A5040132475- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Iron Metabolism and Disorders
- Bacteriophages and microbial interactions
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Plant Micronutrient Interactions and Effects
- Cassava research and cyanide
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Trace Elements in Health
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Cystic Fibrosis Research Advances
Miami University
2015-2024
Administración Nacional de Laboratorios e Institutos de Salud
2023
Case Western Reserve University
2019
Kyiv City Clinical Oncology Center
2019
Universidade de Santiago de Compostela
2016
Georgia Institute of Technology
2011
Pearson (United States)
1998-2009
Oregon Health & Science University
1985-1995
Universidad Nacional de Córdoba
1983-1994
University of Tübingen
1991
Acinetobacter baumannii causes severe infections in compromised patients, survives on abiotic surfaces hospital environments and colonizes different medical devices. In this study the analysis of processes involved surface attachment biofilm formation by prototype strain 19606 was initiated. This attaches to forms structures plastic glass surfaces, particularly at liquid–air interface cultures incubated stagnantly. The cell aggregates, which contain stacks separated water channels, formed...
ABSTRACT The ability of Acinetobacter baumannii to adhere and persist on surfaces as biofilms could be central its pathogenicity. production pili a biofilm-associated protein the expression antibiotic resistance are needed for robust biofilm formation abiotic biotic surfaces. This multistep process also depends transcriptional regulatory functions, some which sense nutrients available cells. report extends previous observations by showing that although outer membrane A (OmpA) A. 19606 plays...
Acinetobacter baumannii is a Gram-negative opportunistic nosocomial pathogen. This microorganism survives in hospital environments despite unfavorable conditions such as desiccation, nutrient starvation and antimicrobial treatments. It hypothesized that its ability to persist these environments, well virulence, result of capacity form biofilms. A. forms biofilms on abiotic surfaces polystyrene glass biotic epithelial cells fungal filaments. Pili assembly production the Bap surface-adhesion...
Acinetobacter baumannii is recognized as an emerging bacterial pathogen because of traits such prolonged survival in a desiccated state, effective nosocomial transmission, and inherent ability to acquire antibiotic resistance genes. A pressing need the field A. research suitable model strain that representative current clinical isolates, highly virulent established animal models, can be genetically manipulated. To identify strain, diverse set recent U.S. military isolates was assessed....
Acinetobacter baumannii , which causes serious infections in immunocompromised patients, expresses high-affinity iron acquisition functions needed for growth under iron-limiting laboratory conditions. In this study, we determined that the initial interaction of ATCC 19606 T type strain with A549 human alveolar epithelial cells is independent production BasD and BauA, proteins acinetobactin biosynthesis transport, respectively. contrast, these are required to persist within cause their...
Significance The Science Education Alliance–Phage Hunters Advancing Genomics and Evolutionary program is an inclusive Research Community with centralized programmatic scientific support, in which broad student engagement authentic science linked to increased accessibility research experiences for students; persistence of these students science, technology, engineering, mathematics; productivity faculty alike.
Acinetobacter baumannii forms biofilms on abiotic surfaces, a phenotype that may explain its ability to survive in nosocomial environments and cause device-related infections compromised patients. The biofilm proficiency of the 19606 type strain depends production pili, cell-surface appendages assembled via CsuAB-A-B-C-D-E chaperone-usher secretion system. screening bank isogenic insertion derivatives led identification biofilm-deficient derivative which transposon disrupted gene predicted...
Acinetobacter baumannii is a Gram-negative, opportunistic pathogen. Recently, multiple A. genomes have been sequenced; these data led to the identification of many genes predicted encode proteins required for biogenesis type IV pili (TFP). However, there no experimental evidence demonstrating that strains actually produce functional TFP. Here, we demonstrated strain M2 naturally transformable and capable twitching motility, two classical TFP-associated phenotypes. Strains were constructed...
ABSTRACT Light is a ubiquitous environmental signal that many organisms sense and respond to by modulating their physiological responses accordingly. While this an expected response among phototrophic microorganisms, the ability of chemotrophic prokaryotes react light has become puzzling novel issue in bacterial physiology, particularly pathogens. In work, we show opportunistic pathogen Acinetobacter baumannii senses responds blue light. Motility formation biofilms pellicles were observed...
