A5008953835- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Career Development and Diversity
- Fungal and yeast genetics research
- Biomedical and Engineering Education
- Genetics, Bioinformatics, and Biomedical Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- DNA Repair Mechanisms
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Interdisciplinary Research and Collaboration
- Service-Learning and Community Engagement
- Cancer-related gene regulation
- Advanced Fluorescence Microscopy Techniques
- Educational Strategies and Epistemologies
- Parasitic Diseases Research and Treatment
- Immunotherapy and Immune Responses
- Race, History, and American Society
- Retinoids in leukemia and cellular processes
- Multiple Sclerosis Research Studies
- Plant-Microbe Interactions and Immunity
- Fractal and DNA sequence analysis
- Technology Assessment and Management
- Diabetes Management and Research
Webster University
2010-2022
University of Southern California
2019
Fitzsimons Army Medical Center
2005
University of Colorado Anschutz Medical Campus
2004
University of Colorado Denver
2002
Vanderbilt University
1991-2001
Memorial Sloan Kettering Cancer Center
2000
Cornell University
2000
The University of Texas MD Anderson Cancer Center
1994
Rockefeller University
1990
The C-terminal heptad repeat domain (CTD) of RNA polymerase II (pol II) is proposed to target pre-mRNA processing enzymes nascent pol transcripts, but this idea has not been directly tested in vivo. In vitro, the yeast mRNA capping Ceg1 and Abd1 bind specifically phosphorylated CTD. Here we show that cross-link vivo 5′ ends transcribed genes localization requires Both extent CTD phosphorylation at Ser 5 binding decreased as moved from 3′ ACT1, ENO2, TEF1, GAL1 , GAL10 genes. released early...
Genomics is not only essential for students to understand biology but also provides unprecedented opportunities undergraduate research. The goal of the Education Partnership (GEP), a collaboration between growing number colleges and universities around country Department Biology Genome Center Washington University in St. Louis, provide such research opportunities. Using versatile curriculum that has been adapted many different class settings, GEP undergraduates undertake projects bring...
There is widespread agreement that science, technology, engineering, and mathematics programs should provide undergraduates with research experience. Practical issues limited resources, however, make this a challenge. We have developed bioinformatics project provides course-based experience for students at diverse group of schools offers the opportunity to tailor local curriculum institution-specific student needs. assessed both attitude knowledge gains, looking insights into how respond...
The gene encoding the Schizosaccharomyces pombe TATA box-binding factor (TFIID) was cloned and sequenced. contains three introns codes for a polypeptide of 231 amino acids. cDNA-expressed protein showed both basal transcription activities. carboxy-terminal three-quarters S. TFIID shares an extraordinary degree acid sequence homology with corresponding region Saccharomyces cerevisiae that has been shown to be necessary sufficient In contrast, amino-terminal regions TFIIDs differ markedly in...
In their 2012 report, the President's Council of Advisors on Science and Technology advocated “replacing standard science laboratory courses with discovery-based research courses”—a challenging proposition that presents practical pedagogical difficulties. this paper, we describe our collective experiences working Genomics Education Partnership, a nationwide faculty consortium aims to provide undergraduates experience in genomics through scheduled course (a classroom-based undergraduate...
A hallmark of the research experience is encountering difficulty and working through those challenges to achieve success. This ability essential being a successful scientist, but replicating such in teaching setting can be difficult. The Genomics Education Partnership (GEP) consortium faculty who engage their students genomics Course-Based Undergraduate Research Experience (CURE). Students participate genome annotation, generating gene models using multiple lines experimental evidence. Our...
Members of the MyoD family helix-loop-helix proteins control expression muscle phenotype by regulating activity subordinate genes. To investigate processes that myogenic factors and regulate establishment maintenance skeletal phenotype, we have analyzed sequences necessary for transcription maternally expressed Xenopus (XMyoD) gene. A 3.5-kb DNA fragment containing XMyoDa promoter was in a somite-specific manner injected frog embryos. The active oocytes cultured cells but not fibroblasts or...
A report on research that explicates three models of pedagogical practice underpin and characterize inquiry instruction in a course-based experience.
We have examined the structure-function relationships of TFIID through in vivo complementation tests. A yeast strain was constructed which lacked chromosomal copy SPT15, gene encoding TFIID, and therefore dependent on a functional plasmid-borne wild-type this for viability. By using plasmid shuffle technique, replaced with family plasmids containing series systematically mutated genes. These various forms were expressed from three different promoter contexts strengths, ability each mutant...
