A5005741943- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Pancreatic and Hepatic Oncology Research
- Cancer-related Molecular Pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- interferon and immune responses
- Kruppel-like factors research
- MicroRNA in disease regulation
- Prenatal Screening and Diagnostics
- Cancer Mechanisms and Therapy
Hainan Medical University
2012-2025
Hainan Medical College Hospital
2015-2024
Southern University of Science and Technology
2024
ORCID
2021
Jinan University
2018
Guangzhou Research and Creativity Biotechnology (China)
2018
Shanghai Ninth People's Hospital
2017
Shanghai Jiao Tong University
2017
Hainan University
2015
Guangxi Medical University
2015
Abstract A high level of serum alpha fetoprotein ( AFP ) is positively associated with human hepatocellular carcinoma HCC carcinogenesis and metastasis; however, the function in metastasis unknown. This study has explored effects on regulating metastatic invasive capacity cells. Forty‐seven clinical patients' liver samples were collected diagnosed; cells line, Bel 7402 ‐producing) cancer cell line (non‐ selected to analyse role The results indicated that concentration was correlated...
Recent evidences indicated that some local anaesthetic agents played a role in inhibiting the proliferation of cancer cells; Whether ropivacaine is able to promote apoptosis hepatocellular carcinoma (HCC) cells still unclear. The aim this study was investigate effect on HCC cells.In present study, we treated cell lines, Bel7402 and HLE with ropivacaine. MTT, DAPI stain, trypan blue exclusion dye assay, flow cytometry, electron microscopy, computational simulation, laser confocal microscope,...
Abstract Alpha-fetoprotein (AFP) has been recognized as a key regulator of cell proliferation in hepatocellular carcinoma (HCC). However, whether AFP functions cancer autophagy remains unknown. This study investigated the effects on HCC cells. The role was studied two lines, PLC/PRF/5 and HLE. Cell autophagy, apoptosis, proliferation, migration invasion were analysed with Western blotting, co-immunoprecipitation (CoIP), immunofluorescence, animal models, MTT assays, flow cytometry (FCM),...
Abstract Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, homogeneity thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one main challenges for high-resolution cryo-EM. Here we found uniformity relies on surface flatness supporting film, developed method use ultraflat graphene (UFG) as support cryo-EM achieve...
Alpha-fetoprotein(AFP) is a cancer biomarker for the diagnosis of hepatocellular carcinoma(HCC); however, its role in macrophage polarization and phagocytosis remains unclear. In present study, we explored correlation between AFP regulation function possible regulatory mechanisms. Human mononuclear leukemia cells (THP-1) monocytes from healthy donors were used to analyze effect on macrophages’ phenotype phagocytosis. THP-1 human donor-derived polarized into M0 macrophages induced by phorbol...
Despite its well-defined role as a serum growth factor during fetal liver development and hepatic oncogenesis, the biological significance of cytoplasmic alpha-fetoprotein (AFP) remains incompletely understood. Here, we provide evidence to illustrate that AFP may function regulator in phosphatidylinositol 3-kinase (PI3K)/AKT pathway human hepatocellular carcinoma cells. The results demonstrated colocalization interaction phosphatase tensin homolog deleted on chromosome 10 (PTEN) cytoplasm...
Hepatitis B virus (HBV)-X protein (HBx) plays critical role in inducing the malignant transformation of liver cells. Alpha fetoprotein (AFP) expression is closely related to hepatocarcinogenesis. We report that Oct4, Klf4, Sox2 and c-myc positively associated with AFP(+)/HBV(+) hepatocellular carcinoma(HCC) tissues, stemness markers CD44, CD133 EpCAM was significantly higher HCC tissues compared normal or AFP (-)/HBV(-) tissues. turned on prior c-myc, human L-02 cell line CHL lines upon...
// Mingyue Zhu 1, 2, * , Junli Guo 3, Wei Li 2 Yan Lu Shigan Fu 1 Xieju Xie 4 Hua Xia Xu Dong Yi Chen Ming Quan 3 Shaojiang Zheng 5 Keping Mengsen Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Medical College, Haikou, 571199, P. R. China Molecular Biology, Department Gastroenterology, Hepatology & Nutrition, The University Texas MD Anderson Cancer Center, Houston, TX 77030, USA Physiology Pathophysiology, Tumor Institute, Affiliated Hospital 570102, These authors...
AIM:The goal of this study was to characterize the AFP receptor, its possible signal transduction pathway and proliferative functions in human hepatoma cell line Bel 7402. METHODS: Cell proliferation enhanced by detected MTT assay, 3 H-thymidine incorporation S-stage percentage cycle analysis.With radioactive labeled 125 I-AFP for receptor binding assay; cAMP accumulation, protein kinase A activity were immunosorbent assay change intracellular free calcium ([Ca 2+ ]i) monitored scanning...
