A5041164215- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Steroid Chemistry and Biochemistry
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Machine Learning in Bioinformatics
- Pharmacogenetics and Drug Metabolism
- Cholesterol and Lipid Metabolism
- Tuberculosis Research and Epidemiology
- Computational Drug Discovery Methods
- Enzyme Production and Characterization
- Pharmacological Effects of Natural Compounds
Pennsylvania State University
2023-2024
Stanford University
2022-2024
National Institute of Neurological Disorders and Stroke
2023-2024
National Institutes of Health
2023-2024
Stanford Synchrotron Radiation Lightsource
2023-2024
Stony Brook University
2015-2020
The metabolism of host cholesterol by Mycobacterium tuberculosis (Mtb) is an important factor for both its virulence and pathogenesis, although how why required not fully understood. Mtb uses a unique set catabolic enzymes that are homologous to those classical β-oxidation fatty acids but specific steroid-derived substrates. Here, we identify assign the substrate specificities two these enzymes, ChsE4-ChsE5 (Rv3504-Rv3505) ChsE3 (Rv3573c), carry out side chain oxidation in Mtb. Steady-state...
Cholesterol is a major carbon source for Mycobacterium tuberculosis (Mtb) during infection, and cholesterol utilization plays significant role in persistence virulence within host macrophages. Elucidating the mechanism by which degraded may permit identification of new therapeutic targets. Here, we characterized EchA19 (Rv3516), an enoyl-CoA hydratase involved side-chain catabolism. Steady-state kinetics assays demonstrated that preferentially hydrates metabolite...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...
Abstract Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4...
Sterol lipids are widely present in eukaryotes and play essential roles signaling modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function is not known. Moreover, many more species, including pathogens commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize producing a mixture of C-4 methylated...