A5061423432- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Pluripotent Stem Cells Research
- bioluminescence and chemiluminescence research
- Calcium signaling and nucleotide metabolism
- MicroRNA in disease regulation
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- CRISPR and Genetic Engineering
- Ion channel regulation and function
- Physiological and biochemical adaptations
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Renal and related cancers
- Cellular transport and secretion
- Pancreatic function and diabetes
- Retinal Development and Disorders
- Adenosine and Purinergic Signaling
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Biomedical Ethics and Regulation
Hong Kong University of Science and Technology
2015-2024
University of Hong Kong
2015-2024
Florida Fish and Wildlife Conservation Commission
2021
City University of Hong Kong
2018
Chinese University of Hong Kong
1995-2017
Catabasis Pharmaceuticals (United States)
2017
Chattanooga State Community College
2013
Brooklyn College
2011
University of Bath
1990-2007
Oregon Health & Science University
2004-2006
Summary Evidence‐based policy requires researchers to provide the answers ecological questions that are of interest makers. To find out what those in UK, representatives from 28 organizations involved policy, together with scientists 10 academic institutions, were asked generate a list their organizations. During 2‐day workshop initial 1003 generated consulting at least 654 makers and academics was used as basis for generating short 100 significant relevance. Short‐listing decided on...
Self-renewable human pluripotent stem cells (hPSCs) serve as a potential unlimited ex vivo source of cardiomyocytes (CMs) for cell-based disease modeling and therapies. Although recent advances in directed differentiation protocols have enabled more efficient derivation hPSC-derived CMs with an efficiency ∼50%-80% final yield ∼1-20 per starting undifferentiated hPSC, these are often not readily transferrable across lines without first optimizing multiple parameters. Further, the resultant...
ABSTRACT The cloned mouse macrophage cell line, BAC1.25F, resembles primary macrophages in its dependence on colony stimulating factor-1 (CSF-1) for both viability and proliferation. Re-addition of CSF-1 to cytokine-deprived cells, which are rounded with diffusely organised F-actin, stimulates rapid spreading polarisation. Using the Dunn chemotaxis chamber movement stimulated was monitored over a 2 hour period. Cells re 1.32 nM human recombinant migrated at mean rate 7.71 µm per hour, but...
The effect of basic fibroblast growth factor on the proliferative and chemotactic response cultured rat patellar tendon fibroblasts was studied in an vitro wound closure model. In quiescent confluent culture, a uniform cell free zone, or wound, generated mechanically as wound. width zone measured at 0, 6, 12, 24 hours after injury, presence 2, 10, 50 mg/mL factor. Basic factor, concentration 10 ng/mL, significantly accelerated closure, resulting almost complete by injury. 2 enhanced...
Oscillations of cytosolic free calcium levels have been shown to influence gene regulation and cell differentiation in a variety model systems. Intercellular waves thus present plausible mechanism for coordinating cellular processes during embryogenesis. Herein we report use aequorin photon imaging microscope directly observe rhythmic series intercellular that circumnavigate zebrafish embryos over 10-h period gastrulation axial segmentation. These first appeared at about 65% epiboly...
We report that, during epiboly in zebrafish, three F-actin--based structures appear only after the blastoderm migrates past embryonic equator. They are composed of two ring-like F-actin that form at deep cell and enveloping layer margins a punctate actin band develops external yolk syncytial layer. Treatment with cytochalasin B or calcium chelator dibromo-BAPTA results disruption all these actin-based structures, leading to slowing immediate arrest epiboly, respectively, followed by failure...
The process of cleavage during the syncytial blastoderm stage Drosophila embryo was studied in fixed whole-mounts using a triple-staining technique. Plasmalemma stained with Concanavalin A conjugated to tetramethylrhodamine isothiocyanate, underlying cortical F-actin fluorescein derivative phalloidin, and nuclei 4',-6 diamidine-2-phenylindole dihydrochloride. surface caps, which overlie superficial at this stage, were found be rich as compared rest cortex. After caps formed, they extended...
Abstract In zebrafish, the role of matrix metalloproteinases (MMPs) in inflammatory phase heart regeneration following cryoinjury remains poorly understood. Here, we demonstrated an increase MMP enzymatic activity and elevated expression mmp9 mmp13 injured area (IA) hearts from as early 1 day post-cryoinjury (dpc). Treatment with broad-spectrum inhibitor, GM6001, during first week after resulted impaired regeneration, indicated by larger scar reduced numbers proliferating cardiomyocytes....
ABSTRACT Through the injection of f-aequorin (a calcium-sensitive bioluminescent reporter) into dorsal micromeres 8-cell stage Xenopus laevis embryos, and use a Photon Imaging Microscope, distinct patterns calcium signalling were visualised during gastrulation period. We present results to show that localised domains elevated observed exclusively in anterior part ectoderm, these transients increased number amplitude between stages 9 11, just prior onset neural induction. During this time,...
MicroRNAs (miRNAs) are crucial for the post-transcriptional control of protein-encoding genes and together with transcription factors (TFs) regulate gene expression; however, regulatory activities miRNAs during cardiac development only partially understood. In this study, we tested hypothesis that integrative computational approaches could identify experimentally be shown to cardiomyogenesis.We integrated expression profiles bioinformatics analyses miRNA TF programs candidate involved...
Intact zebrafish embryos were used as an in vivo animal model to investigate the role of Ca2+ signaling during differentiation slow muscle cells (SMCs) within forming skeletal muscle. Transgenic generated using a-actin promoter that targeted apoaequorin expression specifically cells. Two distinct periods (CSPs) visualized developing SMCs: between ~17.5-19.5 hours post-fertilization (hpf) and after ~23 hpf, separated by a ~3.5 h quiet period. Further spatial characterization these signals...
COVID-19, resulting from infection by the SARS-CoV-2 virus, caused a contagious pandemic. Even with current vaccines, there is still an urgent need to develop effective pharmacological treatments against this deadly disease. Here, we show that water and ethanol extracts of root rhizome Polygonum cuspidatum (Polygoni Cuspidati Rhizoma et Radix), common Chinese herbal medicine, blocked entry wild-type omicron variant pseudotyped virus into fibroblasts or zebrafish larvae, IC50 values ranging...
Fibroblast growth factors (FGFs) are believed to be vital for limb outgrowth and patterning during embryonic development. Although the effect of FGFs on formation skeletal elements has been studied in detail, their development musculature is still uncertain. In this study, we used Blindwell chemotactic chambers examine FGF-2 FGF-4 motility myogenic cells obtained from proximal region day 11.5 mouse forelimbs. The were found chemotactically attracted at 1–50 ng/ml. Both increased cell...
Through the injection of f ‐aequorin (a calcium‐specific luminescent reporter), and use an imaging photon detector, transient localized elevations free cytosolic calcium in forming blastodisc (BD) animal hemisphere cortex were visualized that correlated with ooplasmic segregation. The introduction appropriate concentration weak (K D = 1.5 μmol/L) buffer 5,5′‐dibromo‐BAPTA results dissipation these domains, inhibits cytoplasmic streaming subsequent formation a BD at pole. These inhibitory...