A5077670541- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Plant Pathogenic Bacteria Studies
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Microbial Community Ecology and Physiology
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Genetics and Biotechnology
- Urinary Tract Infections Management
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Bacillus and Francisella bacterial research
- Probiotics and Fermented Foods
- Nosocomial Infections in ICU
- Identification and Quantification in Food
- Microbial Natural Products and Biosynthesis
- Antibiotics Pharmacokinetics and Efficacy
Leiden University Medical Center
2012-2024
Leiden University
1997-2013
University of Cologne
1997-2008
Amsterdam UMC Location University of Amsterdam
2005
University of Amsterdam
2005
Osaka University
2005
Scripps Research Institute
2005
National Institute of Public Health
2002-2004
Queen's Medical Centre
2004
Institut Scientifique de Santé Publique
2000
Outbreaks of hospital infections caused by multidrug resistant Acinetobacter baumannii strains are increasing concern worldwide. Although it has been reported that particular outbreak geographically widespread, little is known about the diversity and phylogenetic relatedness A. clonal groups. Sequencing internal portions seven housekeeping genes (total 2,976 nt) was performed in 154 covering breadth including representatives previously recognized international clones, 19 other species....
ABSTRACT A standard procedure for pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments Acinetobacter baumannii was set up and validated its interlaboratory reproducibility potential use in the construction an Internet-based database international monitoring epidemic strains. The PFGE fingerprints strains were generated at three different laboratories with ApaI as restriction enzyme by a rigorously standardized procedure. results analyzed respective also centrally national...
The naming of prokaryotes is governed by the International Code Nomenclature Prokaryotes (ICNP) and partially for Algae, Fungi Plants (ICN). Such codes must be able to determine names taxa in a universal unambiguous manner, thus serving as common language across different fields activities. This unity undermined when new code nomenclature emerges that overlaps scope with an established, time-tested uses same format but assigns nomenclatural status values names. resulting confusion not...
Thirty-one Acinetobacter baumannii strains, comprising 14 strains from outbreaks in different northwestern European cities and 17 sporadic were compared by investigating various properties of the including biotype, antibiogram, cell envelope protein electrophoretic profile, ribotype pattern, band pattern generated a novel genomic fingerprinting method, named AFLP, which is based on selective amplification restriction fragments. Results showed that 12 unrelated linked together two clusters...
At least 19 genomic species are recognized as constituting the genus Acinetobacter. However, little is known about natural reservoirs of various members genus. An epidemiological study was therefore performed to investigate colonization with Acinetobacter spp. skin and mucous membranes 40 patients hospitalized in a cardiology ward healthy controls. Single samples were obtained once from each nine different body sites, i.e., forehead, ear, nose, throat, axilla, hand, groin, perineum, toe web....
A total of 53 field and reference strains, including the type strains seven named species (nomenspecies) belonging to 18 described genomic (DNA groups) genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA). Restriction with enzymes AluI, CfoI, MboI, RsaI, MspI enzymatically 16S rRNA genes allowed us identify all except 4 (Acinetobacter haemolyticus) 7 (A. johnsonii), 5 junii) 17, 10 11, which clustered pairwise in three respective groups. Further enzyme...
ABSTRACT The species Acinetobacter calcoaceticus , A. baumannii genomic 3, and 13TU included in the - complex are genetically highly related difficult to distinguish phenotypically. Except for they all important nosocomial species. In present study, usefulness of 16S-23S rRNA gene intergenic spacer (ITS) sequence differentiation (genomic) was evaluated. ITSs 11 reference strains 17 other were sequenced. ITS lengths (607 638 bp) sequences conserved within complex. Intraspecies similarities...
Seven laboratories in six European countries examined 40 isolates belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex investigate whether standardized protocols and quality-controlled reagents could produce reliable, discriminatory, reproducible PCR-based fingerprinting results. Four PCR with different primers (primers DAF4, ERIC-2, M13, REP1 + REP2) were used. The epidemiological conclusions reached by participating substantially correct, 96.4% of total isolate...
