A5018513661- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Silk-based biomaterials and applications
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Hemispheric Asymmetry in Neuroscience
- Cellular transport and secretion
- Protist diversity and phylogeny
- Genetics, Aging, and Longevity in Model Organisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Molecular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Insect Resistance and Genetics
- Retinal Development and Disorders
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Morphological variations and asymmetry
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
Ehime University
2025
Osaka University
2015-2024
Kogakuin University
2021
Kanazawa University
2019
Google (United States)
2016
Tokyo University of Science
2005-2015
Shinshu University
2008
Ōtani University
2008
Japan Science and Technology Agency
1999-2007
University of Shizuoka
2007
ABSTRACT We present a molecular and genetic analysis which elucidates the role of deltex in Notch signaling pathway. Using yeast ‘interaction trap’ assay, we define protein regions responsible for heterotypic interactions between Deltex intracellular domain as well uncover homotypic interaction among molecules. The function Deltex-Notch domains is examined by vivo expression studies. Taken together, data from overexpression fragments studies physical Notch, suggest that positively regulates...
E47 is a widely expressed transcription factor that activates B-cell-specific immunoglobulin gene and required for early B-cell development. In an effort to identify processes regulate E47, potentially development, we found activated Notch1 Notch2 effectively inhibit activity. Only the intact protein was inhibited by Notch-fusion proteins containing isolated DNA binding activation domains were unaffected-suggesting Notch targets atypical cofactor. Although overexpression of coactivator p300...
Some organs in animals display left-right (LR) asymmetry. To better understand LR asymmetric morphogenesis Drosophila, we studied directional rotation of the hindgut epithelial tube. Hindgut cells adopt a (chiral) cell shape within their plane, and refer to this behavior as planar cell-shape chirality (PCC). Drosophila E-cadherin (DE-Cad) is distributed boundaries with asymmetry, which responsible for PCC formation. Myosin ID switches polarity found DE-Cad distribution, coincides direction...
ABSTRACT Genetic data from Drosophila have suggested a functional relationship between the novel cytoplasmic protein encoded by deltex locus and transmembrane receptor Notch. We demonstrated direct interaction these proteins expression studies conducted in cultured cells, yeast, imaginal wing disc. binds specifically to Notch ankyrin repeats, region that is crucial for signaling constitutes most conserved domain among family members. In addition, we present new allele, Nsu42c, associated...
Intercellular signaling through the cell-surface receptor Notch plays important roles in a variety of developmental processes as well pathogenesis some human cancers and genetic disorders. However, mechanisms by which signals are transduced into cells still remain elusive. Here we investigated for cell fate control neural progenitor cells. We show that Deltex-1 (DTX1), mammalian homolog of<i>Drosophila</i> Deltex, mediates signal to block differentiation found significant fraction DTX1...
Notch (N) signaling is an evolutionarily conserved mechanism that regulates many cell-fate decisions. deltex (dx) encodes E3-ubiquitin ligase binds to the intracellular domain of N and positively signaling. However, precise Dx action unknown. Here, we found was required sufficient activate expression gene targets canonical Su(H)-dependent pathway. Although a cis-acting element overlaps with Su(H)-binding site, activated target enhancer signaling, dorsoventral compartment boundary vestigial...
Notch signalling, which is highly conserved from nematodes to mammals, plays crucial roles in many developmental processes. In the Drosophila embryo, deficiency signalling results neural hyperplasia, commonly referred as neurogenic phenotype. We identify a novel maternal gene, neurotic, and show that it essential for signalling. neurotic encodes homolog of mammalian GDP-fucose protein O-fucosyltransferase, adds fucose sugar epidermal growth factor-like repeats known play role functions...
Mastermind (Mam) has been implicated as an important positive regulator of the Notch signaling pathway by genetic studies usingDrosophila melanogaster. Here we describe a biochemical mechanism action Mam within pathway. Expression human sequence related to Drosophila (hMam-1) in mammalian cells augments induction Hairy Enhancer split (HES) promoters signaling. hMam-1 stabilizes and participates DNA binding complex intracellular domain Notch1 CSL protein. Truncated versions that can maintain...
ABSTRACT The Notch (N) pathway defines an evolutionarily conserved cell signaling mechanism that governs fate choices through local interactions. ankyrin repeat region of the receptor is essential for and has been implicated in interactions between two intra-cellular elements pathway: Deltex (Dx) Sup-pressor Hairless (Su(H)). Here we examine directly function cdc10/ankyrin repeats (ANK repeats) by transgenic biochemical analysis. We present evidence implicating ANK regulation homotypic In...
The Notch pathway is an evolutionarily conserved signaling mechanism that essential for cell-cell interactions. Drosophila deltex gene regulates in a positive manner, and its product physically interacts with the intracellular domain of through N-terminal domain. Deltex has two other domains are presumably involved protein-protein interactions: proline-rich motif binds to SH3-domains, RING-H2 finger motif. Using overexpression assay, we have analyzed functional involvement these signaling....
Notch is a transmembrane receptor that mediates the cell-cell interactions necessary for many cell-fate decisions. Endocytic trafficking of plays important roles in activation and downregulation this receptor. A Drosophila O-FucT-1 homolog, encoded by O-fut1, catalyzes O-fucosylation Notch, modification essential signaling ligand binding. It was recently proposed O-fut1 acts as chaperon endoplasmic reticulum required to exit reticulum. Here, we report has additional functions endocytic...
One of the most pressing challenges in cell-free synthetic biology is to assemble well-controlled genetic circuits. However, no complex circuits have been reported eukaryotic systems, unlike case bacterial ones, despite several unique advantages former. We here developed protein-responsive upregulating riboswitches (ON-riboswitches) that function wheat germ extract create multistep gene regulatory cascades. Although initial two types ON-riboswitches we first designed were less efficient than...
The myosin superfamily comprises over 70 classes, each with multiple subclasses, and shows substantial diversity in properties such as velocity, ATPase activity, duty ratio, directionality. This functional underpins the specialized roles of various organisms, organs, cell types. Recent studies have revealed that certain myosins induce chiral curved motions actin filaments. However, this newly identified property remains largely unexplored. Here, we investigated motion vitro using Chara...