A5100657439- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Nicotinic Acetylcholine Receptors Study
- Epigenetics and DNA Methylation
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Hepatocellular Carcinoma Treatment and Prognosis
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- Cell death mechanisms and regulation
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Oral microbiology and periodontitis research
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Advanced Biosensing Techniques and Applications
- interferon and immune responses
- Hepatitis B Virus Studies
- Immune cells in cancer
- Carbon dioxide utilization in catalysis
Sun Yat-sen University
2021-2025
Peking University Shenzhen Hospital
2020-2025
University of Hong Kong - Shenzhen Hospital
2025
Zhongshan Hospital
2025
Fudan University
2025
OmniActive Health Technologies (United States)
2023-2025
The First Affiliated Hospital, Sun Yat-sen University
2024-2025
Ningbo University
2025
Zhejiang University
2024-2025
Hainan Medical College Hospital
2015-2024
Abstract The heterogeneity of macrophages influences the response to immune checkpoint inhibitor (ICI) therapy. However, few studies explore impact APOE + on ICI therapy using single‐cell RNA sequencing (scRNA‐seq) and machine learning methods. scRNA‐seq bulk RNA‐seq data are Integrated construct an M.Sig model for predicting based distinct molecular signatures macrophage algorithms. Comprehensive analysis as well in vivo vitro experiments applied potential mechanisms affecting response....
Abstract A high level of serum alpha fetoprotein ( AFP ) is positively associated with human hepatocellular carcinoma HCC carcinogenesis and metastasis; however, the function in metastasis unknown. This study has explored effects on regulating metastatic invasive capacity cells. Forty‐seven clinical patients' liver samples were collected diagnosed; cells line, Bel 7402 ‐producing) cancer cell line (non‐ selected to analyse role The results indicated that concentration was correlated...
Recent evidences indicated that some local anaesthetic agents played a role in inhibiting the proliferation of cancer cells; Whether ropivacaine is able to promote apoptosis hepatocellular carcinoma (HCC) cells still unclear. The aim this study was investigate effect on HCC cells.In present study, we treated cell lines, Bel7402 and HLE with ropivacaine. MTT, DAPI stain, trypan blue exclusion dye assay, flow cytometry, electron microscopy, computational simulation, laser confocal microscope,...
Emerging studies have revealed the potent functions of circRNAs in breast cancer tumorigenesis. However, biogenesis, biofunction and mechanism triple-negative (TNBC) are largely unknown.High-throughput RNA sequencing was applied to identify dysregulated TNBCs paired normal tissues. pulldown luciferase assays were performed investigate interaction between circular CD44 (circCD44, also annotated as hsa_circ_0021735) miR-502-5p. RIP used circCD44 IGF2BP2. Cell viability, colony formation,...
Background & Aims The prognoses and therapeutic responses of patients with intrahepatic cholangiocarcinoma (iCCA) depend on spatial interactions among tumor microenvironment (TME) components. However, the TME characteristics iCCA remain poorly understood. aim this study was to generate a comprehensive atlas using artificial intelligence-assisted multiomics patterns identify features associated prognosis immunotherapy. Approach Results: Spatial multiomics, including imaging mass cytometry...
Hepatitis B virus (HBV)-X protein (HBx) plays critical role in inducing the malignant transformation of liver cells. Alpha fetoprotein (AFP) expression is closely related to hepatocarcinogenesis. We report that Oct4, Klf4, Sox2 and c-myc positively associated with AFP(+)/HBV(+) hepatocellular carcinoma(HCC) tissues, stemness markers CD44, CD133 EpCAM was significantly higher HCC tissues compared normal or AFP (-)/HBV(-) tissues. turned on prior c-myc, human L-02 cell line CHL lines upon...
Hepatocellular carcinoma (HCC) is a prevalent malignant cancer worldwide, characterized by high morbidity and mortality rates. Alpha-fetoprotein (AFP) glycoprotein synthesized the liver yolk sac during fetal development. However, serum levels of AFP exhibit significant correlation with onset progression HCC in adults. Extensive research has demonstrated that tumor microenvironment (TME) plays crucial role transformation HCC, key factor TME, promoting The objective this review was to analyze...
Abstract Alpha-fetoprotein (AFP), a serum glycoprotein, is expressed during embryonic development and the pathogenesis of liver cancer. It serves as clinical tumor marker, function carcinogen, immune suppressor, transport vehicle; but detailed AFP structural information has not yet been reported. In this study, we used single-particle cryo-electron microscopy(cryo-EM) to analyze structure recombinant obtained 3.31 Å cryo-EM built an atomic model AFP. We observed identified certain features...
Abstract Acetylcholine binding proteins (AChBPs) are unique spatial homologs of the ligand-binding domains nicotinic acetylcholine receptors (nAChRs), and they reproduce some pharmacological properties nAChRs. X-ray crystal structures AСhBP in complex with α-conotoxins provide important insights into interactions distinct nAChR subtypes. Although considerable efforts have been made to understand why α-conotoxin GIC is strongly selective for α3β2 nAChR, this question has not yet solved. Here...
