A5016848835- Ubiquitin and proteasome pathways
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Toxoplasma gondii Research Studies
- Career Development and Diversity
- Nuclear Structure and Function
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- T-cell and Retrovirus Studies
- Cervical Cancer and HPV Research
- Mosquito-borne diseases and control
- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
- Entomopathogenic Microorganisms in Pest Control
- Complement system in diseases
- Doctoral Education Challenges and Solutions
- ATP Synthase and ATPases Research
- Malaria Research and Control
- Cellular Mechanics and Interactions
The University of Texas at El Paso
2009-2025
Texas A&M Health Science Center
2006-2012
Texas A&M University System
2004-2005
Texas A&M University
2003
Mitchell Institute
2003
Institute of Entomology
1999-2001
Arkansas Agricultural Experiment Station
2001
Post-translational modification by the conjugation of small ubiquitin-like modifiers is an essential mechanism to affect protein function. Currently, only a limited number substrates are known for most these modifiers, thus limiting our knowledge their role and relevance cellular physiology. Here, we report development universal strategy proteomic studies modifiers. This involves stable transfected cell lines expressing double-tagged modifier under control tightly negatively regulated...
The occlusion derived form of baculovirus is specially adapted for primary infection the host midgut epithelium. As such, virion must contain proteins essential range determination and initiation infection. Because knowledge composition a prerequisite functional investigation, this study used combination techniques to identify present within or associated with occlusion-derived virus (ODV) virion. Thirty-one proteins, including known be viral DNA replication, were identified confidence. An...
X-linked inhibitor of apoptosis protein (XIAP) overexpression has been found to be associated with malignant cancer progression and aggression in individuals many types cancers. However, the molecular basis XIAP regulation cell biological behavior remains largely unknown. In this study, we that a deficiency expression human cells by either knock-out or knockdown leads marked reduction β-actin polymerization cytoskeleton formation. Consistently, migration invasion were also decreased...
Abstract Metronomic, or continuous low-dose, chemotherapy can inhibit tumor growth via inhibition angiogenesis and immune system activation. At AACR 2024, we reported multiplex analysis of 75 cytokines in 34 out 38 patients with gastrointestinal (GI) tumors, who were evaluated a phase II clinical trial metronomic UFT (a 5-fluorouracil prodrug; 100 mg/twice per day p.o.) cyclophosphamide (an i.v. bolus on 1 500mg/mq2 then 50 mg/day plus celecoxib (200 p.o.); the COMET (CLINICAL ID...
ABSTRACT Previous reports indicate that mutations within the Autographa californica nucleopolyhedrosis virus FP25K gene (open reading frame 61) significantly reduce incorporation of enveloped nucleocapsids into viral occlusions. We report is a nucleocapsid protein both budded (BV) and occluded (ODV), we describe effects two (480-1 [N-terminal truncation] FP-βgal [C-terminal fusion]) on expression cellular localization ODV-E66 ODV-E25. Significantly decreased amounts are detected in cells...
SUMO modification regulates the activity of numerous transcription factors that have a direct role in cell-cycle progression, apoptosis, cellular proliferation, and development, but its differentiation processes is less clear. Keratinocyte requires coordinated activation series factors, as several crucial keratinocyte are known to be substrates, we investigated sumoylation differentiation. In human cell line model (HaCaT cells), Ca2+-induced led transient transcriptional genes encoding...
Our pioneering studies on the interplay between small ubiquitin-like modifier (SUMO) and influenza A virus identified nonstructural protein NS1 as first known SUMO target of one most abundantly SUMOylated proteins. Here, we further characterize role SUMOylation for A/Puerto Rico/8/1934 (PR8) protein, demonstrating that is not only by SUMO1 but also SUMO2/3 mapping main sites in to residues K219 K70. Furthermore, using SUMOylatable non-SUMOylatable forms an NS1-specific artificial ligase...
A report on research that explicates three models of pedagogical practice underpin and characterize inquiry instruction in a course-based experience.
orf101 is a late gene of Autographa californica nucleopolyhedrovirus (AcMNPV). It encodes protein 42 kDa which component the nucleocapsid budded virus (BV) and occlusion-derived (ODV). To reflect this viral localization, product was named BV/ODV-C42 (C42). C42 predominantly detected within infected-cell nucleus: at 24 h postinfection (p.i.), it coincident with virogenic stroma, but by 72 p.i., stroma minimally labeled while more uniformly located throughout nucleus. Yeast two-hybrid screens...
Abstract Substantial increases in the conjugation of main human SUMO paralogs, SUMO1, SUMO2, and SUMO3, are observed upon exposure to different cellular stressors, such considered important facilitate cell survival stress. Despite their critical role, little is known about how levels modifiers regulated cell, particularly as it relates changes Here we characterize contribution alternative splicing towards regulating expression paralogs under normalcy three stress conditions, heat-shock,...
ABSTRACT Partial deletions within Autographa californica open reading frame 61 ( FP25K ) alter the expression and accumulation profile of several viral proteins transport occlusion-derived virus (ODV)-E66 to intranuclear membranes during infection (S. C. Braunagel et al., J. Virol. 73:8559–8570, 1999). Here we show effects a full deletion overexpression on two ODV envelope proteins, ODV-E66 (E66) ODV-E25 (E25). Deletion substantially altered levels E66 infection. Compared with cells infected...
ABSTRACT Papillomavirus DNA replication occurs in the nucleus of infected cells and requires viral E1 protein, which enters nuclei host epithelial carries out enzymatic functions required for initiation replication. In this study, we investigated pathway regulation nuclear import protein from bovine papillomavirus type 1 (BPV1). Using an vitro binding assay, determined that interacted with importins α3, α4, α5 via its localization signal (NLS) sequence. agreement result, purified was...
The professional identity of scientists has historically been cultivated to value research over teaching, which can undermine initiatives that aim reform science education. Course-Based Research Experiences (CRE) and the inclusive Education Communities (iREC) are two successful impactful efforts integrate teaching. this study is explicate instructors who implement a CRE within an established iREC explore how contributes success these programs. 97 from Science Alliance (SEA) participated in...
Sumoylation is a highly dynamic process that plays role in multitude of processes ranging from cell cycle progression to mRNA processing and cancer. A previous study our lab demonstrated SUMO an important keratinocyte differentiation. Here we present new method tracking the sumoylation state proteins by creating stably transfected HaCaT line expressing inducible SNAP-SUMO3 protein. The SNAP-tag allows covalent fluorescent labeling denaturation resistant. When combined with two-dimensional...