A5110620168- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Genetic and Kidney Cyst Diseases
- Career Development and Diversity
- Glycosylation and Glycoproteins Research
- 14-3-3 protein interactions
- Bacteriophages and microbial interactions
- Biomedical and Engineering Education
- Monoclonal and Polyclonal Antibodies Research
- Interdisciplinary Research and Collaboration
- T-cell and B-cell Immunology
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Skin and Cellular Biology Research
- Cancer Treatment and Pharmacology
- Higher Education Research Studies
- Animal Genetics and Reproduction
- Methane Hydrates and Related Phenomena
- Marine and coastal plant biology
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Protein Tyrosine Phosphatases
- Silk-based biomaterials and applications
- Cardiomyopathy and Myosin Studies
Howard Hughes Medical Institute
2013-2024
Towson University
2024
Research Experiences for Undergraduates
2024
Virginia Tech
2024
Radford University
2024
Concord University
2024
Trinity Washington University
2024
James Madison University
2016
Harvey Mudd College
2004-2009
J. Craig Venter Institute
2006
The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline soma functions that are embodied by distinct nuclei within single cell. germline-like micronucleus (MIC) its genome held in reserve sexual reproduction. soma-like macronucleus (MAC), which possesses processed from of the MIC, center gene expression does not directly contribute DNA to progeny. We report here shotgun sequencing, assembly, analysis...
We have probed the relationship between tubulin posttranslational modification and microtubule stability, using a variation of antibody-blocking technique. In human retinoblastoma cells we find that acetylated detyrosinated microtubules represent congruent subsets cells' total microtubules. also stable defined as those had not undergone polymerization within 1 h after injection biotin-tubulin were all posttranslationally modified; furthermore dynamic unmodified. therefore conclude in these...
The bacteriophage population is large, dynamic, ancient, and genetically diverse. Limited genomic information shows that phage genomes are mosaic, the genetic architecture of populations remains ill-defined. To understand structure phages infecting a single host strain, we isolated, sequenced, compared 627 Mycobacterium smegmatis. Their diversity considerable, there 28 distinct types (clusters) with related nucleotide sequences. However, amino acid sequence comparisons show pervasive...
Significance The Science Education Alliance–Phage Hunters Advancing Genomics and Evolutionary program is an inclusive Research Community with centralized programmatic scientific support, in which broad student engagement authentic science linked to increased accessibility research experiences for students; persistence of these students science, technology, engineering, mathematics; productivity faculty alike.
Abstract Background Ichthyophthirius multifiliis , commonly known as Ich, is a highly pathogenic ciliate responsible for 'white spot', disease causing significant economic losses to the global aquaculture industry. Options control are extremely limited, and Ich's obligate parasitic lifestyle makes experimental studies challenging. Unlike most well-studied protozoan parasites, Ich belongs phylum composed primarily of free-living members. Indeed, it closely related model organism Tetrahymena...
Transcripts approximately 14.5 kilobases in length from 14 different genes that encode for dynein heavy chains have been identified poly(A)+ RNA sea urchin embryos. Analysis of the changes level these transcripts response to deciliation, together with their sequence relatedness, suggests 11 or more isoforms participate regeneration external cilia on embryo, whereas single gene whose deduced closely resembles cytoplasmic other organisms appears not be involved this regeneration. The four...
Syk (p72syk) is a 72-kDa, nonreceptor, protein-tyrosine kinase that becomes tyrosine-phosphorylated and activated in B lymphocytes following aggregation of the B-cell antigen receptor. To explore subcellular location Syk, anti-IgM-activated B-cells were fractionated into soluble particulate fractions by ultracentrifugation. Activated was found predominantly fraction not associated with components Similarly, forms its homolog, ZAP-70, prepared from pervanadate-treated Jurkat T-cells. A 54-kDa...
Abstract The cross-linking of the B cell Ag receptor (BCR) is coupled to stimulation multiple intracellular signal transduction cascades via receptor-associated, protein tyrosine kinases both Src and Syk families. To monitor changes in subcellular distribution cells responding BCR cross-linking, we expressed Syk-deficient DT40 a fusion consisting green fluorescent protein. Treatment these with anti-IgM Abs leads recruitment kinase from cytoplasmic nuclear compartments site cross-linked at...
