A5021833108- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Career Development and Diversity
- SARS-CoV-2 detection and testing
- Plant Virus Research Studies
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Ubiquitin and proteasome pathways
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- Interdisciplinary Research and Collaboration
- Experimental Learning in Engineering
- Evaluation of Teaching Practices
- Biosensors and Analytical Detection
- Reproductive Physiology in Livestock
- SARS-CoV-2 and COVID-19 Research
- Doctoral Education Challenges and Solutions
- Signaling Pathways in Disease
- Educational Assessment and Pedagogy
- Cardiomyopathy and Myosin Studies
Lehigh University
2015-2025
Bethlehem Area School District
2013
Brown University
1982-1985
Yale University
1982
ABSTRACT Engaging large numbers of undergraduates in authentic scientific discovery is desirable but difficult to achieve. We have developed a general model which faculty and teaching assistants from diverse academic institutions are trained teach research course for first-year undergraduate students focused on bacteriophage genomics. The situated within broader context aimed at understanding viral diversity, such that collaborators with established researchers the field. Howard Hughes...
Based upon the three experimentally derived models of E. coli 23S rRNA (1-3) and partial model for yeast 26S (4), which was deduced by homology to coli, we a secondary structure Xenopus laevis 28S rRNA. This is first complete presented eukaryotic Compensatory base changes support general validity our offer help resolve correct in regions where they are different from one another. Eukaryotic rDNA longer than prokaryotic virtue introns, expansion segments transcribed spacers, all discussed...
We have determined the complete nucleotide sequence of Xenopus laevie 28S rDNA (4110 bp). In order to locate evolutionarily conserved regions within rDNA, we compared homologous sequences from yeast, Physarum . and E. coli Numerous homology are dispersed throughout entire length all four organisms. These a higher A+T base composition than remainder rDNA. The has nine major areas inserted when 23S total these inserts in is 83Z G+C, generally responsible for high (66Z) G+C content as whole....
The bacteriophage population is vast, dynamic, old, and genetically diverse. genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, replete with genes unknown function. To further explore this broad group bacteriophages, we describe here isolation genomic characterization 116 Microbacterium spp. Most are lytic, can grouped into twelve clusters according their overall relatedness; seven singletons no close...
We have determined the sequence of rDNA region between 28Sα and 28Sβ rRRA coding segments (termed a “gap”) in insect Sciara corprophila used S1 nuclease mapping cDNA primer extension to define 5′ 3′ boundaries gap. Only 19 bases found at gap are absent from mature 28S rRNA. Eukaryotic rRNAs contain stretches nucleotides (“expansion segments”) which E.coli The is located within expansion segment V. Therefore, excision suggests that large portion V plays no function ribosomes. Specific...
The functional significance of ribosome heterogeneity in development and differentiation is relatively unexplored. We present the first vivo evidence playing a role specific mRNA translation multicellular eukaryote. Eukaryotic-specific ribosomal protein paralogues eRpL22 eRpL22-like are essential required for sperm maturation fertility Drosophila. roles spermatogenesis not completely interchangeable. Flies depleted rescued by overexpression have reduced fertility, confirming that cannot...
A report on research that explicates three models of pedagogical practice underpin and characterize inquiry instruction in a course-based experience.
Several ribosomal protein families contain paralogues whose roles may be equivalent or specialized to include extra-ribosomal functions. RpL22e family members rpL22 and rpL22-like are differentially expressed in Drosophila melanogaster: mRNA is gonad specific whereas ubiquitously, suggesting distinctive paralogue To determine if RpL22-like has a divergent role gonads, expression was analysed by qRT-PCR western blots, respectively, showing enrichment of 34 kDa (predicted) testis, but not...
We have examined the kinetics of process by which ribosomes are exported from nucleus to cytoplasm using Xenopus laevis oocytes microinjected into germinal vesicle with radiolabeled or ribosomal subunits X. laevis, Tetrahymena thermophila, Escherichia coli. Microinjected eukaryotic mature redistributed oocyte an apparent carrier-mediated transport that exhibits saturation as increasing amounts injected. T. thermophila competent traverse nuclear envelope, suggesting basic mechanism underlying...
A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host,
Mycobacteriophages – viruses of mycobacterial hosts are genetically diverse but morphologically all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group five closely related mycobacteriophages Corndog, Catdawg, Dylan, Firecracker, YungJamal designated as Cluster O long flexible tails unusual prolate capsids. Proteomic analysis phage Corndog particles, Catdawg Corndog-infected cells confirms expression half predicted gene products indicates non-canonical...
