A5042988206- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Plant Molecular Biology Research
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Caching and Content Delivery
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Underwater Acoustics Research
- Hippo pathway signaling and YAP/TAZ
- Cooperative Communication and Network Coding
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- RNA regulation and disease
- Advanced Electron Microscopy Techniques and Applications
- Plant Gene Expression Analysis
Shanghai Jiao Tong University
2016-2025
The University of Texas Southwestern Medical Center
2011-2024
Howard Hughes Medical Institute
2003-2024
Qingdao University
2013-2024
Ruijin Hospital
2024
Affiliated Hospital of Qingdao University
2024
Collaborative Innovation Centre for Advanced Ship and Deep-Sea Exploration
2021
China University of Geosciences
2021
Zhengzhou University
2018
Communications Technology Laboratory
2017
The histone methyltransferase Set2, which specifically methylates lysine 36 of H3, has been shown to repress transcription upon tethering a heterologous promoter. However, the mechanism targeting and consequence Set2-dependent methylation have yet be demonstrated. We sought identify protein components associated with Set2 gain some insights into <i>in vivo</i> function this protein. Mass spectrometry analysis complex, purified using tandem affinity method, revealed that RNA polymerase II...
The yeast histone variant H2AZ (Htz1) is implicated in transcription activation, prevention of the ectopic spread heterochromatin, and genome integrity. Our genome-wide localization analysis revealed that Htz1 widely, but nonrandomly, distributed throughout an SWR1-dependent manner. We found enriched intergenic regions compared with coding regions. Its occupancy inversely proportional to rates enrichment RNA polymerase II under different growth conditions. However, does not seem directly...
The presence of Set2-mediated methylation H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets Rpd3S complex to deacetylate transcribed regions and suppress cryptic initiation at certain genes. Here, using a genome-wide approach, we report that Set2–Rpd3S pathway is generally required for controlling acetylation coding regions. When as functional readout this pathway, discovered longer genes and, surprisingly, lower exhibit stronger dependency....
The master spindle checkpoint kinase Mps1 senses kinetochore-microtubule attachment and promotes signaling to ensure accurate chromosome segregation. kinetochore scaffold Knl1, when phosphorylated by Mps1, recruits complexes Bub1–Bub3 BubR1–Bub3 unattached kinetochores. Active ultimately enhances the assembly of mitotic complex (MCC) consisting BubR1–Bub3, Mad2, Cdc20, which inhibits anaphase-promoting or cyclosome bound Cdc20 (APC/CCdc20) delay anaphase onset. Using in vitro reconstitution,...
The spindle checkpoint senses unattached kinetochores and inhibits the Cdc20-bound anaphase-promoting complex or cyclosome (APC/C), to delay anaphase, thereby preventing aneuploidy. A critical inhibitor of APC/C(Cdc20) is mitotic (MCC). It unclear whether MCC suffices inhibit all cellular APC/C. Here we show that human kinase Bub1 not only directly phosphorylates Cdc20, but also scaffolds Plk1-mediated phosphorylation Cdc20. Phosphorylation Cdc20 by Bub1-Plk1 in vitro required for signalling...
Recently synthesized nanotwinned diamond (NTD) exhibits unprecedented Vickers hardness exceeding 200 GPa [Q. Huang et al., Nature (London) 510, 250 (2014)]. This extraordinary finding challenges the prevailing understanding of material deformation and stress response under extreme loading conditions. Here we unveil by first-principles calculations a novel indenter-deformation generated confinement mechanism that suppresses graphitization or bond collapse failure modes commonly known in...
Abstract Context-dependent dynamic histone modifications constitute a key epigenetic mechanism in gene regulation 1–4 . The Rpd3 small (Rpd3S) complex recognizes H3 trimethylation on lysine 36 (H3K36me3) and deacetylates histones H4 at multiple sites across transcribed regions 5–7 Here we solved the cryo-electron microscopy structures of Saccharomyces cerevisiae Rpd3S its free H3K36me3 nucleosome-bound states. We demonstrated unique architecture Rpd3S, which two copies Eaf3–Rco1 heterodimers...
Despite recent studies implicating liquid-like biomolecular condensates in diverse cellular processes, many exist a solid-like state, and their function regulation are less understood. We show that the tumor suppressor Merlin, an upstream regulator of Hippo pathway, localizes to both cell junctions medial apical cortex
Although variants have been identified for every class of histone, their functions remain unknown.We studying the histone H2A variant hv1 in ciliated protozoan Tetrahymena thermophila.Sequence analysis indicates that belongs to H2A.F/Z type variants.On basis high degree evolutionary conservation this histones, they are proposed one or more distinct and essential cannot be performed by major counterparts.Considerable evidence supports hypothesis protein T. thermophila hv1-like proteins other...
The yeast SAS (Something About Silencing) complex and the histone variant H2A.Z have both previously been linked to an antisilencing function at subtelomeric regions. is H4 Lys 16-specific acetyltransferase complex. Here we demonstrate that 16 acetylation by required for efficient incorporation near telomeres. presence of synergistically prevent ectopic propagation heterochromatin. Overall, our data suggest a novel mechanism