A5030282445- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Sphingolipid Metabolism and Signaling
- Congenital heart defects research
- Silk-based biomaterials and applications
- Chromosomal and Genetic Variations
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cellular Mechanics and Interactions
- Lysosomal Storage Disorders Research
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- Aquaculture disease management and microbiota
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Insect and Arachnid Ecology and Behavior
- RNA Research and Splicing
- RNA regulation and disease
- melanin and skin pigmentation
- Cancer-related Molecular Pathways
Washington University in St. Louis
2013-2024
University of Missouri–Kansas City
2024
University of Illinois Urbana-Champaign
1995-1998
Howard Hughes Medical Institute
1991-1998
Harvard University
1996
Brigham and Women's Hospital
1996
University of Wisconsin–Madison
1991-1994
Haverford College
1990-1991
Adult Drosophila possess a large number of sensory organs, including and small bristles other types sensilla, each arising from single mother cell at particular positions in reproducible pattern. Genetic studies have shown that organ pattern formation is partly coordinated by structurally similar, potential heterodimer-forming, helix-loop-helix (HLH) regulatory proteins. Here, localizing gene expression during the development normal mutant imaginal discs, we show two positive regulators...
After invagination of the mesodermal primordium in gastrulating Drosophila embryo, internalized cells migrate a dorsolateral direction along overlying ectoderm. This movement generates stereotyped arrangement that is essential for their correct patterning by later position-specific inductive signals. We now report proper cell migration dependent on function fibroblast growth factor (FGF) receptor encoded heartless (htl). In htl mutant embryos, mesoderm forms normally but fails to undergo its...
Abstract The Drosophila embryonic central nervous system develops from sets of progenitor neuroblasts which segregate the neuroectoderm during early embryogenesis. Cells within this region can follow either neural or epidermal developmental pathway, a decision guided by two opposing classes genes. proneural genes, including members achaete-scute complex (AS-C), promote neurogenesis, while neurogenic genes prevent neurogenesis and facilitate development. To understand role that gene...
ABSTRACT In Drosophila, most neuronal siblings have different fates (‘A/B’). Here we demonstrate that mutations in sanpodo, a tropomodulin actin-binding protein homologue, equalize diverse array of sibling neuron (‘B/B’). Loss Notch signaling gives the same phenotype, whereas loss numb opposite phenotype (‘A/A’). The identical effect removing either sanpodo or function on CNS neurons indicates may act pathway. addition, and show dosage-sensitive interactions epistasis experiments indicate...
ABSTRACT The Drosophila heart is a simple organ composed of two major cell types: cardioblasts, which form the contractile tube heart, and pericardial cells, flank cardioblasts. A complete understanding development requires identification all types that comprise elucidation cellular genetic mechanisms regulate these cells. Here, we report new population cells: Odd skipped-positive cells (Odd-pericardial cells). We have used descriptive, lineage tracing assays to clarify control...
ABSTRACT Drosophila possesses two FGF receptors which are encoded by the heartless and breathless genes. HEARTLESS is essential for early migration patterning of embryonic mesoderm, while BREATHLESS required proper branching tracheal system. We have identified a new gene, heartbroken, that participates in signalling pathways both receptors. Mutations heartbroken associated with defects later specification mesodermal cells. Genetic interaction epistasis experiments indicate acts downstream...
J B Skeath, G Panganiban, Selegue, and S Carroll Howard Hughes Medical Institute, University of Wisconsin-Madison 53706.
The MAPKKK dual leucine zipper-containing kinase (DLK, Wallenda in Drosophila ) is an evolutionarily conserved component of the axonal injury response pathway. After nerve injury, DLK promotes degeneration distal axons and regeneration proximal axons. This role coordinating suggests that may be a sensor axon so understanding how activated important. Two mechanisms are known to activate DLK. First, increasing levels via overexpression or loss PHR ubiquitin ligases target signaling. Second,...
A hallmark of mature skeletal muscles is the presence multinucleate muscle fibers. In Drosophila, formation syncytia requires cooperative participation two types myoblasts, founder cells and fusion-competent myoblasts. We show that a newly identified gene, lame duck (lmd), has an essential regulatory role in specification function Embryos lack lmd loss expression key differentiation fusion genes, Mef2 sticks-and-stones, myoblasts are completely devoid By contrast, specified retain their...
ABSTRACT The segmented portion of the Drosophila embryonic central nervous system develops from a bilaterally symmetrical, segmentally reiterated array 30 unique neural stem cells, called neuroblasts. first 15 neuroblasts form about 30-60 minutes after gastrulation in two sequential waves neuroblast segregation and are arranged three dorsoventral columns four anteroposterior rows per hemisegment. Each acquires identity, based on gene expression nearly invariant cell lineage it produces....
The Drosophila heart consists of two major cell types: cardioblasts, which form the contractile tube heart; and pericardial cells, flank cardioblasts are thought to filter detoxify blood or hemolymph fly. We present completion entire lineage all cells. Notably, we detect a previously unappreciated distinction between lineages cells located in posterior seven segments relative those more anteriorly. Using genetic screen, have identified ETS-transcription factor pointed as key regulator...
Endocytosis and trafficking within the endocytosis pathway are known to modulate activity of different signaling pathways. Epsins promote postulated target specific proteins for regulated endocytosis. Here, we present a functional link between Notchpathway epsins. We identify Drosophila ortholog epsin, liquid facets (lqf), as an inhibitor cardioblast development in genetic screen mutants that affect heart development. find lqf inhibits promotes fusion-competent myoblasts, suggesting model...
The extracellular matrix (ECM) regulates cell migration and sculpts organ shape. AdamTS proteins are metalloproteases known to modify ECM promote migration, but demonstrated roles for in regulating CNS structure ensuring lineages remain fixed place have not been uncovered. Using forward genetic approaches Drosophila, we find that reduction of AdamTS-A function induces both the mass exodus neural out drastic perturbations structure. Expressed active surface glia, acts parallel perlecan...