A5083599087- RNA Research and Splicing
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- Innovative Teaching Methods
- Science Education and Pedagogy
- Educational Strategies and Epistemologies
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Mobile Learning in Education
- Career Development and Diversity
- Plant Virus Research Studies
- Various Chemistry Research Topics
- Education and Critical Thinking Development
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Biomedical and Engineering Education
- Nuclear Physics and Applications
- Field-Flow Fractionation Techniques
- Visual and Cognitive Learning Processes
- Virus-based gene therapy research
- Plant and Fungal Interactions Research
- Renal function and acid-base balance
- QR Code Applications and Technologies
University of North Georgia
2013-2023
College of New Jersey
2007
Johns Hopkins University
2001-2005
Hebrew University of Jerusalem
2003-2005
Universidad Metropolitana
2004-2005
Johns Hopkins Medicine
2001-2003
Cornell University
2003
Nesprin‐1α is a spectrin repeat (SR)‐containing, transmembrane protein of the inner nuclear membrane, and highly expressed in muscle cells. A yeast two‐hybrid screen for nesprin‐1α‐interacting proteins showed that nesprin‐1α interacted with itself. Blot overlay experiments revealed nesprin‐1α's third SR binds fifth SR. The carboxy‐terminal half directly bound lamin A, intermediate filament protein. Biochemical analysis demonstrated dimers bind to nucleoplasmic domain emerin, an membrane...
Mutations in emerin cause the X-linked recessive form of Emery-Dreifuss muscular dystrophy (EDMD). Emerin localizes at inner membrane nuclear envelope (NE) during interphase, and diffuses into ER when NE disassembles mitosis. We analyzed recruitment wildtype mutant GFP-tagged proteins assembly living HeLa cells. During telophase, accumulates briefly ‘core’ region telophase chromosomes, later distributes over entire rim. Barrier-to-autointegration factor (BAF), a protein that binds...
Emerin and MAN1 are LEM domain-containing integral membrane proteins of the vertebrate nuclear envelope. The function is unknown, whereas emerin known to interact with lamins, barrier-to-autointegration factor (BAF), nesprin-1α, a transcription repressor. Mutations in cause X-linked recessive Emery–Dreifuss muscular dystrophy. homologs both conserved Caenorhabditis elegans , but loss Ce-emerin has no detectable phenotype. We therefore used C. test hypothesis that Ce-MAN1 overlaps...
Barrier-to-autointegration factor (BAF) is a DNA-bridging protein, highly conserved in metazoans. BAF binds directly to LEM (LAP2, emerin, MAN1) domain nuclear membrane proteins, including LAP2 and emerin. We used site-directed mutagenesis biochemical analysis map functionally important residues human BAF, those required for direct binding DNA or also tested wild-type 25 point mutants their effects on assembly Xenopus egg extracts, which contain ∼12 μM endogenous dimers. Exogenous caused two...
Barrier-to-autointegration factor (BAF) binds dsDNA, LEM-domain proteins, and lamins. Caenorhabditis elegans BAF requires Ce-lamin two proteins (Ce-emerin Ce-MAN1) to localize during nuclear assembly. It was unknown whether LEM in turn, depend on Ce-BAF (mutually dependent structural roles). RNA interference-mediated down-regulation of caused gross defects chromosome segregation, chromatin decondensation, mitotic progression as early the two-cell stage, embryos died at ≈100-cell stage....
Student engagement in the analysis of primary scientific literature increases critical thinking, literacy, data evaluation, and science process skills. However, little is known about by which expertise reading articles develops. For this reason, we decided to compare how faculty experts student novices engage with a research article. We performed think-aloud interviews biology undergraduates as they read through analyzed these using qualitative methods. grounded interpretation cognitive load...
Analysis of the primary literature in undergraduate curriculum is associated with gains student learning. In particular, CREATE (Consider, Read, Elucidate hypotheses, Analyze and interpret data, Think next Experiment) method an increase critical thinking skills. We adapted within a required cell biology class compared learning students using to those involved less structured discussions. found that while both sets had thinking, who used did not show significant improvement over engaged more...
