A5029918930- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Invertebrate Immune Response Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Axon Guidance and Neuronal Signaling
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Genomic variations and chromosomal abnormalities
- Gene Regulatory Network Analysis
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- Career Development and Diversity
- Heat shock proteins research
- Cholinesterase and Neurodegenerative Diseases
- Insect Utilization and Effects
Duke University
2003-2023
Harvard University
1999-2005
Howard Hughes Medical Institute
1995-2005
Syngenta (United States)
2002
University of Illinois Urbana-Champaign
1993-1998
Columbia University
1994
ABSTRACT We report the identification of RK2, a glial-specific home-odomain protein. RK2 is localized to nucleus virtually all embryonic and imaginal glial cells, with exception midline glia. Embryos mutant for gene encoding are lethal but normal early gliogenesis (birth, initial divisions migration glia) axonogenesis (neuronal pathfinding fasciculation). However, later in development, there significantly fewer longitudinal glia that spatially disorganized; addition, slight disorganization...
ABSTRACT The central nervous system (CNS) represents an excellent model for examining how a multitude of unique cell fates are specified. We find that asymmetric localization the numb protein autonomously controls binary fate decision in Drosophila CNS. simplest lineage CNS is MP2 precursor: it divides unequally to generate dMP2 and vMP2 neurons. Both interneurons but project different directions: projects its axon posteriorly while anteriorly. During mitosis, localized into excluded from...
Wingless (Wg) is a secreted ligand that differentially activates gene expression in target tissues. It belongs to the Wnt family of signaling molecules regulate cell-to-cell interactions during development. Activation Wg targets dependent on concentration extracellular milieu; cellular mechanisms govern synthesis, delivery and receipt are elaborate complex. We have identified sprinter (srt), which encodes novel, evolutionarily conserved transmembrane protein required for transmission signal....
Genomics is not only essential for students to understand biology but also provides unprecedented opportunities undergraduate research. The goal of the Education Partnership (GEP), a collaboration between growing number colleges and universities around country Department Biology Genome Center Washington University in St. Louis, provide such research opportunities. Using versatile curriculum that has been adapted many different class settings, GEP undergraduates undertake projects bring...
There is widespread agreement that science, technology, engineering, and mathematics programs should provide undergraduates with research experience. Practical issues limited resources, however, make this a challenge. We have developed bioinformatics project provides course-based experience for students at diverse group of schools offers the opportunity to tailor local curriculum institution-specific student needs. assessed both attitude knowledge gains, looking insights into how respond...
Extensive departures from balanced gene dose in aneuploids are highly deleterious. However, we know very little about the relationship between copy number and expression aneuploid cells. We determined transcript abundance (expression) genome-wide Drosophila S2 cells by DNA-Seq RNA-Seq. found that for >43 Mb of genome, primarily range one to five copies, show a male genotype (∼ two X chromosomes four sets autosomes, or 2X;4A). Both autosomes showed dosage compensation. chromosome was elevated...
Notch signalling, which is highly conserved from nematodes to mammals, plays crucial roles in many developmental processes. In the Drosophila embryo, deficiency signalling results neural hyperplasia, commonly referred as neurogenic phenotype. We identify a novel maternal gene, neurotic, and show that it essential for signalling. neurotic encodes homolog of mammalian GDP-fucose protein O-fucosyltransferase, adds fucose sugar epidermal growth factor-like repeats known play role functions...
SUMMARY Dh, the gene that encodes a CRF-like peptide in Drosophila melanogaster, is described. The product of this 44-amino-acid (Drome-DH44) with sequence almost identical to Musca domestica and Stomoxys calcitrans diuretic hormones. There are no other similar peptides encoded within known genomic sequence. Functional studies showed deduced stimulated fluid production, effect was mediated by cyclic AMP principal cells only: there on levels either GMP or intracellular calcium. Stimulation...
In their 2012 report, the President's Council of Advisors on Science and Technology advocated “replacing standard science laboratory courses with discovery-based research courses”—a challenging proposition that presents practical pedagogical difficulties. this paper, we describe our collective experiences working Genomics Education Partnership, a nationwide faculty consortium aims to provide undergraduates experience in genomics through scheduled course (a classroom-based undergraduate...
Metabolism of inositol 1,4,5-trisphosphate (I(1,4,5)P3) results in the production diverse arrays polyphosphates (IPs), such as IP4, IP5, IP6) and PP-IP5. Insights into their synthesis metazoans are reported here through molecular studies fruit fly, Drosophila melanogaster. Two I(1,4,5)P3 kinase gene products implicated initiating catabolism these important IP regulators. We find dmIpk2 is a nucleocytoplasmic 6-/3-kinase that converts to I(1,3,4,5,6)P5, harbors 5-kinase activity toward...
The fruitless (fru) gene in Drosophila melanogaster is a multifunctional that has sex-specific functions the regulation of male sexual behavior and sex-nonspecific affecting adult viability external morphology. While much attention focused on fru's roles, less known about its functions. We have examined role embryonic neural development. fru transcripts from promoters are expressed beginning at earliest stages neurogenesis, Fru proteins present both neurons glia. In embryos lack most or all...
Abstract Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in vital gene cannot survive unless there is wild-type copy duplicated elsewhere genome. Rescuing lethality an with duplication allows to be used experimentally complementation tests and other genetic crosses it maps mutated defined chromosomal region. Duplications can also screen dosage-dependent enhancers suppressors mutant phenotypes as way identify genes involved...
Abstract The discordance between genome size and the complexity of eukaryotes can partly be attributed to differences in repeat density. Muller F element (∼5.2 Mb) is smallest chromosome Drosophila melanogaster, but it substantially larger (>18.7 D. ananassae. To identify major contributors expansion assess their impact, we improved sequence annotated genes a 1.4-Mb region ananassae element, 1.7-Mb from D for comparison. We find that transposons (particularly LTR LINE...
Significance Inositol phosphate kinase 2 (Ipk2) is a conserved protein that initiates the production of inositol intracellular messengers are critical for regulating variety cellular processes. Here we explore developmental roles Ipk2 and its products in Drosophila . We report activity required to develop adult body structures including eyes, legs, wings, which formed by tissue known as imaginal discs. Although mutant discs seem pattern normally embryogenesis, during larval development they...
The pigmentation mutation