A5041453699- Steroid Chemistry and Biochemistry
- Enzyme Structure and Function
- Pharmacogenetics and Drug Metabolism
- Polymer-Based Agricultural Enhancements
- Pesticide and Herbicide Environmental Studies
- Plant Surface Properties and Treatments
- Biochemical and Molecular Research
- Tuberculosis Research and Epidemiology
- Pharmacological Effects of Natural Compounds
- Cancer-related Molecular Pathways
- Mycobacterium research and diagnosis
- Protein Kinase Regulation and GTPase Signaling
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Surfactants and Colloidal Systems
- Drug Solubulity and Delivery Systems
- Renal cell carcinoma treatment
- Synthesis and Catalytic Reactions
- Microbial metabolism and enzyme function
- Advanced Breast Cancer Therapies
- Advanced Drug Delivery Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Topology and Set Theory
- Extracellular vesicles in disease
- Drug Transport and Resistance Mechanisms
China Pharmaceutical University
2006-2024
Nanjing Drum Tower Hospital
2024
Stony Brook University
2012-2020
Harbin Institute of Technology
1988-2016
University of California, Irvine Medical Center
1989
Abstract Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning diameter range 500 to 3000 nm. They are emitted by migrating cells and harbour diverse array RNAs proteins. can be readily identified in bodily fluids like serum urine, rendering them valuable non‐invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce streamlined effective approach the capture quantitative assessment migrasomes, employing wheat germ...
DNA probes (Gen-Probe, San Diego, Calif.) directed at the Mycobacterium tuberculosis complex and avium-M. intracellulare were used to identify acid-fast bacilli directly from specimens grown in BACTEC 12B bottles (Becton Dickinson Co., Towson, Md.). Clinical inoculated or after decontamination into a bottle, Middlebrook 7H11 agar, Lowenstein-Jensen medium. Conventional media incubated 37 degrees C 5% CO2 examined weekly for 6 weeks. Identification of isolates on conventional by standard...
The metabolism of host cholesterol by Mycobacterium tuberculosis (Mtb) is an important factor for both its virulence and pathogenesis, although how why required not fully understood. Mtb uses a unique set catabolic enzymes that are homologous to those classical β-oxidation fatty acids but specific steroid-derived substrates. Here, we identify assign the substrate specificities two these enzymes, ChsE4-ChsE5 (Rv3504-Rv3505) ChsE3 (Rv3573c), carry out side chain oxidation in Mtb. Steady-state...
By means of high-speed emulsification and ultrasonic dispersing, emamectin benzoate (EB)–sodium lignosulfonate (SL) nanoformulation (EBSLNf) with a particle size 150–250 nm was developed by the electrostatic self-assembly SL EB. The morphology characterized UV, IR, dynamic light scattering, fluorescence spectra (FS), scanning electron microscopy, transmission microscopy. results antiphotolysis controlled release test for EBSLNf showed that ability is four times higher than commercial...
It is extremely important to develop a facile and versatile strategy for effectively separating various oil–water mixtures. Here we report that the pith of juncus (which also known as common rushes) can serve material separation. Under action capillary force gravity, piece automatically continuously separates not only immiscible mixtures but surfactant-stabilized water-in-oil emulsions with high selectivity desirable flux. The separation cost-effective energy-efficient because it avoids...
Mycobacterium tuberculosis (Mtb), the causative agent of (TB), is a highly successful human pathogen and has infected approximately one-third world's population. Multiple drug resistant (MDR) extensively (XDR) TB strains coinfection with HIV have increased challenges successfully treating this disease pandemic. The metabolism host cholesterol by Mtb an important factor for both its virulence pathogenesis. In Mtb, side chain degraded through multiple cycles β-oxidation FadA5 (Rv3546)...
Cholesterol is a major carbon source for Mycobacterium tuberculosis (Mtb) during infection, and cholesterol utilization plays significant role in persistence virulence within host macrophages. Elucidating the mechanism by which degraded may permit identification of new therapeutic targets. Here, we characterized EchA19 (Rv3516), an enoyl-CoA hydratase involved side-chain catabolism. Steady-state kinetics assays demonstrated that preferentially hydrates metabolite...
Using beeswax as wrapping matrix, two types of release-controlled TM (thiamethoxam)/BK(beeswax-kaolin) microcapsules were prepared by adsorbing on kaolin and then encapsulated with beeswax, or directly beeswax. The structure morphology the TM/BK characterized. effects different preparation methods, particle size, pH conditions additives release property investigated in water soil column to compare advantages approaches. Finally, insecticidal effect against sugarcane borer rice planthopper...
Metabolism of cholesterol by Mycobacterium tuberculosis (Mtb) contributes to its pathogenesis. We show that ChsE4-ChsE5 (Rv3504/Rv3505) specifically catalyzes dehydrogenation the (25S)-3-oxo-cholest-4-en-26-oyl-CoA diastereomer in side chain β-oxidation. Thus, a dichotomy between supply both 25R and 25S metabolic precursors upstream cytochrome P450s substrate stereospecificity exists. reconcile dilemma metabolite production demonstrating mycobacterial MCR (Rv1143) can efficiently epimerize...
Abstract Background: The pathogenesis of diabetic nephropathy (DN) is complex, and its onset occult. It should be considered from multiple levels angles using the concept "system biology". New technologies, such as omics high-throughput screening, provide more strategies for screening ideal markers DN. Methods: we used data-independent acquisition (DIA) mass spectrometry urine proteomic analysis DN patients at different pathological stages healthy controls. Results:...
New drugs with novel mechanisms of action are required to meet the severe threat human health posed by emergence drug resistant strains M. tuberculosis (M. tb). tb imports and metabolizes host cholesterol during infection, which is critical for maintenance infection. Both igr operon fadA5 in vitro growth using as a sole carbon source full virulence mouse model vivo We have elucidated function these genes, their corresponding enzymes role metabolism combination transcriptional profiling,...
Using polyethylene wax (PW) as the coating matrix, lambda-cyhalothrin-PW nanosuspoemulsion (LC-PW) with a particle size of 80-150nm was prepared through high-speed stirring, hot melt emulsification and ultrasonic dispersion. The formulation composition LC-PW were optimized, morphology analyzed by dynamic light scattering (DLS) TEM, structure characterized UV IR. anti-photolysis test showed that had good performance. Furthermore, could sustainably release Lambda-cyhalothrin, which pH-...
AK4 has been validated as a crucial effector of various signal pathways and play an important role in driving tumor progression. Here, we developed series 7H-pyrrolo [2,3-d] pyrimidine derivatives PAK4 inhibitors. Compounds 5n 5o showed higher enzymatic inhibitory activities (IC50 = 2.7 20.2 nM, respectively) potent activity 7.8 38.3 against MV4-11 cell line. Further flow cytometry assay revealed that the compound can arrest cells at G0/G1 phase induce apoptosis. Molecular mechanism study...