Bacteria belonging to the genus Acinetobacter are ubiquitous in soil and water. Only a few species, including baumannii, unnamed genomic species (gen. sp.) 3 13TU, which together with organism calcoaceticus combined A. calcoaceticus-A. baumannii (Acb) complex, have been recognized as important nosocomial infectious agents. The ecology, epidemiology pathology of most not yet well established. Lack practical accurate methods limits routine identification clinical isolates thus hampers precise...
An understanding of why certain Acinetobacter species are more successful in causing nosocomial infections, transmission and epidemic spread healthcare institutions compared with other is lacking. We used genomic, phenotypic virulence studies to identify differences between species. Fourteen strains representing nine were examined. Genomic analysis six showed that the A. baumannii core genome contains many genes important for diverse metabolism survival host. Most also present one or less...
Acinetobacter baumannii is an opportunistic bacterial pathogen that increasingly associated with gram-negative nosocomial pneumonia, but the molecular mechanisms play a role in innate defenses during A. infection have not been elucidated.To gain first insight into of CD14 and Toll-like receptors 4 2 host response to pneumonia.Respective gene-deficient mice were intranasally infected baumannii, outgrowth, lung inflammation, pulmonary cytokine/chemokine responses determined. To study...
The taxonomic status of 24 haemolytic, non-glucose acidifying Acinetobacter strains that did not belong to any previously described species was investigated by means a polyphasic approach. Using AFLP fingerprinting, amplified rDNA restriction analysis and phenotypic characterization, the were classified into two phenetically coherent groups (comprising 15 9 strains) distinct from each other all known species. Confirmation these formed separate lineages within genus obtained comparative...
The aim of this study was to analyse the emergence carbapenem resistance among hospital strains Acinetobacter in Czech Republic.Acinetobacter isolates were collected prospectively 2005-06 from 19 diagnostic laboratories. They identified species level by AFLP, typed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing, tested for susceptibility 14 antimicrobials presence 20 genes associated with antimicrobial resistance.A total 150 obtained 56 intensive care units...
We have sequenced the gene clusters for type strains of Acinetobacter baumannii serotyping scheme developed in 1990s, and used sequences to better understand diversity surface polysaccharides genus. obtained genome 27 available serovar strains, identified 25 polysaccharide cluster sequences. There are structures 12 these polysaccharides, general genes present appropriate structure where known. This greatly facilitates interpretation. also find 53 different glycosyltransferase genes, 7 can...
Following characterisation by phenotypic tests and amplified ribosomal DNA restriction analysis (ARDRA), 50 tetracycline-resistant (MIC≥165mumg/L) Acinetobacter strains from clinical (n=35) aquatic (n=15) samples were analysed PCR for tetracycline resistance (Tet) determinants of classes A–E. All the A. baumannii; most (33 35) had Tet A (n=16) or B (n=17) determinants, only two did not yield amplicons with primers any five determinants. The belonged to genomic species other than baumannii,...
The taxonomic status of two recently described phenetically distinctive groups within the genus Acinetobacter, designated phenon 1 and 2, was investigated further. study collection included 51 strains, mainly clinical origin, from different European countries with properties either (29 strains) or 2 (22 strains). DNA-DNA hybridization studies DNA polymorphism analysis by AFLP revealed that these phenons represented new genomic species. Furthermore, 16S rRNA gene sequence three...
AFLP is a novel genomic fingerprinting method based on the selective PCR amplification of restriction fragments. The usability this for differentiation species in genus Acinetobacter was investigated. A total 151 classified strains (representing 18 species, including type, reference, and field strains) 8 unclassified were analyzed. By using single set enzymes (HindIII TaqI) one particular primers, all could be allocated to correct groups properly separated, with minimal intraspecific...
In the last few years, phenotypically carbapenem resistant Acinetobacter strains have been identified throughout world, including in many of hospitals and intensive care units (ICUs) Australia. Genotyping Australian ICU outbreak-associated isolates by pulsed-field gel electrophoresis whole genomic DNA indicated that different were cocirculating within one hospital. The carbapenem-resistant phenotype these other was found to be due carbapenem-hydrolyzing activity associated with presence...