Duplicated ribosomal protein (Rp) gene families often encode highly similar or identical proteins with redundant unique roles. Eukaryotic-specific paralogues RpL22e and RpL22e-like-PA are structurally divergent within the N terminus differentially expressed, suggesting tissue-specific functions. We previously identified as a testis Rp. Strikingly, is detected in immunoblots at its expected molecular mass (m) of 33 kD increasing m ~43–55 kD, post-translational modification (PTM). Numerous...
Duplicated ribosomal protein (RP) genes in the Drosophila melanogaster eRpL22 family encode structurally-divergent and differentially-expressed rRNA-binding RPs. is expressed ubiquitously eRpL22-like expression tissue-restricted with highest levels adult male germline. We explored paralogue functional equivalence using GAL4-UAS system for knockdown or overexpression a conditional knockout heat- shock flippase/FRT line. Ubiquitous Actin-GAL4 resulted embryonic lethality, confirming...
Abstract In an attempt to understand more fully processes that control the selection or recruitment of follicles for ovulation, superovulation paradigm in combination with androgen, 5α‐dihydrotestosterone, antiandrogens, cyproterone acetate, was used immature mouse alter developmental potential destined ovulate become atretic. Quantitative analysis numbers eggs ovulated after one rounds stimulation by pregnant mare's serum gonadotrophin followed human chorionic (PMSG‐hCG), revealed a...
The professional identity of scientists has historically been cultivated to value research over teaching, which can undermine initiatives that aim reform science education. Course-Based Research Experiences (CRE) and the inclusive Education Communities (iREC) are two successful impactful efforts integrate teaching. this study is explicate instructors who implement a CRE within an established iREC explore how contributes success these programs. 97 from Science Alliance (SEA) participated in...
The proposed Drosophila melanogaster L23a ribosomal protein features a conserved C-terminal amino acid signature characteristic of other family members and unique N-terminal extension [Koyama et al. (Poly(ADP-ribose) polymerase interacts with novel proteins, L22 l23a, histone-like amino-terminal extensions. Gene 1999; 226: 339–345)], absent from Saccharomyces cerevisiae L25 that nearly doubles the size fly L23a. ability to replace role yeast in ribosome biogenesis was determined by creating...
ABSTRACT During lysogeny temperate phages establish a truce with the bacterial host. In this state, phage genome (prophage) is maintained within host environment. Consequently, many prophages have evolved systems to protect from heterotypic viral attack. This phenomenon of mediating defense their against competitor widespread among mycobacteriophages. We previously showed that Mycobacterium Butters prophage encodes two-component system (gp30/31) inhibits infection subset mycobacteriophages...
Differences in core or tissue-specific ribosomal protein (Rp) composition within ribosomes contribute to ribosome heterogeneity and functional variability. Yet, the degree which modulates development is unknown. The Drosophila melanogaster eRpL22 family contains structurally diverse paralogues, eRpL22-like. Unlike ubiquitously expressed eRpL22, eRpL22-like expression tissue-specific, notably male germline eye. We investigated developing eye uncover tissue/cell types where specific paralogue...
Species-specific pre-rRNA processing variations may result in fragmented 18S, 5.8S and 28S rRNAs. Some insect rRNAs are further cleaved, creating within a 'hidden break' or 'gap'. We investigated the specificity of mechanism by microinjecting Sciara coprophila (fungus fly) rDNA into Xenopus laevis oocytes to examine rRNA maturation cell where endogenous not cleaved at homologous sites. Results confirm transcription pre-28S fragmentation, demonstrating that fly-specific machinery is required....
The annotation of six cluster N Mycobacterium smegmatis phages (Kevin1, Nenae, Parmesanjohn, ShrimpFriedEgg, Smurph, and SpongeBob) reveals regions genomic diversity, particularly within the central region genome. genome Kevin1 includes two orphams (genes with no similarity to other phage genes), one predicted encode an AAA-ATPase.
Over the last two decades, there have been numerous initiatives to improve undergraduate student outcomes in STEM. One model for scalable reform is inclusive Research Education Community (iREC). In an iREC, STEM faculty from colleges and universities across nation are supported adopt sustainably implement course-based research – a form of science pedagogy that enhances learning persistence science. this study, we used pathway modeling develop qualitative description explicates HHMI Science...