A5030214799- Acoustic Wave Phenomena Research
- Aerodynamics and Acoustics in Jet Flows
- Genetic and Kidney Cyst Diseases
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Speech and Audio Processing
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Manufacturing Process and Optimization
- Advanced machining processes and optimization
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Biomedical Research and Pathophysiology
- Robotic Path Planning Algorithms
- Seismic Waves and Analysis
- Structural Health Monitoring Techniques
- Microtubule and mitosis dynamics
- UAV Applications and Optimization
- Robotics and Sensor-Based Localization
- Underwater Acoustics Research
- Receptor Mechanisms and Signaling
- Congenital Heart Disease Studies
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- CRISPR and Genetic Engineering
State Grid Corporation of China (China)
2016-2024
Anhui University
2018-2024
University of Hong Kong
2009-2023
Anhui Jianzhu University
2022
Hefei University of Technology
2014-2021
HKU-Pasteur Research Pole
2019-2020
China-Japan Friendship Hospital
2014-2020
Inner Mongolia Medical University
2019
Icahn School of Medicine at Mount Sinai
1999-2017
Chinese University of Hong Kong
2017
The Brownian motion of molecules at thermal equilibrium usually has a finite correlation time and will eventually be randomized after long delay time, so that their displacement follows the Gaussian statistics. This is true even when have experienced complex environment with time. Here, we report lateral acetylcholine receptors on live muscle cell membranes does not follow statistics for normal diffusion. From careful analysis large volume protein trajectories obtained over wide range...
Primary cilia play a key role in the pathogenesis of autosomal dominant polycystic kidney disease (ADPKD). The affected proteins, polycystin-1 (PC1) and polycystin-2 (PC2), interact with each other are expressed cilia. We found that COOH-terminal truncated PC2 (PC2-L703X), lacking PC1 interaction region, still traffics to examined expression several tissues cells is independently PC1. used N-terminal deletion constructs narrow domain necessary for trafficking first 15 amino acids identified...
Autosomal dominant polycystic kidney disease (AD-PKD) is predominantly caused by mutations of the gene PKD1, which encodes a large protein, polycystin, unknown function. A variety arterial abnormalities occur with increased prevalence in ADPKD patients. Using an antiserum against nonduplicated region polycystin immunostaining vascular smooth muscle cells was detected normal adult elastic arteries. Partial digestion tissue slices nonspecific proteases greatly enhanced this staining. Similar...
Polycystin, the product of autosomal dominant polycystic kidney disease (ADPKD) 1 gene (PKD1) is cardinal member a novel class proteins. As first step towards elucidating function polycystin and pathogenesis ADPKD, three types information were collected in current study: subcellular localization polycystin, spatial temporal distribution protein within normal tissues effects ADPKD mutations on pattern expression affected tissues. Antisera directed against synthetic peptide two recombinant...
Background MicroRNAs (miRs) negatively regulate transcription and are important determinants of normal heart development failure pathogenesis. Despite the significant knowledge gained in mouse studies, their functional roles human (h) remain elusive. Methods Results We hypothesized that miRs figure prominently cardiac differentiation differentially expressed differentiating, developing, terminally mature cardiomyocytes (CMs). As a first step, we mapped miR profiles embryonic stem cells...
Self-renewable human pluripotent stem cells (hPSCs) serve as a potential unlimited ex vivo source of cardiomyocytes (CMs) for cell-based disease modeling and therapies. Although recent advances in directed differentiation protocols have enabled more efficient derivation hPSC-derived CMs with an efficiency ∼50%-80% final yield ∼1-20 per starting undifferentiated hPSC, these are often not readily transferrable across lines without first optimizing multiple parameters. Further, the resultant...
A large number of drugs can induce prolongation cardiac repolarization and life-threatening arrhythmias. The prediction this side effect is however challenging as it usually develops in some genetically predisposed individuals with normal at baseline. Here, we describe a platform based on diverse panel induced pluripotent stem cells (iPSCs) that reproduces susceptibility to develop cardiotoxic drug response. We generated iPSC-derived cardiomyocytes from patients presenting vivo extremely low...