Systemic sclerosis (SSc) is a prototypic fibrotic disease characterized by localized or diffuse skin thickening and fibrosis. Tissue fibrosis driven myofibroblasts, factors affecting myofibroblast activation may also be involved in the development of SSc. In this study, we examined molecular mechanisms underlying SSc focusing on processes. Bioinformatics analysis conducted to identify differentially expressed miRNAs (DEMs) genes (DEGs) revealed that microRNA-16-5p (miR-16-5p) was...
Despite consideration of benzyl isothiocyanate(BITC) is applied to prevention and therapeutic cancer, the role BITC in inducing apoptosis, inhibiting migration invasion hepatocellular carcinoma(HCC) cells still unclear. In this study, we aim explore effects on growth, HCC vitro. When human cell lines, Bel 7402 HLE, were treated with an optimal concentration for 48 hours, results indicated that inhibits growth promotes apoptosis cells; has a significant inhibitory effect cells. stimulated...
Alpha‐fetoprotein (AFP) is an early serum growth factor in the foetal liver development and hepatic carcinogenesis; However, precise biological role of cytoplasmic AFP remains elusive. Although we recently demonstrated that might interact with caspase‐3 inhibit signal transduction apoptosis human hepatocellular carcinoma (HCC) cells, details this interaction are not clear. To reveal molecular relationship between caspase‐3, performed docking, co‐immunoprecipitation (Co‐IP), laser confocal...
Epithelial membrane protein 1 (EMP1) is a key gene that regulates cell proliferation and metastatic capability in various types of cancer, serves an important role tumor-immune interactions. However, the association between EMP1 clinical prognosis, as well presence tumor-infiltrating lymphocytes bladder urothelial carcinoma (BLCA) remains unclear. The present study aimed to explore relationship expression tumor immune infiltration BLCA. In study, BLCA was analyzed using Oncomine database,...
Abstract Hepatocellular carcinoma (HCC) cell resistance to the effects of paclitaxel has not been adequately addressed. In this study, we found that significantly inhibited viability HLE, Bel 7402 and L-02 cells in a dose- time-dependent manner. HLE resisted cytotoxicity when transfected with pcDNA3.1- afp vectors. However, sensitivity was increased alpha fetoprotein (AFP)-siRNA. associated expression “stemness” markers CD44 CD133. Paclitaxel growth promoted apoptosis by inducing...
Oral squamous cell carcinoma (OSCC) presents a significant health challenge owing to its complex origin and limited treatment success. The precise function of Fanconi anemia complementation group E (FANCE), key gene involved in DNA repair OSCC, remains unclear. present study performed bioinformatics analysis Cancer Genome Atlas dataset cellular experiments demonstrate that FANCE is significantly upregulated OSCC. Enhanced expression was associated with poor survival outcome patients...
ABSTRACT Background Lymph node metastasis critically impacts prognosis in head and neck malignancies. Preoperative imaging (CT/MRI) is vital for assessment but often yields false results. This study examines the concordance between preoperative postoperative pathology identifies factors influencing accuracy. Methods A retrospective cohort from 2014 to 2023 included patients with Clinical radiological data were analyzed, random forest algorithm was utilized indeterminate cases. Results...
ELISpot and its variant, fluorescent (FluoroSpot), are commonly used methodologies that monitor detect cytokine and/or antibody secretion from peripheral blood mononuclear cells (PBMCs). Conventional FluoroSpot assays utilize PVDF-membrane plates, which require labor-intensive washing steps may introduce variability in performance results. Here, we have developed a novel assay using polystyrene-, clear-bottom microplates with simplified automated to minimize operator error. In this work,...
Abstract To develop effective rAAV gene delivery vectors, scientists need to consider multiple elements, such as the promoter, insertion location, genetic target, and target name a few. Green fluorescent protein (GFP), or other detection markers are often used measure vector transduction expression efficiency. Transduced GFP samples can be analyzed using microscopy flow cytometry, however, both methods quite time consuming, labor intensive, lack high-throughput capabilities kinetically...
Abstract Two active double metal cyanide (DMC) complexes were successfully synthesized by solvent‐free grinding method. Their structures characterized FTIR spectrometer and X‐ray diffractometer. The results showed that Complex 1 (double complex with K 3 Fe(CN) 6 ZnCl 2 ) Zn(CH COO) had the same structures, crystal forms, lower crystallinity as both of them conventional solvent‐based methods, respectively. Investigations on conditions indicated ground 14 min at a high strength could achieve...
Most previous studies have focused on analgesic and anti-cancer activities for the conotoxins identified from piscivorous molluscivorous cone snails, but little attention has been devoted to insecticidal activity of dominant vermivorous species. As a representative snail, Chinese tubular snail (Conus betulinus) is Conus species inhabiting South China Sea. We sequenced related venom transcriptomes C. betulinus using both next-generation sequencing traditional Sanger technologies,...
Benzyl isothiocyanate (BITC) is a dietary derived from cruciferous vegetables. Recent studies showed that BITC inhibited the growth of many cancer cells, including hepatocellular carcinoma (HCC) cells. Alpha-fetoprotein (AFP) important molecule for promoting progression HCC, in present investigation, we explore influence AFP on role malignant behaviours HCC and potential underlying mechanisms. We found thatBITC viability, migration, invasion induced apoptosis human liver cell lines, Bel...