoblastoma is a childhood tumor that forms in response to mutations the RB1 gene and loss of functional RB protein. Prior studies suggested retinoblastomas arise from cone photoreceptor precursors, whereas mouse models yield tumors deriving other retinal cell types lacking human retinoblastoma features. We show cultured retinae, initiate RB-depleted precursors are specific maturation state form premalignant retinomas prior lesions, as believed occur patients. In contrast,...
Imbalances in gut microbiota and subsequent destabilization of intestinal barrier equilibrium have been related to the evolution metabolic disorders. Goji berries (Lycium barbarum; GB) their fermented counterpart (FGB) identified for prebiotic capacity managing functions inflammatory profiles Consequently, this research was designed investigate effects supplementing GB FGB on integrity, inflammation, changes composition high-fat (HF)-fed rats.
Deregulation of mitogen-activated protein kinase (MAPK) signaling leads to development pancreatic cancer. Although Ras-mutation-driven tumorigenesis is well understood, the underlying mechanism Ras-independent MAPK hyperactivation remains elusive. Here, we have identified a distinct function PCNA-associated factor (PAF) in modulating signaling. PAF overexpressed cancer and required for cell proliferation. In mouse models, expression induced intraepithelial neoplasia with stem markers....
Development of obesity-associated comorbidities is related to chronic inflammation, which has been linked gut microbiota dysbiosis. Thus, modulating composition could have positive effects for metabolic disorders, supporting the use probiotics as potential therapeutics in vivo, may be enhanced by a microencapsulation technique. Here we investigated non-encapsulated or pectin-encapsulated probiotic supplementation (Lactobacillus paracasei subsp. L. casei W8®; W8) on and profile high-fat (HF)...
Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis. Manipulations of the microbiota its metabolites are attractive targets for obesity treatment. The predominant oligosaccharide found human milk, acts as a prebiotic with beneficial effects on host. However, little known about 2′-FL obesity. aim this study was determine supplementation microbiota-gut-brain axis diet-induced obese phenotype high...
Genetic mutation of α-synuclein (α-SYN) is clearly verified as the causal factor human and mouse Parkinson's disease. However, biological function α-SYN has not been demonstrated until now. In this investigation, we reveal that a co-regulator growth factor-induced AKT activation. Elimination SYN reduces IGF-1-mediated Similarly, mutant suppresses IGF-1-induced Wild-type can interact with enhance solubility plasma localization in response to IGF-1, whereas α-SYNs do AKT. addition, elevated...
We obtained a new metastable packing polymorph of donepezil, designated as form K, epitaxially grown on substrate crystals the more stable F, or solely by increasing concentration. Although donepezil is highly flexible molecule, crystal structure analysis reveals that molecular conformation same in F. Donepezil does not hydrogen bonds and has no appreciable electrostatic interactions. Form K can be homogeneously, i.e., without seeds, from supersaturated solutions (S > 12). However, presence...
2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects host. It previously been shown that 2'-FL can improve metabolic phenotype high-fat (HF)-fed mice. Here we investigated whether dietary supplementation with was associated improved intestinal barrier integrity, signaling vagal afferent pathway cognitive function. Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% diet without (10% w/w) for...
Donepezil is used for the palliative treatment of mild to moderate dementia Alzheimer's disease. crystallized as four solvent-free polymorphs including forms I, II, C, and F which differ in molecular conformations packing. Conformational difference comes from torsion 2, 3-dihydroinden-1-one moiety methyl-benzyl ring with respect piperidyl ring. Similar melting temperatures heats fusion were observed polymorphic made polymorph selection by solvent crystallization method poor. The relative...
Rac is a protein involved in the various functions of macrophages (Mφ), including production reactive oxygen species (ROS), phagocytosis, chemotaxis and secretion cytokines (such as γ-INF). This study tested effects nucleosides containing 8-oxoguanine(8-hydroxyguanine) such 8-oxo-2′-guanosine (8-oxoG) or 8-oxo-2′-deoxyguanosine (8-oxodG), on above-listed Rac-associated Mφ using mouse peritoneal (MpMφ). It reported that 8-oxodG was able to effectively inhibit MpMφ. Compared 8-oxodG, 8-oxoG...
HMLEs (HMLE-SNAIL and Kras-HMLE, Kras-HMLE-SNAIL pairs) serve as excellent model system to interrogate the effect of SNAIL targeted agents that reverse epithelial-to-mesenchymal transition (EMT). We had earlier developed a SNAIL-p53 interaction inhibitor (GN-25) was shown suppress function. In this report, using systems biology pathway network analysis, we show GN-25 could cause reversal EMT leading mesenchymal-to-epithelial (MET) in well-recognized HMLE-SNAIL models. induced MET found be...
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Background: Simplified hematoxylin and eosin (H&E) staining followed by cryo-sectioning enables rapid identification of cancerous tissue within the procedure room during Mohs micrographic surgery. Yet, a faster evaluation method is desirable as protocol requires physically sectioning after freezing, which leads to prolonged time along with frozen artifacts that may occur in sectioning.