A5055960115- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Congenital heart defects research
- Nuclear Structure and Function
- Muscle Physiology and Disorders
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Reconstructive Surgery and Microvascular Techniques
- DNA Repair Mechanisms
- Health Systems, Economic Evaluations, Quality of Life
- Bone health and osteoporosis research
- Birth, Development, and Health
- Reproductive Biology and Fertility
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Bone health and treatments
- Animal Genetics and Reproduction
- Clinical Nutrition and Gastroenterology
- Cell death mechanisms and regulation
- Systemic Lupus Erythematosus Research
- Hedgehog Signaling Pathway Studies
- Renal and related cancers
Chungbuk National University Hospital
2023-2025
King's College Hospital NHS Foundation Trust
2016-2024
King's College London
2012-2023
Medical College of Wisconsin
2004-2023
Chinese University of Hong Kong
2012-2021
Royal Prince Alfred Hospital
1992-2020
The University of Sydney
2018-2020
Melanoma Institute Australia
2018-2020
University of Hong Kong
2009-2019
Milwaukee VA Medical Center
2016-2018
Loss of emerin, a lamin-binding nuclear membrane protein, causes Emery-Dreifuss muscular dystrophy. We analyzed 13 site-directed mutations, and four disease-causing mutations that do not disrupt emerin stability or localization. show binds directly to barrier-to-autointegration factor (BAF), DNA-bridging this binding BAF requires conserved residues in the LEM-motif emerin. Emerin has two distinct functional domains: LEM-domain at N-terminus, which mediates BAF, second domain central region,...
Mutations in emerin cause the X-linked recessive form of Emery-Dreifuss muscular dystrophy (EDMD). Emerin localizes at inner membrane nuclear envelope (NE) during interphase, and diffuses into ER when NE disassembles mitosis. We analyzed recruitment wildtype mutant GFP-tagged proteins assembly living HeLa cells. During telophase, accumulates briefly ‘core’ region telophase chromosomes, later distributes over entire rim. Barrier-to-autointegration factor (BAF), a protein that binds...
Emerin belongs to the “LEM domain” family of nuclear proteins, which contain a characteristic ∼40-residue LEM motif. The domain mediates direct binding barrier autointegration factor (BAF), conserved 10-kDa chromatin protein essential for embryogenesis in Caenorhabditis elegans. In mammalian cells, BAF recruits emerin during assembly. also decondensation and central region are lamin A, respectively. However, two other regions lacked ascribed functions, suggesting that could have additional...
Emerin and MAN1 are LEM domain-containing integral membrane proteins of the vertebrate nuclear envelope. The function is unknown, whereas emerin known to interact with lamins, barrier-to-autointegration factor (BAF), nesprin-1α, a transcription repressor. Mutations in cause X-linked recessive Emery–Dreifuss muscular dystrophy. homologs both conserved Caenorhabditis elegans , but loss Ce-emerin has no detectable phenotype. We therefore used C. test hypothesis that Ce-MAN1 overlaps...
Traditional medical education methodologies have been dramatically impacted by the introduction of new teaching approaches over past few decades. In particular, "flipped classroom" format has drawn a great deal attention. However, evidence regarding effectiveness flipped model remains limited due to lack outcome-based studies. present study, pilot histology curriculum organ systems was implemented among 24 Chinese Medicine (TCM) students in classroom at Jinan University. As control, another...
Abstract The midterm effects of cardiac telocytes ( CT s) transplantation on myocardial infarction MI ) and the cellular mechanisms involved in beneficial s are not understood. In present study, we have revealed that was able to significantly decrease infarct size improved function 14 weeks after . It has established exerted a protective effect myocardium this maintained for at least weeks. mechanism behind partially attributed increased angiogenesis, reconstruction network decreased...
The effect of basic fibroblast growth factor on the proliferative and chemotactic response cultured rat patellar tendon fibroblasts was studied in an vitro wound closure model. In quiescent confluent culture, a uniform cell free zone, or wound, generated mechanically as wound. width zone measured at 0, 6, 12, 24 hours after injury, presence 2, 10, 50 mg/mL factor. Basic factor, concentration 10 ng/mL, significantly accelerated closure, resulting almost complete by injury. 2 enhanced...
Emerin, MAN1, and LAP2 are integral membrane proteins of the vertebrate nuclear envelope. They share a 43-residue N-terminal motif termed LEM domain. We found three putative domain genes inCaenorhabditis elegans, designated emr-1,lem-2, lem-3. analyzedemr-l, which encodes Ce-emerin, andlem-2, Ce-MAN1. Ce-emerin Ce-MAN1 migrate on SDS-PAGE as 17- 52-kDa proteins, respectively. Based their biochemical extraction properties immunolocalization, both localized at used antibodies against Ce-MAN1,...
Mutations in the Caenorhabditis elegans unc-84 gene cause defects nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at envelope localization of requires Ce-lamin. also during mitosis, remains periphery until late anaphase, similar to known inner membrane proteins. is first detected 26-cell stage thereafter present most cells development adults. properly expressed unc-83 andanc-1 lines, which have phenotypes tounc-84, suggesting neither...
Covalent modifications of the histone tails and cross talk between these are hallmark features gene regulation. The SAGA acetyltransferase complex is one most well-characterized complexes involved in covalent modifications. recent finding that removal ubiquitin group from H2B performed by a component SAGA, Ubp8, intriguing as it assigns two posttranslation modification processes to complex. In this work, we characterize association Ubp8 with effect acetylation deubiquitylation have on...
Bacterial lipopolysaccharide (LPS) is a causative agent of sepsis-induced ileus. Although it known that LPS activates macrophages and initiates inflammation, the consequences on macrophage network potential inflammatory response within intestinal muscularis have not been investigated. This study was designed to identify cellular functional changes in rat after intraperitoneal LPS. Histo- immunohistochemistry were used phenotype leukocytes. Functional alterations determined using an organ...
Nitric oxide (NO) is a messenger molecule of vascular endothelial cells, macrophages, and neurons.Here, we demonstrate that the activity NO synthase increases transiently but dramatically in chick embryonic myoblasts are competent for fusion.This requires Ca2+, calmodulin, NADPH.In addition, increase coincides with an cellular cGMP level.Furthermore, generated by treatment sodium nitroprusside induces precocious myoblast fusion, while pmonomethyl-L-~~nine, competitive inhibitor synthase, or...
Lemierre's disease consists of suppurative thrombophlebitis the IJV in presence oropharyngeal infection and can be complicated by septic pulmonary emboli. If a patient has an or deep neck pain suspicious for thrombosis, CT MRI is warranted to establish diagnosis. Blood cultures should obtained responsible organism. In most cases F. necrophorum, anaerobic bacterium, sepsis. Once diagnosis made, long-term, high-dose intravenous antibiotics with beta-lactamase activity initiated. persistent...
Cohesin is a protein complex known for its essential role in chromosome segregation. However, cohesin and associated factors have additional functions transcription, DNA damage repair, condensation. The human cohesinopathy diseases are thought to stem not from defects segregation but gene expression. of expression well understood. We used budding yeast strains bearing mutations analogous the disease alleles under control their native promoter study These do significantly affect...
To evaluate both direct and indirect costs of systemic lupus erythematosus (SLE) patients with without flares from a societal perspective, to investigate the impact severity clinical manifestations on direct/indirect costs.A retrospective cost-of-illness study was performed 306 SLE patients. Participants completed questionnaires sociodemographics, employment status, out-of-pocket expenses. Health resources consumption recorded by chart review patient self-reported questionnaire. The total...