Polycystin-2 (PC-2) is a non-selective cation channel that, when mutated, results in autosomal dominant polycystic kidney disease. In an effort to understand the regulation of this channel, we investigated role protein phosphorylation PC-2 function. We demonstrated direct incorporation phosphate into cells and tissues found that constitutive occurs at Ser(812), putative casein kinase II (CK2) substrate domain. Ser(812) can be phosphorylated by CK2 vitro substitution S812A failure incorporate...
Autosomal dominant polycystic kidney disease (ADPKD) occurs by germline mutation in PKD1 or PKD2. Evidence of homozygous inactivation either gene human cyst lining cells as well mouse knockout models strongly supports a two-hit mechanism for formation. Discovery trans-heterozygous mutations and PKD2 minority renal cysts has led to the proposal that such also can play role In current study, we investigated disease. Pkd1+/−, Pkd2 +/− Pkd1+/− : mice, cystic lesion was mild variable with no...
In this paper, a mission planning system is presented that generates plans for group of unmanned aerial vehicles (UAVs) to provide continuous surveillance over an urban area. Given the information terrain and buildings in target area, two-stage approach employed solve problem. first stage, set camera locations called vantage generated provides complete coverage second one or several UAVs are determined collectively share their individual paths carry out duty. both stages, evolutionary...
Abstract. The expression of polycystin-1 in the vascular smooth muscle cells (VSMC) elastic and large distributive arteries suggests that some manifestations autosomal-dominant polycystic kidney disease (ADPKD) result directly from genetic defect. Intracranial aneurysms have been reported PKD2 , as well PKD1 families. To determine whether polycystin-2 is similar to polycystin-1, mRNA protein cultured pig aortic VSMC was studied immunofluorescence immunohistochemistry were used study...
PKD1, the gene that is mutated in approximately 85% of autosomal dominant polycystic kidney disease (ADPKD) cases humans, has recently been identified (Eur. PKD Consortium. Cell 77: 881-894, 1994; also, erratum 78: 1994). The longest open-reading frame PKD1 encodes polycystin, a novel 460-kDa protein contains series NH2-terminal adhesive domains (J. Hughes, C. J. Ward, B. Peral, R. Aspinwall, K. Clark, San Millan, V. Gamble, and P. Harris. Nat. Genet. 10: 151-160, 1995; Int. 81: 289–298,...
The generation of human pluripotent stem cell (hPSC)-derived ventricular progenitors and their assembly into a 3-dimensional in vivo functional heart patch has remained an elusive goal. Herein, we report the enriched pool hPSC-derived (HVPs), which can expand, differentiate, self-assemble, mature without aid any gel or matrix. We documented specific temporal window, HVPs will engraft vivo. On day 6 differentiation, were by depleting cells positive for pluripotency marker TRA-1-60 with...
We identified a developmentally regulated gene from mouse kidney whose expression is up-regulated in metanephrogenic mesenchyme cells when they are induced to differentiate epithelial during organogenesis. The deduced 70.5-kDa protein, originally named METS-1 (mesenchyme-to-epithelium transition protein with SH3 domains), has since been cloned as CD2-associated (CD2AP). CD2AP strongly expressed glomerular podocytes, and the absence of mice results congenital nephrotic syndrome. have found...
Mutations in PKD1 and PKD2, the genes that encode polycystin-1 polycystin-2 respectively, account for almost all cases of autosomal dominant polycystic kidney disease. Although polycystins are believed to interact vivo, two proteins often display dissimilar patterns gradients expression during development. In an effort understand this apparent discrepancy, we investigated how changes can affect subcellular localization polycystin-1. We show that, when is expressed alone a PKD2 null cell...
Background To understand the intrinsic cardiac developmental and functional abnormalities in pulmonary atresia with intact ventricular septum (PAIVS) free from effects secondary to anatomic defects, we performed compared single-cell transcriptomic phenotypic analyses of patient- healthy subject-derived human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) engineered tissue models. Methods Results We derived hiPSC lines 3 patients PAIVS subjects differentiated them into...
Polycystin-2 (PC2), the gene product of one two genes mutated in dominant polycystic kidney disease, is a member transient receptor potential cation channel family and can function as intracellular calcium (Ca 2+ ) release channel. We performed yeast two-hybrid screen by using NH 2 terminus PC2 identified syntaxin-5 (Stx5) putative interacting partner. Coimmunoprecipitation studies cell lines tissues confirmed interaction with Stx5 vivo. In vitro binding assays showed that between direct...