Substrate specificities of FPG protein (also known as formamidopyrimidine DNA glycosylase) and 8-hydroxyguanine endonuclease were compared by using defined duplex oligodeoxynucleotides containing single residues 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-2'-deoxyadenosine (8-oxodA), 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (Me-Fapy). Duplexes 8-oxodG positioned opposite dC, dG, or dT cleaved, whereas single-stranded duplexes 8-oxodG.dA 8-oxodA any the four...

Aberrantly enhanced vascular endothelial growth factor (VEGF) gene expression is associated with increased tumor and metastatic spread of solid malignancies, including human renal carcinomas. Persistent activation STAT3 linked to tumor-associated angiogenesis, but underlying mechanisms remain unclear. Therefore, we examined whether modulates the stability activity hypoxia-inducible factor-1alpha (HIF-1alpha), in turn enhances VEGF expression. We found that was activated ischemic rat kidneys...

Active oxygen species (AOS) such as O2- and H2O2 have been shown to be generated from both gas tar phases of cigarette smoke it has suggested that they are involved in carcinogenesis due smoking. Therefore, we investigated the effect smoking on oxidative DNA damages human peripheral blood cells using 8-hydroxydeoxy-guanosine (8-OH-dG) a marker. From ten healthy male volunteers aged 20-22 years, 5 ml was taken before 10 minutes after 2 cigarettes minutes. After lysis cell membranes leukocyte...

Tumor angiogenesis is required for tumor development and stimulated by angiogenic inducers like VEGF (vascular endothelial growth factor). Our previous study demonstrated that STAT3 (signal transducer activator of transcription 3) up-regulates HIF-1α (hypoxia inducible factor-1α) protein stability enhances HIF-1-mediated expression in hypoxic solid cells, thus suggesting the inhibition signaling may have clinical applications. In this study, we examined vitro vivo, whether caffeic acid (CA)...

Oxidative stress in humans is associated with damage to DNA, proteins, and biological membranes. stress, which often arises as a result of an imbalance the human antioxidant status, has been implicated aging number diseases such cancer, atherosclerosis, rheumatoid arthritis. This study was performed test hypothesis that consumption fruit juices may improve status plasma. Ten healthy men 25–26 years old were recruited for study. After overnight fasting, subjects fed 150 mL juice, blood...

Abstract Tumors frequently contain mutations in the ras genes, resulting constitutive activation of Ras-activated signaling pathway. The Ras is involved not only tumor progression but also development resistance cells to platinum-based chemotherapeutic agents. To investigate potential mechanisms underlying this resistance, we analyzed effect activated H-Ras on expression nucleotide excision repair genes. Here identified ERCC1, which one key enzymes repair, as being markedly up-regulated by...

Aloe vera continues to be used for wound healing as a folk medicine. We previously reported that A. gel has angiogenic activity. In this study, we report upon the isolation of an component β-sitosterol from and examination its effect damaged blood vessels Mongolian gerbil. chick embryo chorioallantoic membrane assay, was found have effect. It enhanced new vessel formation in gerbil brains by ischaemia/reperfusion, especially cingulated cortex septal regions, dose-dependent fashion (up 500...

The renal carcinogen, ferric nitrilotriacetate (Fe-NTA), is known to induce oxidative stress and the subsequent formation of a type DNA damage, 8-hydroxygu-anine (8-OH-Gua), in rat kidney (Umemura et al., 1990). Using an improved isolation method (Nakae 1995), which reduces background level 8-OH-Gua, we found five-fold increase 8-OH-Gua after single i.p. injection Fe-NTA. On basis report that repair activity enhanced cells are exposed due ionizing radiation (Bases 1992), measurement will...

Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8‐hydroxyguanine (oh 8 Gua) residues in various mammalian cells. To detect this activity, used a synthetic double‐stranded single oh Gua at defined position as substrate, and analyzed products digestion by polyacrylamide gel electrophoresis. Two cleavage sites near residue were detected with partially purified fractions from cow brain rat liver, also preparations all tissues examined. These results...

The objective was to analyse the potential of CT distinguish pneumonic-type bronchioloalveolar cell carcinoma (BAC) from infectious pneumonia. study consisted 21 patients with pathologically proven BAC and 30 Both groups had patchy or diffuse consolidation more than half area a lobe lobes on CT. findings in these two were compared regard morphological appearance, including angiogram, air bronchogram, mucous contrast enhancement pattern, pseudocavitation, cavity air–fluid level, location,...

Cadmium is a well known human and animal carcinogen ubiquitous contaminant in the environment. Although carcinogenic mechanism of cadmium multifactorial process, oxidative DNA damage believed to be prime importance. In particular, suppresses capacity cells repair damage. this study, treatment led significant increase gamma-ray-induced 8-oxoguanine (8-oxoG) formation. Western blotting semiquantitative reverse transcription-PCR revealed that caused decrease expression level OGG1...

This study investigated the role of oncogenic H-Ras in DNA repair capacity NIH3T3 cells. Expression dominant-positive (V12-H-Ras) enhanced host cell reactivation luciferase activity from UV-irradiated and cisplatin-treated plasmids also increased unscheduled synthesis following cisplatin or UV treatment observed enhancement was inhibited by transient transfection with dominant-negative (N17-H-Ras) Rac1 (N17-Rac1) plasmids. Moreover, stable (V12-Rac1) further capacity. Because reactive oxygen...

Doxorubicin (DOX), which is widely used in cancer treatment, can induce cardiomyopathy. One of the main mechanisms whereby DOX induces cardiotoxicity involves pyroptosis through NLR family pyrin domain containing 3 (NLRP3) inflammasome and gasdermin D (GSDMD). Increased NAPDH oxidase (NOX) oxidative stress trigger pyroptosis. Exogenous 8-hydroxydeoxyguanosine (8-OHdG) decreases reactive oxygen species (ROS) production by inactivating NOX. Here, we examined whether 8-OHdG treatment attenuate...

To elucidate the roles of 8-hydroxydeoxyguanosine (oh8dG), nucleoside 8-hydroxyguanine (oh8Gua), we examined effects oh8dG upon LPS-induced intercellular adhesion molecule-1 (ICAM-1) expression and underlying mechanisms in brain microglial cells. We found that reduces reactive oxygen species (ROS) production, STAT3 activation, ICAM-1 expression. also suppresses pro-inflammatory cytokines, such as TNF-α, IL-6 IFN-γ. Overexpression dominant negative completely diminshed STAT3-mediated...