A5078768833- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Neurobiology and Insect Physiology Research
- Sperm and Testicular Function
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Silk-based biomaterials and applications
- Reproductive Biology and Fertility
- Genetics, Bioinformatics, and Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Career Development and Diversity
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Insect Resistance and Genetics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biomedical and Engineering Education
- Genomics and Chromatin Dynamics
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Genetic and Kidney Cyst Diseases
- Carcinogens and Genotoxicity Assessment
St. John's University
1998-2020
University of Basel
2003
Case Western Reserve University
1992
Massachusetts Institute of Technology
1985-1990
IIT@MIT
1988
Boston Children's Hospital
1978
Harvard University
1978
ABSTRACT The morphogenesis of spermatids generally takes place within a syncytium, in which all spermatid nuclei descended from primary spermatocyte remain connected via an extensive network cytoplasmic bridges. A late step sperm maturation therefore requires the physical resolution or cyst, into individual cells, process sometimes referred to as individualization. Despite identification specialized machinery involved individualization Drosophila (Tokuyasu, K. T., Peacock, W. J. and Hardy,...
There is widespread agreement that science, technology, engineering, and mathematics programs should provide undergraduates with research experience. Practical issues limited resources, however, make this a challenge. We have developed bioinformatics project provides course-based experience for students at diverse group of schools offers the opportunity to tailor local curriculum institution-specific student needs. assessed both attitude knowledge gains, looking insights into how respond...
The clathrin heavy chain (HC) is the major structural polypeptide of cytoplasmic surface lattice clathrin-coated pits and vesicles. As a genetic approach to understanding role in cellular morphogenesis developmental signal transduction, (Chc) gene Drosophila melanogaster has been identified by combination molecular classical approaches. Using degenerate primers based on mammalian yeast HC sequences, small fragment was amplified from genomic DNA polymerase reaction. Genomic cDNA clones phage...
Summary— Here, we use three polyclonal anticentrin antisera designated 08/28, 26/14‐1, and 26/14‐2 to further characterize the pericentriolar lattice of metazoan cells. all these antibodies give an indistinguishable localization pattern that consists a constellation pericentrosomal spots. In QT6 cells spots are few in number closely associated with centriolar region, whereas PtK 2 they more numerous distributed from point microtubule focus. mitotic cells, centrin is localized spindle poles...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and organization of the gene 1 portal protein required for phage P22 DNA packagingChristopher Bazinet, Julyet Benbasat, Jonathan King, Jose Maria Carazo, L. CarrascosaCite this: Biochemistry 1988, 27, 6, 1849–1856Publication Date (Print):March 22, 1988Publication History Published online1 May 2002Published inissue 22 March 1988https://pubs.acs.org/doi/10.1021/bi00406a009https://doi.org/10.1021/bi00406a009research-articleACS...
A hallmark of the research experience is encountering difficulty and working through those challenges to achieve success. This ability essential being a successful scientist, but replicating such in teaching setting can be difficult. The Genomics Education Partnership (GEP) consortium faculty who engage their students genomics Course-Based Undergraduate Research Experience (CURE). Students participate genome annotation, generating gene models using multiple lines experimental evidence. Our...
Axonemes are microtubule-based organelles of crucial importance in the structure and function eukaryotic cilia flagella. Despite great progress understanding how axonemes assembled, signals that initiate axoneme outgrowth remain unknown. Here, we identified phosphatidylinositol phosphates (phosphoinositides) as key regulators early stages Drosophila melanogaster spermatogenesis. In a study 4,5-bisphosphate [PtdIns(4,5)P2] developing male germ cells, depleted PtdIns(4,5)P2 by expression...
Research Articles| May 06 2008 Expression of ACONs and GALT in man-rodent somatic cell hybrids Subject Area: Genetics G.A.P. Bruns; Bruns Division Clinical Genetics, Children's Hospital Medical Center, Department Pediatrics, Harvard School, Boston, Mass. Search for other works by this author on: This Site PubMed Google Scholar A.C. Leary; Leary R.E. Eisenman; Eisenman C.W. Bazinet; Bazinet V.M. Regina; Regina P.S. Gerald Cytogenetics Cell (1978) 22 (1-6): 172–176....
Summary Although it is generally accepted that mitochondria and chloroplasts are descended in evolution from bacteria, the potential contributions of their endosymbiont ancestors to specialized cellular pathways development remain largely unexplored. Here we show a motile behavior Drosophila spermiogenesis strikingly similar actin‐based “comet tail” motility several bacteria. A combination electron fluorescence microscopy demonstrates major reorganization movement ahead of, close association...
The SLC6 class of membrane transporters, known primarily as neurotransmitter is increasingly appreciated for its roles in nutritional uptake amino acids and other developmentally specific functions. A Drosophila gene, Neurotransmitter transporter-like (Ntl), expressed only the male germline. Mobilization a transposon inserted near 3′ end Ntl coding region yields male-sterile mutants defining single complementation group. Germline transformation with cDNAs under control germline-specific...
Individual sperm cells are resolved from a syncytium during late step of spermiogenesis known as individualization, which is accomplished by an Individualization Complex (IC) composed 64 investment cones. mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting role for microtubule dynamics in individualization. Indeed, population ∼100 cytoplasmic microtubules fails to disappear mutant testes spermatogenesis. This persistence, detected using epi-fluorescence and electron microscopy,...
Because mammalian light chains have been implicated in the regulation of clathrin coat assembly and neuronal specialization clathrin-mediated vesicle trafficking, a light-chain gene Drosophila has sought as genetically tractable model for these developmental membrane-trafficking systems. A identified its expression examined various stages tissues by reverse transcriptase-polymerase chain reaction (RT-PCR). cDNA clone, originally from partial sequence Berkeley Genome Project EST database,...
ABSTRACT Ciliary stability and function are regulated by the covalent addition of polyglutamate polyglycine chains to axonemal tubulin subunits. The Drosophila gene CG13442 encodes a predicted ubiquitin E3 ligase involved in regulation glycylation glutamylation. Homologous mammalian MARCH8, CG13442/ dMARCH8 is required for male fertility. Sperm mutant testes appear undergo normal individualization process but fail be transferred seminal vesicle. This phenotype very similar that mutants Ntl...