A5006042983- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Genetics, Bioinformatics, and Biomedical Research
- Science Education and Pedagogy
- Innovative Teaching Methods
- 3D Printing in Biomedical Research
- Marine Ecology and Invasive Species
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Education and Critical Thinking Development
- Planarian Biology and Electrostimulation
- Neonatal Respiratory Health Research
- HIV Research and Treatment
- Immune Response and Inflammation
- Biomedical and Engineering Education
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- Experimental Learning in Engineering
- Innovative Teaching and Learning Methods
- Epigenetics and DNA Methylation
- Career Development and Diversity
- Educational Strategies and Epistemologies
- Marine Invertebrate Physiology and Ecology
University of California, Merced
2011-2024
Stanford University
1997-2021
Institut Pasteur
2005-2013
Inserm
2006-2013
Babraham Institute
2005
Center for Rheumatology
1998-2003
Sorted CD4+ and CD8+ T cells from the peripheral blood or bone marrow of donor C57BL/6 (H-2b) mice were tested for their capacity to induce graft-versus-host disease (GVHD) by injecting cells, along with stringently cell–depleted into lethally irradiated BALB/c (H-2d) host mice. The at least 30 times more potent than in inducing lethal GVHD. As NK1.1+ represented <1% all ∼30% marrow, sorted NK1.1− GVHD was tested. latter had markedly increased potency, adding back suppressed secreted...
Apoptosis of peripheral blood T cells has been suggested to play an important role in the pathogenesis human immunodeficiency virus (HIV) infection. Spontaneous, Fas (CD95)–induced and activation-induced cell apoptosis have all described mononuclear cultures HIV-infected individuals. We previously shown that is independent from HIV+ In this study, we extend confirm these observations by using inhibitor interleukin-1β converting enzyme (ICE) homologues. show z-VAD-fmk, a tripeptide ICE...
Nano- and microscale topographical cues play critical roles in the induction maintenance of various cellular functions, including morphology, adhesion, gene regulation, communication. Recent studies indicate that structure function at heart tissue level is exquisitely sensitive to mechanical nano-scale as well level. Although fabrication methods exist for generating features cell culture, current techniques, especially those with nanoscale resolution, are typically complex, prohibitively...
We describe the development and validation of a new instrument, Classroom Discourse Observation Protocol (CDOP), which quantifies teacher discourse moves (TDMs) from observational data in undergraduate STEM classrooms. TDMs can be conceptualized as epistemic tools that mediate classroom discussions. Through an inductive–deductive coding process, we identified commonly occurring among group biology instructors (n = 13, 37 class session) teaching Active Learning Environments. CDOP scheme its...
Increasingly, curricular materials for undergraduate life science courses are designed to highlight scientists with identities and backgrounds that counter historical stereotypical representation in science. In this essay, we characterize the wide variation development implementation of these featuring counterstereotypical scientists. Applying Social Ecological Model Behavior Change as a framework, examine both personal social elements benefits costs related designing implementing curricula...
Human T lymphocytes were stimulated using phorbol myristate acetate and ionomycin. Twenty-four hours post-activation the cells harvested for DNA content measurements a newly developed cell profiling system employing dielectrophoresis. This provides individual size dielectrophoresis data statistically relevant numbers of control activated cells. From this it was determined that mean membrane specific capacitance decreased from 13.49 (+/- 4.72) mF/m(2) to 10.62 5.13) mF/m(2). can be related...
Target of Rapamycin (TOR) controls an evolutionarily conserved signaling pathway that modulates cellular growth and division by sensing levels nutrients, energy stress. As such, TOR is a crucial component tissues organs translates systemic signals into behavior. The ubiquitous nature signaling, together with the difficulty analyzing tissue during turnover repair, have limited our understanding how this kinase operates throughout body. Here, we use planarian model system to address regulation...
We report a facile and scalable method to fabricate biomimetic giant liposomes by using cellulose paper-based materials platform. Termed PAPYRUS for Paper-Abetted liPid hYdRation in aqUeous Solutions, the is general can produce various aqueous media at elevated temperatures. Encapsulation of macromolecules production with membranes complex compositions straightforward. The ease manipulation paper makes practical massive parallelization scale-up fabrication liposomes, demonstrating first time...