Anguibactin, a siderophore produced by cells of Vibrio anguillarum 775 harboring the pJM1 plasmid, has now been isolated from supernatants iron-deficient cultures. This iron-reactive material was purified adsorption onto an XAD-7 resin and subsequent gel filtration on Sephadex LH-20 column. The resulting neutral compound ion at m/z 348 in mass spectrometry contained one sulfur, four oxygen, nitrogen atoms as determined elemental analysis. Its strong UV absorbance blue fluorescence were...
Multiple species within the Acinetobacter genus are nosocomial opportunistic pathogens of increasing relevance worldwide. Among virulence factors utilized by these bacteria type IV pili and a protein O-glycosylation system. Glycosylation is mediated O-oligosaccharyltransferases (O-OTases), enzymes that transfer glycan from lipid carrier to target proteins. difficult identify due similarities with WaaL ligases catalyze last step in lipopolysaccharide synthesis. A bioinformatics analysis...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of anguibactin, a unique plasmid-related bacterial siderophore from the fish pathogen Vibrio anguillarumM. A. F. Jalal, M. B. Hossain, Dick Van der Helm, J. Sanders-Loehr, L. Actis, and H. CrosaCite this: Am. Chem. Soc. 1989, 111, 1, 292–296Publication Date (Print):January 1989Publication History Published online1 May 2002Published inissue 1 January...
The Acinetobacter baumannii type strain, ATCC 19606, secretes acinetobactin, a catechol siderophore highly related to the iron chelator anguibactin produced by fish pathogen Vibrio anguillarum ( Listonella ). This paper reports initial characterization of genes and gene products involved in acinetobactin-mediated iron-acquisition process. Insertional mutagenesis resulted isolation several derivatives whose ability grow medium containing 2,2′-dipyridyl was affected. One insertions disrupted...
Vibrio anguillarum 775 harboring the virulence plasmid pJM1 synthesized an outer membrane protein of 86 kilodaltons, OM2, that was inducible under conditions iron limitation. DNA fragments obtained by digestion with restriction endonucleases were cloned into cosmid vectors and transferred Escherichia coli. The OM2 in E. coli, demonstrating it is actually encoded plasmid. Mobilization recombinant plasmids to V. accomplished using transfer factor pRK2013. A exconjugant derivative pJHC-T7...
ABSTRACT Acinetobacter baumannii is an opportunistic pathogen that causes severe nosocomial infections. Strain ATCC 19606 T utilizes the siderophore acinetobactin to acquire iron under iron-limiting conditions encountered in host. Accordingly, genome of this strain has three tonB genes encoding proteins for energy transduction functions needed active transport nutrients, including iron, through outer membrane. Phylogenetic analysis indicates these genes, which are present genomes all...
Acinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound urinary tract infections. Analysis A. representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated this increasingly to sheep, human horse erythrocytes, which interestingly contain increasing amounts phosphatidylcholine their membranes. Bioinformatic, genetic functional analyses 19...
Abstract Complementation of insertion mutants showed that the polypeptides FatD, FatC, FatB, and FatA are essential for iron-transport process encoded by pJM1. Sequence analysis followed homology studies indicated transport ferric anguibactin into Vibrio anguillarum 775 follows same mechanism as reported Fe(3+)-hydroxamates, Fe(3+)-catecholates, dicitrate, vitamin B12 Escherichia coli. Homology FatA, part receptor complex, to seven E. coli proteins involved in uptake siderophores supports...
The iron-transport genes from the pJMl plasmid of Vibrio anguillarum have been cloned and sequenced.Five open-reading frames identified, one which encodes outer membrane receptor for ferric anguibactin, OM2.This coding region corresponds to a protein 726 amino acids with Mr 78,777.The has hydrophobic signal sequence 36 potential membrane-associated at carboxyl terminus.A 2.3-kilobase iron-regulated mRNA was transcribed this in vivo.The four other were shown be involved regulation OM2...