A5068284355- Advanced Electron Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Electron and X-Ray Spectroscopy Techniques
- Endoplasmic Reticulum Stress and Disease
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Rheumatoid Arthritis Research and Therapies
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Plant Stress Responses and Tolerance
- COVID-19 Clinical Research Studies
- Inhalation and Respiratory Drug Delivery
- Surface and Thin Film Phenomena
- SARS-CoV-2 and COVID-19 Research
- Protein Tyrosine Phosphatases
- Geological and Geophysical Studies
- Plant Virus Research Studies
- interferon and immune responses
- RNA regulation and disease
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
Tianjin Medical University
2021-2024
The Affiliated Hospital to Changchun University of Chinese Medicine
2017-2024
University of South China
2024
Tianjin Medical University General Hospital
2023
Purdue University West Lafayette
2013-2022
Center for Cancer Research
2021-2022
Chinese Academy of Sciences
1999-2022
Institute of Biophysics
2021-2022
University of Chinese Academy of Sciences
2021-2022
Changchun University of Chinese Medicine
2020
Analogous to the atom–molecule relationship, nanoparticle (NP) clusters (or NP-molecules) with defined compositions and directional bonds could potentially integrate properties of component individual NPs, leading emergent properties. Despite extensive efforts in this direction, no general approach is available for assembly such NP-molecules. Here we report a method building type structures by encapsulating NPs into self-assembled DNA polyhedral wireframe nanocages, which serve as guiding...
Nicotinamide adenine dinucleotide (NAD+) is a central metabolite in cellular processes. Depletion of NAD+ has been demonstrated to be prevalent theme both prokaryotic and eukaryotic immune responses. Short Argonaute proteins (Agos) are associated with NADase domain-containing (TIR-APAZ or SIR2-APAZ) encoded the same operon. They confer immunity against mobile genetic elements, such as bacteriophages plasmids, by inducing depletion upon recognition target nucleic acids. However, molecular...
Abstract The Silent Information Regulator 2 (SIR2) protein is widely implicated in antiviral response by depleting the cellular metabolite NAD + . defense-associated sirtuin (DSR2) effector, a SIR2 domain-containing protein, protects bacteria from phage infection , while an anti-DSR2 (DSR anti-defense 1, DSAD1) employed some phages to evade this host defense. NADase activity of DSR2 unleashed recognizing tail tube (TTP). However, activation and inhibition mechanisms are unclear. Here, we...
DNA offers excellent programming properties to nanomaterials syntheses. Host–guest interaction between nanostructures and inorganic nanoparticles (NPs) is of particular interest because the resulting complexes would possess both intrinsic physical associated with NPs, such as plasmonic magnetic features. Here, we report a class core–shell (AuNP@DNA cages): hard gold NPs (AuNPs) are encapsulated in geometrically well-defined soft nanocages. The AuNP guest can be further controllably released...
Current tile-based DNA self-assembly produces simple repetitive or highly symmetric structures. In the case of 2D lattices, unit cell often contains only one basic tile because tiles are (in terms either backbone sequence). this work, we have applied retrosynthetic analysis to determine minimal asymmetric units for complex nanostructures. Such guides us break intrinsic structural symmetries achieve high complexities. This strategy has led construction several nanostructures that not...
Tulane virus (TV) is a newly isolated cultivatable calicivirus that infects juvenile rhesus macaques. Here we report 6.3 Å resolution cryo-electron microscopy structure of the TV virion. The virion about 400 in diameter and consists T = 3 icosahedral protein capsid enclosing RNA genome. 180 copies major VP1 (∼57 KDa) are organized into two types dimers A/B C/C form thin, smooth shell studded with 90 dimeric protrusions. overall organization fold closely resemble other caliciviruses,...
Abstract We report the fabrication of transmission electron microscopy (TEM) grids bearing graphene oxide (GO) sheets that have been modified with N α , -dicarboxymethyllysine (NTA) and deactivating agents to block non-selective binding between GO-NTA non-target proteins. The resulting GO-NTA-coated these improved antifouling properties were then used isolate His 6 -T7 bacteriophage -GroEL directly from cell lysates. To demonstrate utility simplified workflow enabled by grids, we performed...
