Srebrenka Robic
A5065436937- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Gene expression and cancer classification
- Lipid Membrane Structure and Behavior
- Chromosomal and Genetic Variations
- Career Development and Diversity
- Interdisciplinary Research and Collaboration
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Escherichia coli research studies
- Research Data Management Practices
- Various Chemistry Research Topics
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Plant Virus Research Studies
- Gut microbiota and health
- Grit, Self-Efficacy, and Motivation
- Biotin and Related Studies
- DNA Repair Mechanisms
- Spectroscopy and Quantum Chemical Studies
Agnes Scott College
2010-2022
Universidad de Granada
2003
University of California, Berkeley
2002-2003
University of Oregon
1997
There is widespread agreement that science, technology, engineering, and mathematics programs should provide undergraduates with research experience. Practical issues limited resources, however, make this a challenge. We have developed bioinformatics project provides course-based experience for students at diverse group of schools offers the opportunity to tailor local curriculum institution-specific student needs. assessed both attitude knowledge gains, looking insights into how respond...
Although bioinformatics is becoming increasingly central to research in the life sciences, skills and knowledge are not well integrated into undergraduate biology education. This curricular gap prevents students from harnessing full potential of their education, limiting career opportunities slowing innovation. To advance integration sciences a framework core competencies needed. that end, we here report results survey faculty United States about teaching scientists. Responses were received...
Abstract The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution repeats and genes, we manually improved sequence annotated genes on D. erecta, mojavensis, grimshawi elements euchromatic domains from D element. We find that have greater transposon density (25–50%) than reference regions (3–11%). Among elements, has lowest...
In their 2012 report, the President's Council of Advisors on Science and Technology advocated “replacing standard science laboratory courses with discovery-based research courses”—a challenging proposition that presents practical pedagogical difficulties. this paper, we describe our collective experiences working Genomics Education Partnership, a nationwide faculty consortium aims to provide undergraduates experience in genomics through scheduled course (a classroom-based undergraduate...
Bioinformatics, a discipline that combines aspects of biology, statistics, mathematics, and computer science, is becoming increasingly important for biological research. However, bioinformatics instruction not yet generally integrated into undergraduate life sciences curricula. To understand why we studied how being included in biology education the US by conducting nationwide survey faculty at two- four-year institutions. The asked several open-ended questions probed barriers to...
Ribonucleases H from the thermophilic bacterium Thermus thermophilus and mesophile Escherichia coli demonstrate a dramatic surprising difference in their change heat capacity upon unfolding (ΔCp°). The lower ΔCp° of protein directly contributes to its higher thermal denaturation temperature ( T m ). We propose that this originates residual structure unfolded state protein; we verify hypothesis by using mutagenic approach. Residual may provide mechanism for balancing high with optimal...
A hallmark of the research experience is encountering difficulty and working through those challenges to achieve success. This ability essential being a successful scientist, but replicating such in teaching setting can be difficult. The Genomics Education Partnership (GEP) consortium faculty who engage their students genomics Course-Based Undergraduate Research Experience (CURE). Students participate genome annotation, generating gene models using multiple lines experimental evidence. Our...
To fully understand the roles proteins play in cellular processes, students need to grasp complex ideas about protein structure, folding, and stability. Our current understanding of these topics is based on mathematical models experimental data. However, stability are often introduced as descriptive, qualitative phenomena undergraduate classes. In process learning topics, form incorrect ideas. For example, by folding context synthesis, may come an conclusion that once synthesized ribosome, a...
Abstract The discordance between genome size and the complexity of eukaryotes can partly be attributed to differences in repeat density. Muller F element (∼5.2 Mb) is smallest chromosome Drosophila melanogaster, but it substantially larger (>18.7 D. ananassae. To identify major contributors expansion assess their impact, we improved sequence annotated genes a 1.4-Mb region ananassae element, 1.7-Mb from D for comparison. We find that transposons (particularly LTR LINE...
The Genomics Education Partnership (GEP) engages students in a course-based undergraduate research experience (CURE). To better understand the student attributes that support success this CURE, we asked about their attitudes using previously published scales measure epistemic beliefs work and science, interest grit. We found, general, bring with them into classroom contribute to two outcome measures, namely, learning as assessed by pre- postquiz perceived self-reported benefits. While GEP...
Abstract To investigate the contribution of folding cores to thermodynamic stability RNases H, we used rational design create two chimeras composed parts a thermophilic and mesophilic RNase H. Each chimera combines core from one parent protein remaining other. Both form active, well‐folded Stability curves, based on CD‐monitored chemical denaturations, show that with is more stable, has higher midpoint thermal denaturation, lower change in heat capacity (ΔCp) upon unfolding than core. A...
The kinetics of the ATP-dependent RNA−DNA helicase activity Escherichia coli transcription termination factor rho have been analyzed. Helicase substrates were assembled using 255 nt and 391 RNA sequences from trp t‘ transcript E. coli. These each carry a “loading site” at position near 5‘-end, rho-dependent terminator sequence 3‘-end to which complementary ∼20 DNA oligonucleotides annealed. A rapid (∼ 30 s) pre-steady-state burst (DNA oligomer release), followed by slow linear phase, is...
Abstract Bioinformatics, a discipline that combines aspects of biology, statistics, and computer science, is increasingly important for biological research. However, bioinformatics instruction rarely integrated into life sciences curricula at the undergraduate level. To understand why, Network Integrating Bioinformatics Life Sciences Education (NIBLSE, “nibbles”) recently undertook an extensive survey faculty in United States. The responses to open-ended questions about barriers integration...
Gene structure, transcription, translation, and alternative splicing are challenging concepts for many undergraduates studying biology. These topics typically covered in a traditional lecture environment, but students often fail to master retain these concepts. To address this problem we have designed series of six Modules that employ an active learning approach using bioinformatics tool, the genome browser, help understand eukaryotic gene structure functionality. Students learn how use...
How does DNA, the molecule containing genetic information, change its three-dimensional shape during complex cellular processes of replication, recombination and repair? This is one core questions in molecular biology which cannot be answered without help from mathematical modeling. Basic concepts topology geometry can introduced undergraduate teaching to students understand counterintuitive structural transformations that occur every living cell. Topoisomerases, a fascinating class enzymes...
Abstract Bioinformatics is becoming increasingly central to research in the life sciences. However, despite its importance, bioinformatics skills and knowledge are not well integrated undergraduate biology education. This curricular gap prevents students from harnessing full potential of their education, limiting career opportunities slowing genomic innovation. To advance integration into sciences a framework core competencies needed. that end, we here report results survey faculty United...
The purpose of the meeting described in this review was to decide how best ensure sustainability <a href="https://qubeshub.org/community/groups/niblse">Network for Integrating Bioinformatics into Life Science Education</a> (NIBLSE; pronounced “nibbles”). Biology research today generates large and complex datasets, analysis these datasets is becoming increasingly critical progress field. long-term goal NIBLSE address need achieve full integration bioinformatics undergraduate life...
Scientists often model complex biological phenomena in vitro, mimicking conditions found living organisms. Understanding the power and limitations of models is an important topic undergraduate science. In this activity, students develop their own vitro for testing survival bacteria from commercial probiotic supplements. Students work groups to decide which factors are a chosen portion human digestive tract. Groups create organs such as stomach and/or intestines. expose supplement tract,...