A5025247875- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Advanced Electron Microscopy Techniques and Applications
- RNA modifications and cancer
- Flood Risk Assessment and Management
- Hydrology and Sediment Transport Processes
- Fish Biology and Ecology Studies
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Hydrology and Watershed Management Studies
- Fish Ecology and Management Studies
- Marine and fisheries research
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
Southwestern Medical Center
2024
The University of Texas Southwestern Medical Center
2024
Howard Hughes Medical Institute
2024
Dongguan University of Technology
2021-2022
Despite recent studies implicating liquid-like biomolecular condensates in diverse cellular processes, many exist a solid-like state, and their function regulation are less understood. We show that the tumor suppressor Merlin, an upstream regulator of Hippo pathway, localizes to both cell junctions medial apical cortex
Biomolecular condensates, compartments that concentrate molecules without surrounding membranes, are integral to numerous cellular processes. The structure and interaction networks of within condensates remain poorly understood. Using cryo-electron tomography molecular dynamics simulations we elucidated the phase separated chromatin across scales, from individual amino acids network architecture. We found internucleosomal DNA linker length controls nucleosome arrangement histone tail...
Cryo-Focused Ion Beam-Electron Tomography (cryo-FIB-ET) is quickly becoming a powerful tool for investigating structure- function relationships in native environments, enabling the discovery of new biology. However, this technique often limited to visual identification unambiguously large macromolecules (whole organelles, ribosomes, nuclear pore complex, proteasomes), or through matching high resolution signatures (2D template matching) sufficient molecular weight. The need additional...
Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation function remain incompletely understood due difficulties in visualizing condensate interior at high resolution. Here we analyzed structure of biochemically reconstituted chromatin through cryo-electron tomography. We found that traditional blotting methods sample preparation were inadequate, high-pressure freezing plus focused ion beam milling was...
Hydrologic (water temperature) and hydrodynamic depth, flow velocity, Froude number) factors affect fish spawning activities, grounds provide suitable hydrologic conditions for to occur. However, locating is encumbered by uncertainty, particularly pelagic spawners. This may be because such species have unique requirements during their periods, resulting in the wide-ranging approaches used locate grounds. Accordingly, this study was designed accurately through means of spatial suitability...
In situ cryo-Electron Microscopy (cryo-EM) enables the direct interrogation of structure-function relationships by resolving macromolecular structures in their native cellular environment. Tremendous progress sample preparation, imaging and data processing over past decade has contributed to identification determination large biomolecular complexes. However, majority proteins are a size that still eludes cryo-EM data, most exist low copy numbers. Therefore, novel tools needed for identify...
Analysis of the spatial variations in river networks and related influencing factors is crucial for management protection basins. To gain insight into between large basins, this study, three basins from north to south China (Songhua River Basin, Yellow Basin Pearl Basin) were selected investigation. First, based on a digital elevation model, different with six drainage accumulation thresholds extracted using ArcGIS. The optimal determined through fitting relationship threshold density. Then,...