T cell apoptosis has been proposed as an Important contributor to the functional defects and depletion of cells in HIV-lnfected Individuals. However, mechanisms Involved this have not elucidated. We recently showed that peripheral blood from HIV-infected individuals are especially susceptible Fas antigen-induced apoptosis. In study we examine role Fas, CTLA-4, tumor necrosis factor (TNF) receptors (TNFR) CD30, known be involved activation-induced death (AICD), spontaneous activation...
In the principal pathway of α/β T cell maturation, precursors from bone marrow migrate to thymus and proceed through several well-characterized developmental stages into mature CD4+ CD8+ cells. This study demonstrates an alternative in which microenvironment also supports differentiation The recapitulates thymic maturation including a CD4+CD8+ intermediary positive negative selection, is strongly inhibited by presence contribution vivo requires further mice with normal deficient or immune function.
Abstract Reform efforts in undergraduate science, technology, engineering, and mathematics (STEM) instruction often emphasize student-centered teaching approaches, but relatively little attention is paid to the way STEM teachers use discourse when interacting with their students. In present study, we examined instructional behaviors of biology faculty members (N = 20) classes. Although found that spent most time guiding student learning active activities less presenting, an analysis...
Abstract Introduction Hematopoietic stem cells (HSCs) follow a genetically programmed pattern of migration during development. Extracellular matrix and adhesion molecules, as well chemokines their receptors, are important in adult HSC migration. However, little is known about the role these molecules play at earlier developmental stages. Methods We have analyzed by quantitative polymerase chain reaction (qPCR) array expression extracellular chemokine receptors Lineage - Sca-1 + c-Kit (LSK)...
National efforts to improve equitable teaching practices in biology education have led an increase research on the barriers student participation and performance, as well solutions for overcoming these barriers. Fewer studies examined extent which resulting data trends effective strategies are generalizable across multiple contexts or specific individual classrooms, institutions, geographic regions. To address gaps our understanding, establish baseline information about students contexts, a...
We searched for clonable committed T cell progenitors in the adult mouse bone marrow and isolated rare (≈0.05%) cells with Thy-1 hi CD2 − CD16 + CD44 CD25 Lin phenotype. In vivo experiments showed that these were only to reconstituting lineage of irradiated Ly5 congenic hosts. Reconstitution thymus was minimal compared marrow, spleen, lymph nodes. At limiting dilutions, donor reconstitution spleen frequently occurred without detectable thymus. Progenitors capable rapidly athymic conclusion,...
Abstract We identified committed T cell progenitors (CTPs) in the mouse bone marrow that have not rearranged TCRβ gene; express a variety of genes associated with commitment to lineage, including GATA-3, cell-specific factor-1, Cβ, and Id2; show surface marker pattern (CD44+CD25−CD24+CD5−) is similar earliest thymus. More mature intermediate TCR gene loci, Vα Vβ as well CD3ε, but do or CD3 receptors. CTPs, from thymus, reconstituted αβ cells lymphoid tissues athymic nu/nu mice. These...
Planarians possess remarkable stem cell populations that continuously support cellular turnover and are instrumental in the regeneration of tissues upon injury. Cellular tissue planarians rely on proper integration local systemic signals regulate proliferation death. Thus, understanding controlling death could provide valuable insights for maintenance adult body homeostasis biology regeneration. Flow cytometry techniques have been utilized widely to identify, isolate, characterize planarian...
Background Long-term hematopoietic stem cells (LT-HSCs) migrate from the fetal liver (FL) to bone marrow (FBM) during development. Various adhesion and chemotactic receptor genes have been implicated in migration of adult LT-HSCs. However, their role LT-HSCs is not clearly understood due, part, rare number these tissues, which preclude classical gene expression analysis. The aim this study characterize related LT-HSC across different anatomical locations Methodology/Principal Findings We...
Background. Previous studies showed that a combination of posttransplant total lymphoid irradiation (TLI), rabbit antithymocyte globulin (ATG), and single donor blood transfusion induced tolerance to ACI heart allografts in Lewis rats. All three modalities were required achieve tolerance. The objective the current study was determine subset(s) cells facilitated long-term allograft survival. Methods. hosts received TLI, ATG, cell infusion after transplantation. Graft survival, mixed leukocyte...
The COVID-19 shutdown forced many institutions of higher education to shift in-person teaching emergency remote teaching. This was particularly challenging for laboratory courses, where students are expected learn hands-on skills needed their career goals.