A5038459784- Glycosylation and Glycoproteins Research
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Signaling Pathways in Disease
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Electron and X-Ray Spectroscopy Techniques
- Trace Elements in Health
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Connective tissue disorders research
- Cell Adhesion Molecules Research
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Ion channel regulation and function
- Thyroid Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Cardiac electrophysiology and arrhythmias
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
Southern University of Science and Technology
2022-2025
Shenzhen Second People's Hospital
2022
Abstract Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, homogeneity thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one main challenges for high-resolution cryo-EM. Here we found uniformity relies on surface flatness supporting film, developed method use ultraflat graphene (UFG) as support cryo-EM achieve...
Abstract Alpha-fetoprotein (AFP), a serum glycoprotein, is expressed during embryonic development and the pathogenesis of liver cancer. It serves as clinical tumor marker, function carcinogen, immune suppressor, transport vehicle; but detailed AFP structural information has not yet been reported. In this study, we used single-particle cryo-electron microscopy(cryo-EM) to analyze structure recombinant obtained 3.31 Å cryo-EM built an atomic model AFP. We observed identified certain features...
Adenosine deaminase acting on RNA (ADAR) proteins, which mediate adenosine-to-inosine editing of double-stranded ribonucleic acid (dsRNA) substrates, play essential roles in balancing innate immunity. Using cryogenic electron microscopy, we solved the structure Caenorhabditis elegans ADR-2-ADBP-1 complex (stoichiometric ratio, 2:2), is an asymmetric ADR-2 dimer with one site blocked by other ADR-2. Unexpectedly, dsRNA recruitment triggered dissociation dimer, exposing more competent sites....
Abstract Secretory-pathway Ca 2+ -ATPases (SPCAs) play critical roles in maintaining homeostasis, but the exact mechanism of SPCAs-mediated transport remains unclear. Here, we determined six cryo-electron microscopy (cryo-EM) structures human SPCA1 (hSPCA1) a series intermediate states, revealing near-complete conformational cycle. With aid molecular dynamics simulations, these offer clear structural basis for entry and release hSPCA1. We found that hSPCA1 undergoes unique changes during ATP...
Abstract Human multidrug resistance protein 5 (hMRP5) effluxes anticancer and antivirus drugs, driving resistance. To uncover the mechanism of hMRP5, we determine six distinct cryo-EM structures, revealing an autoinhibitory N-terminal peptide that must dissociate to permit subsequent substrate recruitment. Guided by these molecular insights, design inhibitory could block entry into transport pathway. We also identify a regulatory motif, comprising positively charged cluster hydrophobic...
Calcium/calmodulin‐dependent serine protein kinase (CASK) interaction 1/2 (Caskin1/2) is essential neuronal synaptic scaffold in nervous system development. Knockouts of Caskin1/2 display severe deficits novelty recognition and spatial memory. The tandem sterile alpha motif (SAM) domains Caskin1/2, also conserved their Drosophila homolog Ckn, are known to form homopolymers, yet dynamic regulation mechanism remains unclear. In this study, SAM SH3 domain‐containing 1 (SASH1) was first...