Three monoclonal antibodies specific for tubulin were tested by indirect immunofluorescence their ability to stain cytoplasmic microtubules of mouse and human fibroblastic cells. We used double label compare the staining patterns these with total microtubule complex in same cells that stained a polyclonal rabbit antitubulin reagent. Two bound all but Ab 1-6. 1 only subset within individual fixed Differential observed under various fixation conditions protocols, detergent-extracted...
Purified preparations of 10-nm neurofilaments from rat spinal cord and bovine or porcine brain contain a predominant 68,000-dalton polypeptide. This polypeptide is also major component the that copurify with tubulin isolated by cycles polymerization depolymerization. A protein has same isoelectric point molecular weight as neurofilament-associated been identified cytoskeletal in variety avian mammalian cell types, including baby hamster kidney (BHK-21) mouse 3T3, Novikoff hepatoma, chicken...
Effective mentorship is critical to the success of trainees in research career pathways, significantly impacting their productivity, academic and self-efficacy, satisfaction. Research faculty may be unaware or unprepared address mentor-mentee dynamics mentoring relationships, especially those that go beyond traditional scientific skill development. Addressing can even more challenging for mentors from well-represented backgrounds working with mentees historically excluded racial/ethnic...
Abstract Two monoclonal antibodies reactive for α‐tubulin but not β‐tubulin have been prepared, characterized in terms of their relative binding to tubulins from differnt sources by a solid‐phase assay, immunoautoradiography, and indirect immunofluorescence, utilized study flagellar motility. Our results demonstrate that α‐tubulins different species, even tissues the same are nonidentical. Especially interesting was observation one antibodies, Ab2, immunofluorescently stained microtubules...
A monoclonal antibody (mAb 7-1.1) was produced against a bovine brain microtubule-associated protein (MAP) preparation that had been separated from tubulin after initial purification by cycles of microtubule assembly and disassembly in vitro. The reacted specifically with two high molecular weight polypeptides the MAP 1 class, designated 1.1 1.2, also surfaces 1-containing microtubules assembled Double immunofluorescence microscopy using mAb 7-1.1 well-characterized rabbit anti-tubulin...
Science excellence depends on our ability to harness the powerful potential of nation's diverse talent pool. A scientific community that actively seeks full participation persons from all backgrounds and perspectives is better positioned find innovative solutions complex problems; more difficult problem, greater benefit diversity in finding solution (1). While has many different facets, dimension race ethnicity stands out because persistent racial ethnic disparities society science.
ABSTRACT Syk is a protein-tyrosine kinase that essential for B-lymphocyte development and B-cell signaling. phosphorylates tubulin on tyrosine both in vitro intact lymphocytes. Here we show α-tubulin present within the cytoskeletal microtubule network was phosphorylated Syk-dependent manner following activation of B-cells by engagement antigen receptor or treatment with phosphotyrosine phosphatase inhibitor, pervanadate. Immunofluorescence staining cytoskeletons western blotting studies...
Abstract Dyneins are responsible for essential movements in eukaryotic cells. The motor activity of each dynein complex resides its complement heavy chains. In the present study, we examined 136 chain sequences from completed genomes 11 diverse model organisms, including examples Viridiplantae, Excavata, Chromalveolata, and Metazoa. many cases, discovered chains previously not identified. For example, Tetrahymena expresses a total 25 DYH genes rather than identified 14. nonaxonemal DYH1...
A report on research that explicates three models of pedagogical practice underpin and characterize inquiry instruction in a course-based experience.
ABSTRACT Organisms that have cilia or flagella express over a dozen dynein heavy chain genes. Of these genes, most appear to encode axonemal dyneins, one encodes conventional cytoplasmic (MAP1C DHC1a), and one, here referred as DHC1b, an unclassified chain. Previous analysis of sea urchin DHC1b (Gibbons et al. (1994)Mol. Biol. Cell 5, 57-70) indicated this isoform is either with unusual protein sequence whose expression increases during ciliogenesis. In the present study, we examined in rat...