A5040431608- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- DNA Repair Mechanisms
- Adipose Tissue and Metabolism
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Sperm and Testicular Function
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics and Neurodevelopmental Disorders
- Microbial Metabolism and Applications
- Reproductive Health and Technologies
- Neurogenetic and Muscular Disorders Research
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Receptor Mechanisms and Signaling
- Genomic variations and chromosomal abnormalities
- Nanoplatforms for cancer theranostics
- Phytoestrogen effects and research
- Wnt/β-catenin signaling in development and cancer
Chengdu Women's and Children's Central Hospital
2024-2025
Chengdu Medical College
2024-2025
Baylor College of Medicine
2008-2024
Northeast Forestry University
2012
University of Science and Technology of China
2008-2011
Hefei National Center for Physical Sciences at Nanoscale
2008-2011
University of Kentucky
2001
In quantitative mass spectrometry, the method by which peptides are grouped into proteins can have dramatic effects on downstream analyses. Here we describe gpGrouper, an inference and quantitation algorithm that offers alternative for assignment of protein groups gene locus improves pseudo-absolute iBAQ weighted distribution shared peptide areas. We experimentally show distributing quantities based unique peak ratios accuracy compared with conventional winner-take-all scenarios....
DNA single-strand break repair (SSBR) is important for maintaining genome stability and homeostasis. The current SSBR model derived from an in vitro-reconstituted reaction suggests that the complex mediated by X-ray cross-complementing protein 1 (XRCC1) assembled sequentially at site of damage. In this study, we provide biochemical data to demonstrate two preformed XRCC1 complexes exist cycling HeLa cells. One contains known enzymes are SSBR, including ligase 3 (DNL3), polynucleotide kinase...
DNA damage response is crucial for maintaining genomic integrity and preventing cancer by coordinating the activation of checkpoints repair damaged DNA. Central to are two checkpoint kinases ATM ATR that phosphorylate a wide range substrates. RING finger WD repeat domain 3 (RFWD3) was initially identified as substrate ATM/ATR from proteomic screen. Subsequent studies showed RFWD3 an E3 ubiquitin ligase ubiquitinates p53 in vitro positively regulates levels damage. We report here associates...
Significance Mass spectrometry-based proteogenomics of patient-derived xenografts (PDXs) identified dihydropyrimidinase-like-3 (DPYSL3) as a multilevel (RNA/protein/phosphoprotein) expression outlier specific to claudin-low (CLOW) PDX. DPYSL3 has established functions in neural cell migration and axon outgrowth but is understudied breast cancer. Here, we demonstrate that loss promotes cell-cycle arrest, multinucleation, collapse the vimentin microfilament network associated with increased...
This retrospective study aimed to examine the influence of hormonal and metabolic parameters across varying body mass index (BMI) levels on embryo quality pregnancy outcomes in fresh transfer cycles using assisted reproductive technology (ART) patients diagnosed with polycystic ovary syndrome (PCOS). A total 167 women PCOS 266 without (control group) were included. Metabolic was compared between two groups evaluate their relationship outcomes. Subgroup analyses performed assess these effects...
Spindle assembly checkpoint (SAC) ensures bipolar attachment of chromosomes to the mitotic spindle and is essential for faithful chromosome segregation, thereby preventing instability (CIN). Genetic evidence suggests a causal link between compromised SAC, CIN, cancer. Bloom syndrome (BS) genetic disorder that predisposes affected individuals BS cells exhibit elevated rates sister chromatid exchange, breaks, CIN. The gene product, BLM, member RecQ helicases are required maintenance genome...
Short repetitive sequences are common in the human genome, and many fall within transcription units. We have previously shown that through CAG repeat tracts destabilizes them a way depends on transcription-coupled nucleotide excision repair mismatch repair. Recent observations antisense accompanies sense genes led us to test effects of triplet instability cells. Here, we report simultaneous (convergent transcription) initiated from two inducible promoters flanking CAG95 tract nonessential...
We previously showed that
Abstract Background The importance of phosphorylation in sperm during spermatogenesis has not been pursued extensively. Testis‐specific serine kinase 3 ( Tssk3 ) is a conserved gene, but TSSK3 functions and substrates are known. Objective goals our studies were to understand the mechanism action TSSK3. Materials methods We analyzed localization sperm, used CRISPR/Cas9 generate knockout (KO) mice which nearly all open reading frame was deleted (ensuring it null mutation), fertility KO by...
Lipid metabolism plays a central role in prostate cancer. To date, the major focus has centered on de novo lipogenesis and lipid uptake cancer, but inhibitors of these processes have not benefited patients. A better understanding how cancer cells access lipids once they are created or taken up stored could uncover more effective strategies to perturb treat Here, we identified that expression adipose triglyceride lipase (ATGL), an enzyme controls droplet homeostasis previously suspected tumor...
During mitosis, chromosomes undergo dynamic structural changes that include condensation of chromosomes-the formation individual compact necessary for faithful segregation sister chromatids in anaphase. Polo-like kinase 1 (Plk1) regulates multiple mitotic events by binding to targeting factors at different structures a phosphorylation dependent manner. In this study, we report the identification putative ATPase targets Plk1 chromosome arms during mitosis. PICH (Plk1-interacting checkpoint...
The muntjacs (Muntiacus, Cervidae) have been extensively studied in terms of chromosomal and karyotypic evolution. However, little is known about their meiotic chromosomes particularly the recombination patterns homologous chromosomes. We used immunostained surface spreads to visualise synaptonemal complexes (SCs), foci kinetochores with antibodies against marker proteins. As other mammals pachytene was longest stage prophase. 39.4% XY bivalents lacked MLH1 compared less than 0.5% autosomes....
Ligand binding to the cell surface receptors initiates signaling cascades that are commonly transduced through a protein-protein interaction (PPI) network activate plethora of response pathways. However, tools capture membrane PPI lacking. Here, we describe cross-linking-aided mass spectrometry workflow for isolation and identification signal-dependent epidermal growth factor receptor (EGFR) proteome. We performed protein cross-linking in culture at various time points following EGF...
Despite 96 million years of evolution separating humans and rodents, 11 closely related reproductive tract-specific genes in humans— SPINT3 , WFDC6 EPPIN WFDC8 WFDC9 WFDC10A WFDC11 WFDC10B WFDC13 SPINT4 WFDC3 —and the 13 orthologous mice, form highly conserved syntenic gene clusters indicative conserved, combined critical functions. Further, despite significant progress toward a nonhormonal male contraceptive targeting protein encoded by one these genes, epididymal peptidase inhibitor...
Abstract The quest for a non-hormonal male contraceptive pill men still exists. Serine protease 37 (PRSS37) is sperm-specific protein that when ablated in mice renders them sterile. In this study we sought to examine the molecular sequelae of PRSS37 loss better understand its function, and determine whether human could rescue sterility phenotype knockout (KO) mice, allowing more appropriate model drug molecule testing. To end, used CRISPR-EZ create lacking entire coding region Prss37 ,...
<p>28 supplementary figures supporting the main text and figures.</p>
<p>IP/MS summary table describing the ATGL interactome in C4-2 cells.</p>
<p>28 supplementary figures supporting the main text and figures.</p>
<p>IP/MS summary table describing the ATGL interactome in C4-2 cells.</p>