A5004786664- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Glutathione Transferases and Polymorphisms
- Immune Cell Function and Interaction
- Lipid metabolism and disorders
- Glycosylation and Glycoproteins Research
- American Constitutional Law and Politics
- Seismic Waves and Analysis
- Oceanographic and Atmospheric Processes
- Diabetes and associated disorders
- Career Development and Diversity
- Higher Education Research Studies
- Genomics and Phylogenetic Studies
- Seismic Imaging and Inversion Techniques
- Pancreatitis Pathology and Treatment
- T-cell and B-cell Immunology
- Glioma Diagnosis and Treatment
- Transgenic Plants and Applications
Dana-Farber Cancer Institute
2021-2024
Mount St. Mary's University
2009-2024
Bridge University
2024
University of North Carolina at Chapel Hill
2024
Commonwealth Scientific and Industrial Research Organisation
2022
Harvard University
2021
United States Army Medical Research Institute of Infectious Diseases
2015
Loyola University Chicago
2003-2006
Recent work demonstrated that the Niemann-Pick C1 (NPC1) protein is an essential entry receptor for filoviruses. While previous studies focused on filovirus requirements of NPC1 in vitro, its roles replication and pathogenesis vivo remain unclear. Here, we evaluated importance NPC1, partner cholesterol transport, NPC2, by using a mouse model Ebolavirus (EBOV) disease. We found that, whereas wild-type mice had high viral loads succumbed to EBOV infection, Npc1(-/-) were entirely free...
Abstract Oncogenic mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 occur a wide range of cancers, including acute myeloid leukemia (AML) glioma. Mutant IDH enzymes convert 2-oxoglutarate (2OG) to (R)-2-hydroxyglutarate [(R)-2HG], an oncometabolite that is hypothesized promote cellular transformation by dysregulating 2OG-dependent enzymes. The only (R)-2HG target has been convincingly shown contribute mutant the tumor suppressor TET2. However, there ample evidence suggest other...
Kathryn Gunn1 and Julie-Aurore Losman1,2 1Division of Molecular Cellular Oncology, Department Medical Dana-Farber Cancer Institute, Boston, Massachusetts 02215, USA 2Division Hematology, Medicine, Brigham Women's Hospital, 02115, Correspondence: julieaurore_losman{at}dfci.harvard.edu
Summary acs encodes acetyl‐coenzyme A synthetase, a high‐affinity enzyme that allows cells to scavenge for acetate during carbon starvation. CRP activates transcription by binding tandem DNA sites located upstream of the major promoter, P2. Here, we used electrophoretic mobility shift assays and DNase I footprint analyses demonstrate nucleoid proteins FIS IHF each bind multiple within regulatory region, competes successfully with their overlapping neighbouring binds independently either or...
Abstract Marginal zone B (MZB) cells are the first splenic to initiate Ab secretion against polysaccharide-encapsulated Ags in vivo. This swift MZB cell response can be reproduced vitro as LPS treatment induces little 12 h. Conversely, of follicular (FOB) results after 2–3 days. The basis for these distinct kinetics is not understood. We performed ex vivo analysis resting and LPS-stimulated murine FOB found that express higher levels TLR complex RP105/MD-1 respond much lower concentrations...
CTP:phosphocholine cytidylyltransferase (CCT) is a key rate-controlling enzyme in the biosynthetic pathway leading to principle membrane phospholipid, phosphatidylcholine. CCTalpha predominant isoform expressed mammalian cells. To investigate role of development and function B-lymphocytes, mice with B-lymphocytes that selectively lacked were derived using CD19-driven Cre/loxP system. When challenged T-cell-dependent antigen, animals harboring CCTalpha-deficient B-cells exhibited hyper-IgM...
Lipoprotein lipase (LPL) is an enzyme that hydrolyzes large triglyceride-containing lipoprotein particles into free fatty acids can be taken up by adipose or muscle cells for energy storage use. LPL activity inhibited members of the angiopoietin-like (ANGPTL) protein family, which includes ANGPTL3 and ANGPTL4, both form a multimeric complex with ANGPTL8. While it known ANGPTL3/8 more potent inhibitor than alone, we lack macromolecular information on useful in understanding its interaction...
We describe a structured inquiry laboratory exercise that examines transcriptional regulation of the NOS2 gene under conditions simulate inflammatory response in macrophages. Using quantitative PCR and comparative CT method, students are able determine whether activation occurs to what degree. The is aimed at second year undergraduates who possess basic knowledge expression events. It requires only 4-5 hr dedicated time focuses on use primary literature, data analysis, interpretation....
This laboratory module simulates the process used by working scientists to ask and answer a question of biological interest. Instructors facilitate acquisition knowledge using comprehensive, inquiry-based approach in which students learn theory, hypothesis development, experimental design, data interpretation presentation. Using inflammation macrophages as model system, perform series molecular biology techniques address question: "Does stimulus 'X' induce inflammation?" To this question,...
<p>Characterization of isogenic TF-1 cell lines expressing TET2-targeting shRNAs.</p>
<p>Genomic regions marked by increased trimethyl-H3K4 are enriched in IDH1-mutant glioma MCTS lines when compared to IDH1-wild-type lines.</p>
<p>ChIP-seq analysis of primary AML patient samples.</p>
<p>Genomic regions marked by increased trimethyl-H3K4 are enriched in IDH1-mutant glioma MCTS lines when compared to IDH1-wild-type lines.</p>
<p>Characterization of KDM5A mutants.</p>
<p>Characterization of isogenic TF-1 cell lines expressing TET2-targeting shRNAs.</p>
<p>Trimethyl-H3K4 is not enriched upon knock-down of TET2 in TF-1 cells.</p>
<p>Transcriptional analysis of primary AML patient samples.</p>