A5059776678- Advanced Electron Microscopy Techniques and Applications
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Electron and X-Ray Spectroscopy Techniques
- Analytical Chemistry and Sensors
- Aluminum Alloy Microstructure Properties
- Advanced X-ray Imaging Techniques
- Advanced biosensing and bioanalysis techniques
- Conducting polymers and applications
- Graphene research and applications
- Solidification and crystal growth phenomena
- Chemical synthesis and alkaloids
- Aluminum Alloys Composites Properties
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- nanoparticles nucleation surface interactions
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- 2D Materials and Applications
- Neuroscience and Neural Engineering
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Graphite, nuclear technology, radiation studies
- Protist diversity and phylogeny
- Advanced ceramic materials synthesis
Howard Hughes Medical Institute
2020-2024
Janelia Research Campus
2020-2024
Helix (United States)
2020-2024
East China University of Science and Technology
2019-2023
Beijing Graphene Institute
2020-2023
Tsinghua University
2017-2022
State Key Laboratory of New Ceramics and Fine Processing
2017-2022
Shanghai Institute of Organic Chemistry
2015-2022
Chinese Academy of Sciences
2011-2022
Jinggangshan University
2020-2021
Abstract The availability of graphene and other two-dimensional (2D) materials on a wide range substrates forms the basis for large-area applications, such as integration with silicon-based technologies, which requires silicon outperforming carrier mobilities. However, 2D were only produced limited archetypal by chemical vapor deposition approaches. Reliable after-growth transfer techniques, that do not produce cracks, contamination, wrinkles, are critical layering onto arbitrary substrates....
The severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) and SARS-CoV-1 accessory protein Orf3a colocalizes with markers of the plasma membrane, endocytic pathway, Golgi apparatus. Some reports have led to annotation both proteins as viroporins. Here, we show that neither SARS-CoV-2 nor form functional ion conducting pores conductances measured are common contaminants in overexpression high levels reconstitution studies. Cryo-EM structures display a narrow constriction...
The flagella of mammalian sperm display non-planar, asymmetric beating, in contrast to the planar, symmetric beating from sea urchin and unicellular organisms. molecular basis this difference is unclear. Here, we perform situ cryo-electron tomography mouse human sperm, providing highest-resolution structural information date. Our subtomogram averages reveal sperm-specific protein complexes within microtubules, radial spokes nexin-dynein regulatory complexes. locations structures these...
Abstract Liquid layering at heterogeneous solid/liquid interfaces is a general phenomenon, which provides structural templates for nucleation of crystalline phases on potent nucleants. However, its efficacy near poor nucleants incompletely understood. Here we use combination X-ray crystal truncation rod analysis and ab initio molecular dynamics to probe the pre-nucleation liquid sapphire–aluminium interface. At sapphire side, ~1.6 aluminium-terminated structure develops, two layers emerge...
Abstract Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, homogeneity thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one main challenges for high-resolution cryo-EM. Here we found uniformity relies on surface flatness supporting film, developed method use ultraflat graphene (UFG) as support cryo-EM achieve...
Short peptides that can respond to external stimuli have been considered as the preferred building blocks construct hydrogels for biomedical applications. In particular, photoresponsive are capable of triggering formation upon light irradiation allow properties be changed remotely by precise and localized actuation. Here, we used photochemical reaction 2-nitrobenzyl ester group (NB) develop a facile versatile strategy constructing photoactivated peptide hydrogels. The with high aggregation...
Significance RDR2 is critical for siRNA-directed DNA methylation in Arabidopsis , functioning physical association with DNA-dependent Pol IV to synthesize the second strands of double-stranded siRNA precursors. Base-pairing between template strand transcribed by and nontemplate needed induce arrest IV/RDR2 transcriptional coupling, but how this occurs unknown. We report structure experimental evidence engages its RNA templates initiates transcription. ends RNAs displaced from RNA/DNA...
Viruses use a plethora of mechanisms to evade immune responses. A recent example is neutralization the nuclear DNA cytosine deaminase APOBEC3B by Epstein-Barr virus (EBV) ribonucleotide reductase subunit BORF2. Cryo-EM studies APOBEC3B-BORF2 complexes reveal large >1000-Å 2 binding surface composed multiple structural elements from each protein, which effectively blocks active site accessing single-stranded substrates. Evolutionary optimization suggested unique insertions in BORF2 absent...
RNA-guided systems, such as CRISPR-Cas, combine programmable substrate recognition with enzymatic function, a combination that has been used advantageously to develop powerful molecular technologies
Three tungsten compounds supported Ir catalysts with different reduction degrees, Ir-WO3-x, Ir-W/WO3-x, and Ir-W, are reported for pH-universal water splitting catalysts. The Ir-W/WO3-x optimized local acidic microenvironment Ir...
Diffusion models have demonstrated impressive performance in generating high-quality videos from text prompts or images. However, precise control over the video generation process, such as camera manipulation content editing, remains a significant challenge. Existing methods for controlled are typically limited to single type, lacking flexibility handle diverse demands. In this paper, we introduce Shader (DaS), novel approach that supports multiple tasks within unified architecture. Our key...
Abstract An ionic liquid (i.e., 1‐butyl‐3‐methylimidazolium hexafluorophosphate, BMIMPF 6 )‐single‐walled carbon nanotube (SWNT) gel modified glassy electrode (BMIMPF ‐SWNT/GCE) is fabricated. At it the voltammetric behavior and determination of p ‐nitroaniline (PNA) explored. PNA can exhibit a sensitive cathodic peak at −0.70 V (vs. SCE) in pH 7.0 phosphate buffer solution on electrode, resulting from irreversible reduction PNA. Under optimized conditions, current linear to concentration...
Abstract The first total synthesis of (−)‐hosieine A was accomplished and features an unprecedented nitroso–ene cyclization to construct the 2‐azabicyclo[3.2.1]octane ring system. Phosphine‐enabled stereoselective bromohydrination provided interesting mechanistic insights into anti‐Markovnikov process. Also noteworthy is retention stereochemistry at C9 in facile radical debromination initiated by Et 3 B/air.
Graphene grown on Cu by chemical vapor deposition is rough due to the surface roughening of for releasing interfacial thermal stress and/or graphene bending energy. The roughness degrades electrical conductance and mechanical strength graphene. Here, using vicinal Cu(111) flat as model substrates, we investigated critical role original topography deformation covered We demonstrated that terrace steps dominate formation step bunches (SBs). Atomically with down 0.2 nm was films. When...