A5103150696- Dermatology and Skin Diseases
- Bone Metabolism and Diseases
- Asthma and respiratory diseases
- Bone health and treatments
- Signaling Pathways in Disease
- IL-33, ST2, and ILC Pathways
- Venous Thromboembolism Diagnosis and Management
- Alkaline Phosphatase Research Studies
- Extracellular vesicles in disease
- Cardiac Fibrosis and Remodeling
- Blood transfusion and management
- Cardiac electrophysiology and arrhythmias
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Cutaneous Melanoma Detection and Management
- Immunotherapy and Immune Responses
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Immune Cell Function and Interaction
- Protein Tyrosine Phosphatases
- Wound Healing and Treatments
- Epigenetics and DNA Methylation
- Spinal Cord Injury Research
- Congenital heart defects research
- Autophagy in Disease and Therapy
Linyi People's Hospital
2018-2025
Tropical Crops Genetic Resources Institute
2025
Ministry of Agriculture and Rural Affairs
2025
Chinese Academy of Tropical Agricultural Sciences
2025
Indiana University School of Medicine
2006-2024
Indiana University
2018-2024
Wenzhou Polytechnic
2024
Shandong First Medical University
2023-2024
Indiana University – Purdue University Indianapolis
2006-2024
Wenzhou University
2024
All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are released from membrane by proteases. In several instances, ectodomain release is critical for activation EGFR ligands, highlighting importance identifying ligand sheddases. Here, we uncovered sheddases six using mouse embryonic cells lacking candidate-releasing enzymes (a disintegrin metalloprotease [ADAM] 9, 10, 12, 15, 17, 19). ADAM10 emerged as main sheddase EGF...
Abstract Background As important players in cell-to-cell communication, exosomes (exo) are believed to play a similar role promoting fracture healing. This study investigated whether derived from bone marrow mesenchymal stem cells (BMMSC-Exos) could improve healing of nonunion. Methods BMMSC-Exos were isolated and transplanted into the site rat model femoral nonunion (Exo group) every week. Moreover, equal volumes phosphate-buffered saline (PBS) exosome-depleted conditioned medium (CM-Exo)...
Hip osteoarthritis is a common disabling condition of the hip joint and associated with substantial health burden. We assessed epidemiological patterns from 1990 to 2019 by sex, age, socio-demographic index (SDI).Age-standardized rates (ASRs) were obtained for incidence disability-adjusted life years (DALYs) 21 regions, encompassing total 204 countries territories. The estimated annual percentage changes (EAPCs) ASRs calculated evaluate trends in DALYs over these 30 years.Globally, 2019,...
Severe spinal cord injury (SCI) is caused by external mechanical injury, resulting in unrecoverable neurological injury. Recent studies have shown that exosomes derived from bone marrow mesenchymal stem cells (BMSCs-Exos) might be valuable paracrine molecules the treatment of SCI. In this study, we designed SCI models vivo and vitro then investigated possible mechanism successful repair BMSCs-Exos. vivo, established one Sham group two model groups. The Basso, Beattie, Bresnahan (BBB) scores...
Asthma is a chronic inflammatory lung disease with intermittent flares predominately mediated through memory T cells. Yet, the identity of long-term cells that mediate allergic recall responses not well defined. In this report, using mouse model allergen exposure followed by an allergen-free rest period, we characterized subpopulation CD4+ secreted IL-9 as obligate effector cytokine. IL-9-secreting had resident cell phenotype, and blocking during challenge or deleting from significantly...
The imbalance between osteoblasts and osteoclasts may lead to osteoporosis. Osteoblasts have different energy requirements, with aerobic glycolysis being the prominent metabolic feature of osteoblasts, while osteoclast differentiation fusion are driven by oxidative phosphorylation.By polymerase chain reaction as well Western blotting, we assayed coactivator-associated arginine methyltransferase 1 (CARM1) expression in bone tissue, mouse precranial osteoblast cell line MC3T3-E1 monocyte...
Both agonist studies and loss-of-function models indicate that PPARγ plays an important role in cutaneous biology. Since has a high level of basal activity, we hypothesized epidermal would regulate normal homeostatic processes within the epidermis. In this current study, performed mRNA sequencing differential expression analysis scrapings from knockout mice wildtype littermates. Pparg-/-epi exhibited 1.5-fold or greater change 11.8% 14,482 identified transcripts. Up-regulated transcripts...
Glucocorticoids are used for the treatment of inflammatory diseases, but glucocorticoid is associated with bone damage. Resveratrol a phytoalexin found in many plants, and we investigated its protective role on dexamethasone-induced dysfunction MC3T3-E1 cells primary osteoblasts.MC3T3-E1 osteoblasts were treated dexamethasone presence/absence different doses resveratrol 24 or 48 h. Then, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium (MTT) lactate dehydrogenase (LDH) assays to evaluate...
ABSTRACTOverexpression of PTP4A phosphatases are associated with advanced cancers, but their biological functions far from fully understood due to limited knowledge about physiological substrates. VCP is implicated in lysophagy via collaboration specific cofactors the ELDR complex. However, how complex assembly regulated has not been determined. Moreover, functional significance penultimate and conserved Tyr805 phosphorylation established. Here, we use an unbiased substrate trapping mass...
Silicon, an element, has been reported to possess various osteogenic biological functions. However, the precise cellular action mechanism of silicon in osteogenesis is elusive. Here, we examine whether autophagy associated with differentiation and mineralization preosteoblasts stimulated by orthosilicic acid (unique soluble form silicon). The induced was detected via autophagy-related protein expression. correlation between acid-induced process verified inhibitor 3-methyladenine. We also...
The Na+-Ca2+ exchanger (NCX1) is up-regulated in hypertrophy and often found end-stage heart failure. Studies have shown that the change its expression contributes to contractile dysfunction. We previously 1831-bp Ncx1 H1 (1831Ncx1) promoter directs cardiac-specific of both development adult, sufficient for up-regulation response pressure overload. Here, we utilized adenoviral mediated gene transfer transgenics identify minimal regions elements mediate heart. demonstrate proximal 184 bp...
Congenital hydrocephalus is a common birth-defect whose developmental origins are poorly understood. Pax3-null mutants show defects in myogenesis, neural tube closure, crest morphogenesis, and heart development that, consequently, results embryonic lethality. Here we demonstrate that conditional deletion of the mouse Pax3 transcription factor fully-penetrant congenital obstructive hydrocephalus. To identify role during cranial development, deleted within neuroepithelium (via Pax7−Cre),...
Neural cell apoptosis serves a key role in spinal cord injury (SCI), which is threat to human health. The present study aimed evaluate the neuroprotective mechanism of salvianolic acid B (Sal B) (SCI) rat model. Basso, Beattie, and Bresnahan scores demonstrated that Sal treatment significantly increased locomotor functional recovery SCI rats compared with model between 3 8 weeks. Nissl staining enhanced motor neuron survival decreased lesion size after SCI. Reverse transcription-quantitative...
Background and Objective:In 2023, an estimated 97,610 melanomas will be diagnosed in the U.S. Immunotherapy has revolutionized care for melanoma patients highlighted prognostic value of characterizing immune infiltrates tissue. This project used spatial transcriptomics (ST) to analyze cutaneous specimens. Methods:Visium Spatial Gene Expression platform generated datasets from formalin fixed paraffin embedded specimens were analyzed using Loupe Browser software v6.5.0 (10x Genomics Inc.)....