A5087887791- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes and associated disorders
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Cannabis and Cannabinoid Research
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Advanced Nanomaterials in Catalysis
- Hydrogels: synthesis, properties, applications
- Mesenchymal stem cell research
- Advanced MRI Techniques and Applications
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Metabolism, Diabetes, and Cancer
- Bone Tissue Engineering Materials
- Nanocluster Synthesis and Applications
- Adenosine and Purinergic Signaling
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanoparticles: synthesis and applications
- Hemoglobin structure and function
- Dendrimers and Hyperbranched Polymers
- Microfluidic and Capillary Electrophoresis Applications
- Click Chemistry and Applications
University of Florida
2016-2025
University of Miami
2010-2024
University of Florida Health
2019-2021
Technische Universität Braunschweig
2018
Bayer (Germany)
2018
National Institutes of Health
2016
University of Pittsburgh
2016
Harvard University
2016
Massachusetts General Hospital
2016
Joslin Diabetes Center
2016
A major hindrance in engineering tissues containing highly metabolically active cells is the insufficient oxygenation of these implants, which results dying or dysfunctional portions graft. The development methods to increase oxygen availability within tissue-engineered particularly during early engraftment period, would serve allay hypoxia-induced cell death. Herein, we designed and developed a hydrolytically activated oxygen-generating biomaterial form polydimethylsiloxane...
We demonstrate the applicability of sequential Diels-Alder and azide-alkyne [3 + 2] cycloaddition reactions (click chemistry) for immobilization carbohydrates proteins onto a solid surface. An alpha,omega-poly(ethylene glycol) (PEG) linker carrying alkyne cyclodiene terminal groups was synthesized immobilized an N-(epsilon-maleimidocaproyl) (EMC)-functionalized glass slide via aqueous reaction. In process, alkyne-terminated PEGylated surface provided conjugation azide-containing biomolecules...
Significance Cell encapsulation with biocompatible and permeable hydrogels may allow transplantation without immunosuppression. As an alternative to standard microencapsulation approaches that create single-sized capsules around cell clusters of different sizes, we have designed optimized a novel approach for conformal coating islets Langerhans, resulting in thin, complete, uniform coatings similar thickness on differently sized islets. Coated exhibited no delay glucose-stimulated insulin...
Clinical islet transplantation has demonstrated success in treating type 1 diabetes. A current limitation is the intrahepatic portal vein transplant site, which prone to mechanical stress and inflammation. Transplantation of pancreatic islets into alternative sites preferable, but challenging, as it may require a three-dimensional vehicle confer protection confine well-defined, retrievable space where neovascularization can occur. We have fabricated biostable, macroporous scaffolds from...
A customized microphysiological system supports the perifusion and in situ multiparametric assessment of 3D islet organoids.
The transplantation of allogeneic islets in recent clinical trials has shown substantial promise as a therapy for type 1 diabetes; however, long-term insulin independence remains inadequate. This been largely attributed to the current intravascular, hepatic transplant site, which exposes mechanical and inflammatory stresses. A highly macroporous scaffold, housed within an alternative can support ideal environment islet by providing three-dimensional distribution islets, while permitting...
At the time first pancreas transplant was performed by Kelly and Lillehei in 1966, insulin therapy for diabetes generally available but administered a form that is known today as “conventional therapy.”1 In this era, many half of all juvenile onset diabetics did not reach age 55 years. Early mortality from accelerated cardiovascular disease, renal failure, hypoglycemia-related events were commonplace. The early low success rate transplantation comparison also suboptimal. As will be...
To explore the effects immune-isolating encapsulation has on insulin secretion of pancreatic islets and to improve our ability quantitatively describe glucose-stimulated release (GSIR) islets, we conducted dynamic perifusion experiments with isolated human islets. Free (unencapsulated) hydrogel encapsulated were perifused, in parallel, using an automated multi-channel system that allows sample collection high temporal resolution. Results indicated free secrete less per unit mass or islet...
Chemoselective conjugation of an azido-functionalized thrombomodulin to pancreatic islets was achieved by Staudinger ligation a surface-bound bifunctional poly(ethylene glycol) linker. The presence the tethered resulted in significant increase production activated protein C with reduction islet-mediated thrombogenicity. This report highlights potential tissue-targeted chemistry reduce donor cell mediated procoagulant and proinflammatory responses.
In this study, we demonstrate the applicability of functionalized alginate to serve as a platform for covalent cross-linking or immobilization complementary phosphine groups via chemoselective Staudinger ligation scheme. Azide were covalently linked through heterobifunctional polyethylene glycol (PEG) linker and carbodiimide. Degree azide functionalization was varied function carbodiimide concentration determined by proton nuclear magnetic resonance (1H NMR) infrared spectroscopy....
Abstract Macroporous, biostable scaffolds with controlled porous architecture were prepared from poly(dimethylsiloxane) (PDMS) using sodium chloride particles and a solvent casting particulate leaching technique. The effect of size range overall porosity on the resulting structure was evaluated. Results found 90% v/v ranges above 100 μm to have most optimal open framework porosity. Resulting hydrophobic PDMS coated fibronectin evaluated as platform for adherent cell culture human mesenchymal...
Encapsulation of viable tissues via layer-by-layer polymer assembly provides a versatile platform for cell surface engineering, with nanoscale control over the capsule properties. Herein, we report development hyperbranched polymer-based, ultrathin architecture expressing bioorthogonal functionality and tailored physiochemical Random carbodiimide-based condensation 3,5-dicarboxyphenyl glycineamide on alginate yielded highly branched polysaccharide multiple, spatially restricted, readily...