A5077439703- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Chromatin Remodeling and Cancer
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cell Adhesion Molecules Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- vaccines and immunoinformatics approaches
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Renal and related cancers
University of Oklahoma Health Sciences Center
2010-2023
Oklahoma City University
2018
University of Oklahoma
2016-2018
Institute for New Economic Thinking
2018
Mayo Clinic Health System
2017
OU Health
1998-2016
Oklahoma Medical Research Foundation
2002-2015
Bipar
2008
The University of Texas Southwestern Medical Center
1989-1999
The University of Texas at Austin
1999
Abstract Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded principles open access and collaboration. Our consortium over 100 researchers has collectively contributed 130 pertinent human, mouse rat TFs. Notable features TFe website include high-quality PDF generator web API for programmatic data retrieval. aims to rapidly educate scientists about TFs they encounter through delivery...
Bright/Arid3a has been characterized both as an activator of immunoglobulin heavy-chain transcription and a proto-oncogene. Although Bright expression is highly B lineage stage restricted in adult mice, its the earliest identifiable hematopoietic stem cell (HSC) population suggests that might have additional functions. We showed >99% Bright−/− embryos die at midgestation from failed hematopoiesis. embryonic day 12.5 (E12.5) fetal livers increase immature markers. Colony-forming assays...
Matrix-associated regions (MARs), AT-rich DNA segments that have an affinity for the nuclear matrix, been shown to play a role in transcriptional regulation of eukaryotic genes. The present study demonstrates element, called L2a, which has implicated mouse<i>CD8a</i> gene encoding important T cell coreceptor, is MAR. Moreover, identities two proteins, L2a-P1 and L2a-P2, previously bind L2a determined. protein found be all CD8-positive lines tested SATB1, known MAR-binding protein. widely...
We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming mouse embryonic fibroblasts (MEFs) and enhancement standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under stem cell (ESC) culture conditions, spontaneously form clones in vitro express pluripotency markers, differentiate all germ lineages, vivo teratomas chimeric mice. demonstrate BRIGHT binds directly promoter/enhancer regions Oct4, Sox2, Nanog contribute...
Bright/ARID3a/Dril1, a member of the ARID family transcription factors, is expressed in highly regulated fashion B lymphocytes, where it enhances immunoglobulin three- to sixfold. Recent publications from our lab indicated that functional, but not kinase-inactive, Bruton's tyrosine kinase (Btk) critical for Bright activity an vitro model system, yet itself appreciably phosphorylated. These data suggested third protein, and Btk substrate, must contribute Bright-enhanced transcription. The...
Abstract Retinoids are known to have potent effects on hemopoietic stem cell integrity, and our objective was learn whether they influence cells destined replenish the immune system. Total CD19+ B lineage increased substantially in marrow spleens of all-trans retinoic acid (ATRA)-treated C57BL6 mice, while lymphoid progenitors were reduced. All targets ATRA culture overall yields declined without reductions proliferation. Remarkably, shortened time required for primitive generate cells. PCR...
B-cell regulator of immunoglobulin heavy chain transcription (Bright)/ARID3a, an A+T-rich interaction domain protein, was originally discovered in B lymphocyte lineage cells. However, expression patterns and high lethality levels knockout mice suggested that it had additional functions. Three independent lines evidence show functional inhibition Bright results increased developmental plasticity. Bright-deficient cells from two mouse models expressed a number pluripotency-associated gene...
Abstract Binding of the transcription factor Bright to Ig heavy chain loci after B cell activation is associated with increased transcription. We now report that coprecipitates Bruton’s tyrosine kinase (Btk), defective enzyme in X-linked immunodeficiency disease (xid). Furthermore, we observed Btk nucleus activated murine cells, and mobility shift assays suggest it a component DNA-binding complex. While protein was synthesized spleen cells from xid mice, did not bind DNA or associate stably...
In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 -200 bp upstream of mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Because high A + T/G C ratio these sequences consistent findings others that enhancer- promoterlike regions are often located near matrix-associated regions, asked whether might also be...