Tumor suppressor PTEN (phosphatase and tensin homologue deleted on chromosome 10) levels are frequently found reduced in human cancers, but how is down-regulated not fully understood. In addition, although a compelling connection exists between PRL of regenerating liver) 2 cancer, this phosphatase induces oncogenesis has been an enigma. Here, we discovered that PRL2 ablation inhibits heterozygosity-induced tumorigenesis. deficiency elevates attenuates AKT signaling, leading to decreased...
Mobile genetic elements such as phages and plasmids have evolved anti-CRISPR proteins (Acrs) to suppress CRISPR-Cas adaptive immune systems. Recently, several phage non-phage derived Acrs including AcrIIA17 AcrIIA18 been reported inhibit Cas9 through modulation of sgRNA. Here, we show that inactivate distinct mechanisms. inhibits activity interference with Cas9-sgRNA binary complex formation. In contrast, induces the truncation sgRNA in a Cas9-dependent manner, generating shortened incapable...
Abstract The type III-E CRISPR–Cas systems are newly identified adaptive immune in prokaryotes that use a single Cas7–11 protein to specifically cleave target RNA. could associate with Csx29, putative caspase-like encoded by the gene frequently found loci, suggesting functional linkage between RNase and protease activities systems. Here, we demonstrated RNA recognition would stimulate proteolytic activity of Csx30 is endogenous substrate. More interestingly, while cognate activate...
Many of the currently available COVID-19 vaccines and therapeutics are not effective against newly emerged SARS-CoV-2 variants. Here, we developed metallo-enzyme domain angiotensin converting enzyme 2 (ACE2)-the cellular receptor SARS-CoV-2-into an IgM-like inhalable molecule (HH-120). HH-120 binds to Spike (S) protein with high avidity confers potent broad-spectrum neutralization activity all known variants concern. was as inhaled formulation that achieves appropriate aerodynamic properties...
We report the preparation and performance of TEM grids bearing stabilized nonfouling lipid monolayer coatings. These films contain NTA capture ligands controllable areal density at distal end a flexible poly(ethylene glycol) 2000 (PEG2000) spacer to avoid preferred orientation surface-bound histidine-tagged (His-tag) protein targets. Langmuir–Schaefer deposition 30 mN/m mixed monolayers containing two novel synthetic...
Significance The enormous prevalence of tailed double-stranded DNA bacteriophages on this planet is made possible by highly efficient self-assembly usually hundreds protein subunits into stable capsids. These capsids must stand with an internal pressure as a result the phage DNA-packaging process. Here we report complete atomic model headful bacteriophage Sf6 at 2.9 Å resolution determined electron cryo-microscopy. structure reveals DNA-inflated, tensed state robust yet flexible shell...
Plant-associated bacteria play important regulatory roles in modulating plant hormone auxin levels, affecting the growth and yields of crops. A conserved degradation ( iad ) operon was recently identified Variovorax genomes, which is responsible for root inhibition (RGI) reversion, promoting rhizosphere colonization growth. However, molecular mechanism underlying by remains unclear. Here, we systematically screened products 2 proteins, IadK2 IadD, that directly associate with indole-3-acetic...
VCP/p97 is an evolutionarily conserved AAA+ ATPase important for cellular homeostasis. Previous studies suggest that VCP predominantly exists as a homohexamer. Here, we performed structural and biochemical characterization of dodecamer, understudied state VCP. The structure revealed apo nucleotide status has rarely been captured, tail-to-tail assembly two hexamers, the up-elevated N-terminal domains akin to seen in ATP-bound hexamer. Further analyses elucidated status-dependent...
ABSTRACTOverexpression of PTP4A phosphatases are associated with advanced cancers, but their biological functions far from fully understood due to limited knowledge about physiological substrates. VCP is implicated in lysophagy via collaboration specific cofactors the ELDR complex. However, how complex assembly regulated has not been determined. Moreover, functional significance penultimate and conserved Tyr805 phosphorylation established. Here, we use an unbiased substrate trapping mass...