A5011960125- Pancreatic function and diabetes
- Xenotransplantation and immune response
- Diabetes and associated disorders
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Mesenchymal stem cell research
- Cannabis and Cannabinoid Research
- Organ Transplantation Techniques and Outcomes
- Diabetes Management and Research
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Corneal Surgery and Treatments
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Dermatology and Skin Diseases
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Sperm and Testicular Function
- Extracellular vesicles in disease
Seoul National University
2016-2025
Seoul National University Hospital
2016-2025
National University College
2004-2024
New Generation University College
2003-2024
Sungkyunkwan University
2023
University Hospital Münster
2023
Samsung Medical Center
2020
Seoul Institute
2018
John Wiley & Sons (Germany)
2017
Fukushima Medical University
2016
Neural stem cell (NSC) transplantation has been investigated as a means to reconstitute the damaged brain after stroke. In this study, however, we effect on acute cerebral and peripheral inflammation intracerebral haemorrhage (ICH). NSCs (H1 clone) from fetal human were injected intravenously (NSCs-iv, 5 million cells) or intracerebrally (NSCs-ic, 1 at 2 24 h collagenase-induced ICH in rat model. Only NSCs-iv-2 resulted fewer initial neurologic deteriorations reduced oedema formation,...
Mesenchymal stem cells (MSCs) can inhibit T cell proliferation; however, the underlying mechanisms are not clear. In this study, we investigated of immunoregulatory activity MSCs on cells. Irradiated co-cultured with either na?ve or pre-activated in a mixed lymphocyte reaction (MLR) significantly suppressed proliferation dose-dependent manner, irrespective allogeneic disparity between responders and MSCs. Transwell assays revealed that suppressive effect was primarily mediated by soluble...
Pig islets are an alternative source for islet transplantation to treat type 1 diabetes (T1D), but reproducible curative potential in the pig-to-nonhuman primate (NHP) model has not been demonstrated. Here, we report that pig grafts survived and maintained normoglycemia >6 months four of five consecutive immunosuppressed NHPs. were isolated from designated pathogen-free (DPF) miniature pigs infused intraportally into streptozotocin-induced diabetic rhesus monkeys under pretreatment with...
Severe hepatic inflammation is a common cause of acute or chronic liver disease. Macrophages are one the key mediators which regulate progress inflammation. Increasing evidence shows that TAM (TYRO3, AXL and MERTK) family RTKs (receptor tyrosine kinases), expressed in macrophages, alleviates inflammatory responses through negative feedback loop. However, functional contribution each member to progression remains elusive. In this study, we explore role individual proteins during autophagy...
REPORT (d) transgenic pig facilities; (e) biomaterials and encapsulation; (f) immunosuppression tolerance induction.The guidance document from the Second Global Consultation (Geneva, 2011) was also included in these discussions.
Abstract Background Efforts to profile atopic dermatitis (AD) tissues have intensified, yet comprehensive analysis of systemic immune landscapes in severe AD remains crucial. Methods Employing single‐cell RNA sequencing, we analyzed over 300,000 peripheral blood mononuclear cells from 12 patients (Eczema area and severity index (EASI) > 21) six healthy controls. Results revealed significant cell shifts patients, including increased Th2 abundance, reduced NK clusters with compromised...
Abstract Purpose: Genetic polymorphisms of DNA repair genes seem to determine the capacity, which in turn may affect risk breast cancer. To evaluate role genetic cancer, we conducted a hospital-based case-control study Korean women. Experimental Design: We included 872 incident cancer cases and 671 controls recruited from several teaching hospitals Seoul 1995 2002. Twelve loci selected were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (XRCC2...
Lymphocyte activation gene-3 (LAG-3; CD223), a structural homolog of CD4, binds to MHC class II molecules. Recent research indicated that signaling mediated by LAG-3 inhibits T cell proliferation, and serves as key surface molecule for the function regulatory cells. Previous reports demonstrated majority is retained in intracellular compartments rapidly translocated upon stimulation. However, mechanism which translocates was unclear. In this study, we examined trafficking human under...