The M26 meiotic recombination hot spot in the ade6 gene of Schizosaccharomyces pombe is activated by heterodimeric binding protein Mts1-Mts2. individual Mts1 (Atf1, Gad7) and Mts2 (Pcr1) proteins are also transcription factors involved developmental decisions. We report that Mts key effectors at least two distinct classes decisions regulated mitogen-activated (MAP) kinase cascade. first class (osmoregulation, spore viability, quiescence) requires Spc1 MAP but does not require protein. second...
The Genomics Education Partnership (GEP) engages students in a course-based undergraduate research experience (CURE). To better understand the student attributes that support success this CURE, we asked about their attitudes using previously published scales measure epistemic beliefs work and science, interest grit. We found, general, bring with them into classroom contribute to two outcome measures, namely, learning as assessed by pre- postquiz perceived self-reported benefits. While GEP...
We have used a combination of fluorescence anisotropy spectroscopy and fluorescence-based native gel electrophoresis methods to examine the effects transcription factor IID-specific subunit TAF130p (TAF145p) upon TATA box DNA binding properties box-binding protein (TBP). Purified full-length recombinant decreases TBP-TATA complex formation at equilibrium by competing directly with for TBP. Interestingly, we found that is capable multiple molecules TBP nanomolar affinity. The biological...
We describe the quantitative monitoring of TATA-binding protein (TBP) localization and expression in living Saccharomyces cerevisiae cells. replaced endogenous TBP with a green fluorescent (GFP) · fusion, which was imaged quantitatively by laser scanning confocal microscopy (LSCM). When GFP altered using various promoters, levels measured LSCM correlated well determined immunoblot whole cell extract protein. These results show that imaging not only offers method measurement equivalent to...
We have examined the structure-function relationships of TFIID through in vivo complementation tests. A yeast strain was constructed which lacked chromosomal copy SPT15, gene encoding TFIID, and therefore dependent on a functional plasmid-borne wild-type this for viability. By using plasmid shuffle technique, replaced with family plasmids containing series systematically mutated genes. These various forms were expressed from three different promoter contexts strengths, ability each mutant...
In this report we describe studies which utilized yeast strains bearing gain and loss of function alleles ofABF1 in order to attempt directly implicate Abf1p modulating transcription the TBP-encoding gene, SPT15, vivo. We found that overexpression a cell increased gene stimulation depended upon exact sequence binding site (ABF1) present gene. Further, strain expressing temperature sensitive form Abf1p, occupancy chromosomal ABF1 was immediately lost following shift. Both results suggest...
TBP, the TATA-box binding protein, plays a key role in eukaryotic gene transcription since it is required for initiation by all three nuclear DNA-dependent RNA polymerases. In order to gain insight into mechanisms of regulation this basal factor, we undertook mutational analysis sequences involved directing encoding TBP Saccharomyces cerevisiae. An extensive family mutations promoter were fused Escherichia coli reporter lacZ, transferred back yeast, and assayed their ability direct...
Members of the MyoD family helix-loop-helix proteins control expression muscle phenotype by regulating activity subordinate genes. To investigate processes that myogenic factors and regulate establishment maintenance skeletal phenotype, we have analyzed sequences necessary for transcription maternally expressed Xenopus (XMyoD) gene. A 3.5-kb DNA fragment containing XMyoDa promoter was in a somite-specific manner injected frog embryos. The active oocytes cultured cells but not fibroblasts or...
We previously demonstrated that a combination of both positive and negative cis-acting upstream elements control the transcription gene encoding TBP (SPT15) in Saccharomyces cerevisiae. One these found study, resident between 5′ flanking sequences −147 −128 , termed PED (for element distal), was to play an essential role driving TBP. In this report, we map at nucleotide-level resolution, critical residues which comprise PED, purify sequence protein binds it determine binding factor is Abf1p,...
Abstract As we train the next generation of Science, Technology, Engineering, and Math (STEM) researchers, it is imperative that expand our recruitment to community college students. Many these students are highly motivated extremely talented, but they often lack exposure cutting-edge technology found at R1 institutions, much less have opportunities participate in original research. The Center for Dark Energy Biosphere Investigations (C-DEBI) University Southern California (USC) started a...
The effect of Anderson localization in DNA on the Auger destruction by Coulombic explosion at ionized radiation has been theoretically discussed present work. theory modified taking into account localized and delocalized electron states owing to correlated disorder a sequence nucleotides. According theoretical model destruction, dominant ratio exon compared intron results stronger resistance exons irradiation causing Auger-cascade process than introns.