Reading primary literature is a popular classroom practice that exposes students to the process of science. However, analysis can be taxing and time-consuming for students. For this reason, it important determine source student challenges what motivates them read literature. To better understand students’ challenges, preferences, motivations towards analyzing literature, we held focus groups with biology undergraduates where asked about their thoughts perceptions on practice. Students felt...
Loss of emerin, a nuclear membrane protein, causes Emery-Dreifuss muscular dystrophy (EDMD), characterized by muscle weakening, contractures major tendons and potentially lethal cardiac conduction system defects. Emerin has LEM-domain therefore binds barrier-to-autointegration factor (BAF), conserved chromatin protein essential for cell division. BAF recruits emerin to regulates higher-order structure during assembly. also filaments formed A-type lamins, mutations in which cause EDMD. Other...
As the amount and complexity of scientific knowledge continues to grow, it is essential educate scientifically literate citizens who can comprehend process science implications technological advances. This especially important when educating science, technology, engineering, mathematics (STEM) college students, since they may play a central role in future research its communication. A part decoding interpreting information ability analyze articles. For this reason, many different approaches...
The impact of the Internet on education has been recognized for decades, and as technology advances, ways in which students can access content is ever increasing. Most have some kind portable smart device with they without locational constraints a desktop computer. This mobility prompted abundant literature suggesting that Quick Response Codes (QR codes), two dimensional barcode, could be used to advance student learning. However, very few studies tested usefulness QR codes undergraduate...
The tragic murder of Mr. George Floyd brought to the head long-standing issues racial justice and equity in United States beyond. This prompted many institutions higher education, including professional organizations societies, engage long-overdue conversations about role scientific perpetuating racism. Similar societies organizations, Society for Advancement Biology Education Research (SABER), a leading international organization discipline-based biology education researchers, has long...
Primary Scientific Literature (PSL) has been used in undergraduate classrooms as a way to engage students with the research process and increase science literacy. Most curricula lack any formal training for undergraduates critically read PSL even though most courses require at some level. In addition, there are limited studies exploring by which expertise reading develops undergraduates. this study, we adapted behaviors that expert novice readers exhibit into quantitative assessment tool,...
Here, we describe LilHazelnut, a novel mycobacteriophage that infects Mycobacterium smegmatis mc2155. LilHazelnut is cluster Q phage shares 99% nucleotide identity with Giles, 53,746 bp in length, and has G+C content of 67.5%. temperate Siphoviridae virus, as typical family members.
We report the discovery of novel bacteriophage Donny, a Siphoviridae virus that infects Mycobacterium smegmatis mc2155. Donny has genome length 69,691 bp and G+C content 68.5%. shares 99% 93% nucleotide identity with bacteriophages Acadian Baee, respectively.
The multiple-solvent crystal structure (MSCS) approach uses high concentrations of organic solvents to characterize the interactions and effects on proteins. Here, method has been further developed an MSCS data-handling pipeline is presented that Detection Related Solvent Positions ( DRoP ) program improve data quality. used selectively model conserved water molecules, so advanced stage structural refinement reached quickly. This allows placement molecules more accurately convergence...
Mycobacteriophage CrystalP is a newly isolated phage infecting Mycobacterium smegmatis strain mc2155. has 76,483-bp genome and predicted to contain 143 protein-coding 2 tRNA genes, including repressor integrase genes consistent with temperate lifestyle. related the mycobacteriophages Toto Kostya other Cluster E phages.
Novel mycobacteriophage XianYue was isolated in Northeast Georgia and infects Mycobacteria smegmatis mc 2 155. Actinobacteriophages which share at least 50% nucleotide identity are grouped into clusters, with cluster A2. Its genome is 52,907 bp 91 open reading frames (ORFs) 62.9% GC content, it shares 86.51% Trixie.
Barrier-to-autointegration factor (BAF) is a nuclear protein conserved in metazoans. BAF necessary for assembly, chromatin organization, and gene expression. forms dimers that bind to the LEM domain proteins of inner membrane. Although several cellular functions are known, some binding sites on surface dimer not well defined. We interested determining regions involved contacting other proteins. The results from our studies will aid understanding interactions at molecular level. This help...