Recent studies have given ample evidence that atopy is associated with a T-cell deficiency. This deficiency appears to be primary and not merely consequence of disease manifestations. Several indicated the defect displays certain selectivity, resulting in imbalance between helper suppressor atopics. Suppressor cells, carrying Fc IgG marker (T gamma cells) or being inducible by concanavalin A, been found deficient, recent data suggest there an T8+ (cytotoxic/suppressor) T4+ (inducer/helper)...
At the time first pancreas transplant was performed by Kelly and Lillehei in 1966, insulin therapy for diabetes generally available but administered a form that is known today as “conventional therapy.”1 In this era, many half of all juvenile onset diabetics did not reach age 55 years. Early mortality from accelerated cardiovascular disease, renal failure, hypoglycemia-related events were commonplace. The early low success rate transplantation comparison also suboptimal. As will be...
Abstract In 2009, the International Xenotransplantation Association ( IXA ) published a consensus document that provided guidelines and “recommendations” not regulations) for those contemplating clinical trials of porcine islet transplantation. These included 's opinion on what constituted “rigorous pre‐clinical studies using most relevant animal models” were based “non‐human primate testing.” We now report our discussion following careful review 2009 as they relate to testing. summary, we...
Islet transplantation is an effective therapy for selected patients with type 1 diabetes labile glycemic control and hypoglycemic unawareness, but donor organs are limited. xenotransplantation using porcine islets will potentially solve this problem. Although successful proof of concept studies clinically inapplicable anti-CD154 monoclonal antibody (mAb) in pig-to-non-human primate (NHP) islet has been demonstrated by several groups worldwide, applicable anti-CD40 (2C10R4) mAb-based have not...
Pancreatic β cell therapy for type 1 diabetes is limited by low survival rate owing to physical stress and aggressive host immune response. In this study, we demonstrate a multilayer hydrogel nanofilm caging strategy capable of protecting cells from high shear reducing response interfering cell-cell interaction. Hydrogel fabricated monophenol-modified glycol chitosan hyaluronic acid that cross-link each other form nanothin film on the surface via tyrosinase-mediated reactions. Furthermore,...
Mesenchymal stem cell (MSC)-derived small extracellular vesicles (MSC-sEVs) are known to exert immunosuppressive functions. This study showed that MSC-sEVs specifically convert T helper 17 (Th17) cells into IL-17 low-producer (ex-Th17) by degrading RAR-related orphan receptor γt (RORγt) at the protein level. In experimental autoimmune encephalomyelitis (EAE)-induced mice, treatment with was found not only ameliorate clinical symptoms but also reduce number of Th17 in draining lymph nodes and...
BackgroundPsoriasis is a chronic inflammatory skin disease with Th17-skewed immune phenotype. Although it has been generally accepted that regulatory T cells (Tregs) in lesional psoriatic have functional impairment due to the local microenvironment, molecular properties of skin-homing Tregs not well explored.MethodsWe designed an extensive 39 marker mass cytometry (CyTOF) panel deeply profile landscape from 31 people psoriasis stratified by area severity index score as mild (n = 15)...
Human thymocytes, unlike mouse express major histocompatibility complex (MHC) class II molecules on their surface, especially during the fetal and perinatal stages. Based this observation, we previously identified a novel developmental pathway for generation of CD4+ T cells via interactions between MHC II–expressing thymocytes (thymocyte–thymocyte [T–T] interactions) with transgenic system. However, dissection T–T interaction in humans has not been possible because lack known cellular...
Comparison of Four Pancreatic Islet Implantation SitesAlthough the liver is most common site for pancreatic islet transplantation, it not optimal.We compared kidney, liver, muscle, and omentum as transplantation sites with regard to operative feasibility, efficiency implantation glycemic control.Islets from C57BL/6 mice were transplanted into diabetic syngeneic recipients.The mean time mortality measured assess feasibility.To efficiency, marginal mass required cure diabetes taken achieve...
Abstract Background Islet allotransplantation is a promising way to treat some type 1 diabetic (T1D) patients with frequent hypoglycemic unawareness, and islet xenotransplantation emerging overcome the problem of donor organ shortage. Our recent study showing reproducible long‐term survival porcine islets in non‐human primates ( NHP s) allows us examine whether autologous regulatory T‐cell (Treg) infusion at peri‐transplantation period would induce transplantation tolerance setting. Methods...