Although there is increasing evidence that alpha fetoprotein (AFP) may function as regulatory factor in the growth of tumor cells, precise mechanism still unclear. In current study, we investigated role cytoplasmic AFP caspase-3-mediated signaling apoptosis. Our results showed low doses TNF-related apoptosis-inducing ligand (TRAIL) elevated activity caspase-8, but not caspase-3. Caspase-3 colocalized and interacted with cytoplasm Bel 7402 translocated into nuclei association occurrence...
Hepatocellular carcinoma (HCC) is a prevalent malignant cancer worldwide, characterized by high morbidity and mortality rates. Alpha-fetoprotein (AFP) glycoprotein synthesized the liver yolk sac during fetal development. However, serum levels of AFP exhibit significant correlation with onset progression HCC in adults. Extensive research has demonstrated that tumor microenvironment (TME) plays crucial role transformation HCC, key factor TME, promoting The objective this review was to analyze...
Abstract Alpha-fetoprotein (AFP), a serum glycoprotein, is expressed during embryonic development and the pathogenesis of liver cancer. It serves as clinical tumor marker, function carcinogen, immune suppressor, transport vehicle; but detailed AFP structural information has not yet been reported. In this study, we used single-particle cryo-electron microscopy(cryo-EM) to analyze structure recombinant obtained 3.31 Å cryo-EM built an atomic model AFP. We observed identified certain features...
The transplantation of cardiac stem cells (CSCs) is thought to be responsible for improving the performance injured heart induced by myocardial infarction (MI). However, mechanisms involved in migration activated CSCs post‑MI remain clarified. In this study, were isolated from rat hearts and a cellular assay was performed using 24‑well Transwell system. Stem cell factor (SCF) CSC concentration‑dependent manner, which could blocked with an SCF antibody as well PI3K/AKT inhibitor, LY294002....
CXCR4, stromal cell-derived factor-1α(SDF 1α) receptor, stimulates growth and metastasis of hepatocellular carcinoma (HCC). Alpha-fetoprotein(AFP) governs the expression some metastasis-related genes. Here we report that AFP CXCR4 levels correlated in HCC tissues. AFP-expressing vectors induced CXCR4. In agreement, depletion by siRNA decreased co-localized interacted with PTEN, thus inducing activating AKT(Ser473) phosphorylation. turn, phospho-mTOR(Ser2448) entered nucleus bound gene...
// Yi Chen 1, 2, * , Haixia Xu Mingyue Zhu 2 Kun Liu Bo Lin Ruxian Luo 3 Chuanbai and Mengsen Li 1 Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, Province, P. R. China Provincial Carcinogenesis Intervention, Department Psychiatry, Anning Hospital, These authors have contributed equally to this work are co-first Correspondence to: Li, email: mengsenli@163.com Chen, 812450325@qq.com Keywords: depressive model, tryptophan hydroxylase, epigenetics Received: March 14,...
Hepatitis B virus (HBV)-X protein(HBx) is a transactivator of host several cellular genes including alpha-fetoprotein(AFP) and AFP receptor(AFPR) which contributes to HBV-associated tumor development. The expression AFP/AFPR are correlated with hepatocellular carcinoma(HCC)-initial cells. But the role AFPR in promoting occurrence HBV-related HCC were still unclear. A total 71 clinical patients' liver specimens, normal human cells L-02 cell lines, PLC/PRF/5 selected for analyzing effects HBx...
Despite consideration of benzyl isothiocyanate(BITC) is applied to prevention and therapeutic cancer, the role BITC in inducing apoptosis, inhibiting migration invasion hepatocellular carcinoma(HCC) cells still unclear. In this study, we aim explore effects on growth, HCC vitro. When human cell lines, Bel 7402 HLE, were treated with an optimal concentration for 48 hours, results indicated that inhibits growth promotes apoptosis cells; has a significant inhibitory effect cells. stimulated...
Alpha‐fetoprotein (AFP) is an early serum growth factor in the foetal liver development and hepatic carcinogenesis; However, precise biological role of cytoplasmic AFP remains elusive. Although we recently demonstrated that might interact with caspase‐3 inhibit signal transduction apoptosis human hepatocellular carcinoma (HCC) cells, details this interaction are not clear. To reveal molecular relationship between caspase‐3, performed docking, co‐immunoprecipitation (Co‐IP), laser confocal...
Ginger, a popular functional food, has been widely used throughout the world for centuries. However, its metabolic behaviors remain unclear, which entails an obstacle to further understanding of components. In this study, profiles ginger in rats were systemically investigated by UPLC–Q/TOF-MS. The results included characterization 92 components based on summarized fragmentation patterns and self-building chemical database. Furthermore, four representative compounds selected explore typical...