The B cell regulator of Ig heavy chain transcription (Bright) is a DNA-binding protein that was originally discovered in mature Ag-specific line after stimulation with IL-5 and Ag. It binds to the intronic enhancer 5' V1 S107 family V(H) promoter. Several studies suggested Bright may increase locus, expression lines limited those representing cells. We have now analyzed normal hemopoietic tissues for during lymphocyte differentiation. expected find subset spleen cells, but also observed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExpression and sequence analyses of serum amyloid A in the Syrian hamsterC. F. Webb, P. W. Tucker, S. B. DowtonCite this: Biochemistry 1989, 28, 11, 4785–4790Publication Date (Print):May 30, 1989Publication History Published online1 May 2002Published inissue 30 1989https://doi.org/10.1021/bi00437a040Request reuse permissionsArticle Views46Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article...
Although much has been learned about basal levels of immunoglobulin (Ig) transcription, the regulatory effects cytokines and antigen (Ag) upon Ig expression in lymphocytes have not fully characterized. We previously reported that Ag plus interleukin-5 (IL-5) caused increased steady-state mRNA Ag-specific cell lines. In this study, we identified a region between -250 -125 bp 5' transcription start site is necessary for induction mu by IL-5. Mobility shift UV cross-linking studies indicated...
The radiosensitization of exponentially-growing V79-171 cells whose DNA has been substituted by bromodeoxyuridine (BrdU) in place thymidine is decreased if acetone present during irradiation. Acetone, at a concentration 1 mol dm-3, removes the majority increase double-strand breaks (dsbs) caused BrdU substitution, but only approximately half cell killing. decrease coincides with removal additional dsbs. protection afforded against dsbs assumed to be due its ability scavenge hydrated...
We have established an Ag-specific in vitro system for studying the roles of Ag and IL-5 B cell differentiation Ig production. The murine leukemia, BCL1B1, was transfected with mu kappa genomic sequences VS107 V regions that conferred a T15 Id phosphocholine-binding specificity upon cells. Transfected cells were treated both T-dependent phosphorylcholine-key-hole limpet hemocyanin (PC-KLH), 0.5 ng/ml purified IL-5. After 3.5 days steady state mu-mRNA levels increased three- to fourfold over...
Abstract We recently reported that the transcription factor ARID3a is expressed in a subset of human hematopoietic progenitor stem cells both healthy individuals and patients with systemic lupus erythematosus. Numbers ARID3a+ were associated increased production autoreactive Abs when those introduced into humanized mouse models. Although ARID3a/Bright knockout mice died utero, they exhibited decreased numbers erythrocytes, indicating functionally important for hematopoiesis mice. To explore...
Objective Systemic lupus erythematosus (SLE) is a complex and multifactorial autoimmune disease with striking clinical, immunologic, genetic heterogeneity, despite nearly ubiquitous antinuclear antibody (ANA) production. Multiple gene polymorphisms have been associated the disease, but these individually account for only very small percentage of overall SLE risk. In earlier studies, constitutive expression DNA‐binding protein AT‐rich–interactive domain 3A (ARID3a) in transgenic mouse B...
Abstract A major component in controlling V(D)J recombination is differential accessibility through localized changes chromatin structure. Attachment of DNA to the nuclear matrix via attachment region (MAR) sequences, and interaction with MAR-binding proteins have been shown alter conformation, promote histone acetylation, influence gene transcription. In this study, flanking regions several human mouse Ig VH Vκ genes were analyzed extensively for presence MARs by vitro matrix-binding assay,...
Bright (B-cell regulator of immunoglobulin heavy chain transcription) binding to loci after B-cell activation is associated with increased transcription. Our earlier reports demonstrated that coimmunoprecipitates Bruton's tyrosine kinase (Btk) and these proteins associate in a DNA-binding complex primary B cells. cells from immunodeficient mice mutation Btk failed produce stable complexes. In order determine if important for function, transcription assay was established analyzed using...