A5077588952- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Carcinogens and Genotoxicity Assessment
- Trace Elements in Health
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Phytochemicals and Antioxidant Activities
- NF-κB Signaling Pathways
- Pharmacological Effects of Natural Compounds
- Gastrointestinal motility and disorders
- RNA modifications and cancer
- Free Radicals and Antioxidants
- Genomics and Chromatin Dynamics
- Cancer therapeutics and mechanisms
- DNA and Nucleic Acid Chemistry
- Ion Channels and Receptors
- Drug Transport and Resistance Mechanisms
- Genetic factors in colorectal cancer
- RNA and protein synthesis mechanisms
- Antioxidant Activity and Oxidative Stress
- Ion Transport and Channel Regulation
Gwangju University
2025
Chosun University
2012-2024
University of South Florida
2016-2020
Kyungnam University
2019
Jeju National University
2012
Washtenaw Community College
2012
University of Michigan–Ann Arbor
2012
UiT The Arctic University of Norway
2012
National Cancer Center
2009
Gwangju Institute of Science and Technology
2006
Aberrant expression of apurinic-apyrimidinic endonuclease–1 (APEX1) has been reported in numerous human solid tumors and is positively correlated with cancer progression; however, the role APEX1 tumor progression poorly defined. Here, we show that contributes to aggressive colon behavior functions as an upstream activator Jagged1/Notch signaling pathway. overexpression or knockdown cell lines induced profound changes malignant properties such proliferation, anchorage-independent growth,...
Saccharomyces cerevisiae possesses two functional homologues (Ntg1p and Ntg2p) of the Escherichia coli endonuclease III protein, a DNA base excision repair N-glycosylase with broad substrate specificity directed primarily against oxidatively damaged pyrimidines. The specificites Ntg1p Ntg2p are similar but not identical, differences in their amino acid sequences as well inducibility by damaging agents suggest that proteins may have different biological roles subcellular locations....
Cytosine deamination to uracil occurs frequently in cellular DNA. In vitro, RNA polymerase efficiently inserts adenine opposite uracil, resulting G A base substitutions. vivo, could potentially alter transcriptional fidelity, production of mutant proteins. This study demonstrates that nondividing Escherichia coli cells, a DNA template replaced with stop codon the firefly luciferase gene results conversion inactive active luciferase. The level substitution is dependent on capacity repair...
Abstract Tumors frequently contain mutations in the ras genes, resulting constitutive activation of Ras-activated signaling pathway. The Ras is involved not only tumor progression but also development resistance cells to platinum-based chemotherapeutic agents. To investigate potential mechanisms underlying this resistance, we analyzed effect activated H-Ras on expression nucleotide excision repair genes. Here identified ERCC1, which one key enzymes repair, as being markedly up-regulated by...
Although capsaicin, a pungent component of red pepper, is known to induce apoptosis in several types cancer cells, the mechanisms underlying capsaicin-induced cytotoxicity are unclear. Here, we showed that dihydrocapsaicin (DHC), an analog potential inducer autophagy. DHC was more cytotoxic than capsaicin HCT116, MCF-7 and WI38 cell lines. Capsaicin did not affect on sub-G1apoptotic peak, but induced G0/G1 arrest HCT116 cells. caused artificial autophagosome marker GFP-LC3 redistribute...
Triphlorethol‐A, phlorotannin found in Ecklonia cava , induced heme oxygenase‐1 (HO‐1) expression at mRNA and protein levels, leading to increased HO‐1 activity. Transcription factor NF‐E2 related 2 (Nrf2) regulates antioxidant response element (ARE) of phase detoxifying enzymes. Triphlorethol‐A nuclear translocation, ARE binding, transcriptional activity Nrf2. exhibited activation ERK U0126, inhibitor kinase, suppressed triphlorethol‐A Nrf2, finally decreased level. Taken together, these...
We demonstrate herein that silibinin, a polyphenolic flavonoid compound isolated from milk thistle (Silybum marianum), inhibits LPS-induced activation of macrophages and production nitric oxide (NO) in RAW 264.7 cells. Western blot analysis showed silibinin iNOS gene expression. RT-PCR iNOS, TNF-α, IL1β. also strongly p38 MAPK phosphorylation, whereas the ERK1/2 JNK pathways are not inhibited. The inhibitor abrogated nitrite production, MEK-1 did affect production. A molecular modeling study...
Homologous recombination (HR) is critical for error-free repair of DNA double-strand breaks. Chromatin loading RAD51, a key protein that mediates the recombination, crucial step in execution HR repair. Here, we present evidence SUMOylation RAD51 recruitment to chromatin and We found topoisomerase 1-binding arginine/serine-rich (TOPORS) induces at lysine residues 57 70 response damaging agents. The was facilitated by an ATM-induced phosphorylation TOPORS threonine 515 upon damage. Knockdown...
Abstract The cell signaling factors EGFR, EphA2, and Ephexin1 are associated with lung colorectal cancer play an important role in tumorigenesis. Although the respective functional roles of EGFR EphA2 well known, interactions between these proteins a for complex is not understood. Here, we showed that Ephexin1, each expressed at higher levels patient tissues, binding to was both increased tumor grade metastatic cases types. Treatment Epidermal Growth Factor (EGF) induced RR domain kinase...
Abstract Wnt signaling is essential for cell growth and tumor formation abnormally activated in colorectal cancer (CRC), contributing to progression; however, the specific role regulatory mechanisms involved development remain unclear. Here, we show that Ephexin1, a guanine nucleotide exchange factor, significantly overexpressed CRC correlated with increased Wnt/β-catenin pathway activity. Through comprehensive analysis, including RNA sequencing data from TCGA functional assays, observed...
Two genes of Saccharomyces cerevisiae, NTG1 and NTG2, encode proteins with a significant sequence homology to the endonuclease III Escherichia coli. The Ntg1 Ntg2 were overexpressed in E.coli purified apparent homogeneity. substrate specificity for modified bases oxidatively damaged DNA was investigated using gas chromatography/isotope-dilution mass spectrometry. used calf-thymus exposed γ-radiation N2O-saturated aqueous solution. results reveal excision by six pyrimidine-derived lesions,...
Ursolic acid (UA), a pentacyclic triterpene acid, is reported to have anti‐tumor activities; however, the mechanism underlying its anti‐tumorigenic effects poorly understood. To further determine of UA, we investigated UA on release nitric oxide (NO) and tumor necrosis factor‐α (TNF‐α), level inducible synthase (iNOS) TNF‐α gene expression in mouse resting macrophages. We found that elicited dose‐dependent increase NO production, iNOS mRNA. Transient electrophoretic mobility shift assays...
Cadmium is a well known human and animal carcinogen ubiquitous contaminant in the environment. Although carcinogenic mechanism of cadmium multifactorial process, oxidative DNA damage believed to be prime importance. In particular, suppresses capacity cells repair damage. this study, treatment led significant increase gamma-ray-induced 8-oxoguanine (8-oxoG) formation. Western blotting semiquantitative reverse transcription-PCR revealed that caused decrease expression level OGG1...