A5038524016- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Probiotics and Fermented Foods
- Machine Learning in Bioinformatics
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- Advanced Mathematical Identities
- Mathematical functions and polynomials
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Metal complexes synthesis and properties
- Cellular transport and secretion
- Glycogen Storage Diseases and Myoclonus
- Advanced Combinatorial Mathematics
- X-ray Diffraction in Crystallography
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Crystal structures of chemical compounds
- DNA and Nucleic Acid Chemistry
- Protein Degradation and Inhibitors
- Leaf Properties and Growth Measurement
- Bacteriophages and microbial interactions
University of Hong Kong
2018-2025
Hong Kong University of Science and Technology
2018-2025
National Institutes of Health
2023-2024
Stanford University
2022-2024
National Institute of Neurological Disorders and Stroke
2023-2024
Stanford Synchrotron Radiation Lightsource
2023-2024
Hunan University of Science and Engineering
2024
Chinese University of Hong Kong
2023-2024
Pennsylvania State University
2023-2024
Henan Polytechnic University
2019-2022
Although blocking the binding of vascular endothelial growth factor (VEGF) to neuropilin-2 (NRP2) on tumor cells is a potential strategy treat aggressive carcinomas, lack effective reagents that can be used clinically has hampered this therapy. Here, we describe generation fully humanized, high-affinity monoclonal antibody (aNRP2-10) specifically inhibits VEGF NRP2, conferring antitumor activity without causing toxicity. Using triple-negative breast cancer as model, demonstrated aNRP2-10...
Transcription factors (TFs) control numerous genes that are directly relevant to many human disorders. However, developing specific reagents targeting TFs within intact cells is challenging due the presence of highly disordered regions these proteins. Intracellular antibodies offer opportunities probe protein function and validate therapeutic targets. Here, we describe optimization nanobodies for BCL11A, a validated target treatment hemoglobin We obtained first-generation directed region...
Proximity-based strategies to degrade proteins have enormous therapeutic potential in medicine, but the technologies are limited for which small molecule ligands exist. The identification of such therapeutically relevant "undruggable" remains challenging. Herein, we employed yeast surface display synthetic nanobodies identify a protein ligand selective BCL11A, critical repressor fetal globin gene transcription. Fusion nanobody cell-permeant miniature and an E3 adaptor creates degrader that...
The tRNA synthetases catalyze the first step of protein synthesis and have increasingly been studied for their nuclear extra-cellular ex-translational activities. Human genetic conditions such as Charcot-Marie-Tooth attributed to dominant gain-of-function mutations in some synthetases. Unlike dominantly inherited mutations, recessive loss-of-function can potentially elucidate We present here five individuals from four families with a multi-system disease associated bi-allelic FARSB that...
Abstract Aminoacyl-tRNA synthetases (ARSs) function to transfer amino acids cognate tRNA molecules, which are required for protein translation. To date, biallelic mutations in 31 ARS genes known cause recessive, early-onset severe multi-organ diseases. VARS encodes the only valine cytoplasmic-localized aminoacyl-tRNA synthetase. Here, we report seven patients from five unrelated families with different missense variants . Subjects present a range of global developmental delay, epileptic...
Abstract Glycogen is a branched glucose polymer and serves as an important energy store. Its debranching critical step in its mobilization. In animals fungi, the 170 kDa glycogen enzyme (GDE) catalyses this reaction. GDE deficiencies humans are associated with severe diseases collectively termed storage disease type III (GSDIII). We report crystal structures of complex oligosaccharides, structure-guided mutagenesis biochemical studies to assess structural observations. These reveal that...
Interstitial lung disease (ILD) consists of a group immune-mediated disorders that can cause inflammation and progressive fibrosis the lungs, representing an area unmet medical need given lack disease-modifying therapies toxicities associated with current treatment options. Tissue-specific splice variants (SVs) human aminoacyl-tRNA synthetases (aaRSs) are catalytic nulls thought to confer regulatory functions. One example from histidyl-tRNA synthetase (HARS), termed HARS WHEP because...
Abstract To overcome the challenge of balancing imaging speecd and resolution, which currently limits accurate identification structural dynamic changes in study endoplasmic reticulum (ER) plant cells. This research employs structured illumination microscopy techniques to achieve super-resolution real-time ER live Arabidopsis materials. Additionally, a self-supervised denoising framework (Blind2Unblind) was optimized further enhance signal-to-noise ratio rapid microscopic imaging. Based on...
Abstract The yeast protein Rad5 and its orthologs in other eukaryotes promote replication stress tolerance cell survival using their multiple activities, including ubiquitin ligase, fork remodeling DNA lesion targeting activities. Here, we present the crystal structure of a nearly full-length protein. shows three distinct, but well-connected, domains required for Rad5’s spatial arrangement these suggest that different can have autonomous activities also undergo intrinsic coordination....
Remote sensing estimation of crop nitrogen content allows real-time monitoring growth to develop scientific methods. However, most the current remote estimates contents have limitations in accurately reflecting vertical distribution nutrients plants. Firstly, original hyperspectrum is first-order differential (FD), second-order (SD), and continuous removal (CR), corresponding sensitive bands were screened by correlation analysis this paper. Then, spectral reflectance, vegetation indices,...
Journal Article A splicing mutation in the COL7A1 gene causes autosomal dominant dystrophic epidermolysis bullosa pruriginosa Get access X. Ren, Ren Center for Human Genome Research and College of Life Science Technology, Huazhong University Wuhan, Hubei 430074, China Search other works by this author on: Oxford Academic Google Scholar J.Y. Liu, Liu L.Y. Zhai, Zhai Department Dermatology, 1st People's Hospital Nanyang, Henan 473000, Q. Yao, Yao Dai, Dai Z. Cai, Cai P. K. Sun, Sun C. Huang,...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Abstract C 13 H 10 N 2 S, orthorhombic, Pccn (no. 56), a = 30.55(2) Å, b 7.760(6) c 9.622(7) V 2,281(3) Å 3 , Z 4, R gt ( F ) 0.0393, wR ref 0.1033, T 296(2) K.
Bacterial acquisition of metabolites is largely facilitated by transporters with unique substrate scopes. The tripartite ATP-independent periplasmic (TRAP) comprise a large family bacterial proteins that facilitate the uptake variety small molecules. It has been reported some TRAP systems encode fourth protein, T component. T-component, or TatT, predicted to be periplasmic-facing lipoprotein enables from outer membrane. However, no substrates were revealed for any TatT and their functional...