A5100656189- Lipid Membrane Structure and Behavior
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Receptor Mechanisms and Signaling
- Force Microscopy Techniques and Applications
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Retinal Development and Disorders
- Nanoparticle-Based Drug Delivery
- Photoreceptor and optogenetics research
- Protein Kinase Regulation and GTPase Signaling
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Nuclear Receptors and Signaling
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Hormonal Regulation and Hypertension
- Cardiac Structural Anomalies and Repair
- RNA Research and Splicing
- Growth Hormone and Insulin-like Growth Factors
- Genomics and Rare Diseases
- Neuroscience and Neuropharmacology Research
- Acute Myeloid Leukemia Research
Southeast University
2019-2022
Hong Kong University of Science and Technology
2002-2021
University of Hong Kong
2002-2021
Milbank Memorial Fund
2011-2012
Sun Yat-sen University
2007
Memorial Sloan Kettering Cancer Center
2006
Biotechnology Research Institute
2003
Baylor College of Medicine
2000-2002
California Institute of Technology
2000
The Brownian motion of molecules at thermal equilibrium usually has a finite correlation time and will eventually be randomized after long delay time, so that their displacement follows the Gaussian statistics. This is true even when have experienced complex environment with time. Here, we report lateral acetylcholine receptors on live muscle cell membranes does not follow statistics for normal diffusion. From careful analysis large volume protein trajectories obtained over wide range...
Liposomes are the most valuable nanocarriers in clinical use because of their biocompatibility, biodegradation, and effective encapsulation hydrophilic or hydrophobic drugs. However, applications limited by structure functions common phospholipids used as main component liposomes. In this work, novel series thioether phosphatidylcholines (S-PCs) S-PC-based liposomes (S-LPs) were developed for reactive oxygen species (ROS)-responsive drug release. First all, S-PCs with different chain lengths...
Regulators of G protein signaling (RGS) stimulate the GTPase activity Gα subunits and probably play additional roles. Some RGS proteins contain a Gγ subunit-like (GGL) domain, which mediates specific interaction with Gβ5. The role such interactions in function is unclear. can accelerate kinetics coupling protein-coupled receptors to G-protein-gated inwardly rectifying K+ (GIRK) channels. Therefore, we coupled m2-muscarinic acetylcholine GIRK channels Xenopus oocytes evaluate effect Gβ5 on...
Proteins of the regulator G protein signaling (RGS) family accelerate GTP hydrolysis by alpha subunits (G(alpha)) proteins, leading to rapid recovery cascades. Many different RGS proteins can an individual G(alpha), and rates G(alpha)s be enhanced same protein. Consequently, mechanisms for specificity in regulation residues involved remain unclear. Using evolutionary trace (ET) method, we have identified a cluster domain that includes RGS-G(alpha) binding interface extends include additional...
Nerve growth factor (NGF) is required for the development of sympathetic neurons and subsets sensory neurons. Our current knowledge on molecular mechanisms underlying biological functions NGF in part based studies with PC12 rat pheochromocytoma cells, which differentiate into neuron-like cells upon treatment. Here we report that expression leukemia inhibitory receptor (LIFR), one signaling molecules shared by several neuropoietic cytokines interleukin-6 family, specifically up-regulated...
The functional receptor complex of ciliary neurotrophic factor (CNTF), a member the gp130 family cytokines, is composed CNTF, CNTF alpha (CNTFR), gp130, and leukemia inhibitory (LIFR). However, nature receptor-mediated interactions in this has not yet been resolved. To address issue we have determined solution structure C-terminal or BC domain CNTFR studied with LIFR gp130. We reported previously that membrane distal cytokine-binding (CBD1) could interact vitro soluble (sCNTFR) absence CNTF....
Ciliary neurotrophic factor (CNTF) is a member of the gp130 family cytokines. The functional receptor complex CNTF composed α (CNTFR), and leukemia inhibitory (LIFR). Three regions on have been identified as binding sites for its receptors. ligand–receptor interactions are mediated through cytokine domains (CBDs) and/or immunoglobulin‐like However, in case CNTF, precise nature protein–protein contacts signaling has not yet resolved. In this study, we provide first demonstration that membrane...
Ciliary neurotrophic factor (CNTF) forms a functional receptor complex containing the CNTF receptor, gp130, and leukemia inhibitory (LIFR). However, nature stoichiometry of receptor‐mediated interactions in this have not yet been fully resolved. We show here that signaling by CNTF, but LIF or oncostatin M (OSM), was abolished cells overexpressing LIFR mutant with N‐terminal cytokine binding domain deleted. Our results illustrate molecular differences between active those OSM provide further...
RGS9-1 is a GTPase-accelerating protein (GAP) required for rapid recovery of the light response in vertebrate rod and cone photoreceptors. Similar to its phototransduction partners transducin (G(t)) cGMP phosphodiesterase, it peripheral disc membranes, but binds membranes much more tightly. It lacks lipid modifications found on G(t) mechanism membrane attachment unknown. We have used limited proteolysis generate fragment that readily removed from under moderate salt conditions. Immunoblots...
Directed transport of proteins and other molecules in a crowded living cell is often carried out by diffusion at short distances motor-driven cargo over long distances. Here we demonstrate, both experiments theory, that anchored inside the can generate spatially varying temporally stable potential (free-energy) landscape for intracellular or membrane mesoscale. By using micropatterned substrate, introduce periodic array integrins on basal cultured Xenopus muscle cells. This patterned imposes...
We have carried out a comparative study of the lateral motion ganglioside GM1, which is glycosphingolipid residing on outer leaflet plasma membrane, and acetylcholine receptor (AChR), well-characterized ion channel. Both lipid molecules transmembrane proteins reside membranes live Xenopus muscle cells. From thorough analysis large volume individual molecular trajectories obtained from more than 300 cells over wide range sampling rates long durations, we find that GM1s AChRs share same...
The p63 gene encodes at least 10 isoforms, which can be classified into TA and ∆N isotypes (TAp63 ∆Np63 proteins) according to their differences the N termini. TAp63γ is an important transcription factor. We previously reported that peptidyl‐prolyl isomerase (PPI) Pin1 directly binds protein identified serine 12 (S ) in transactivation domain (TAD) of required for regulation its transcriptional activity. In present study, we report stimulates pro‐apoptotic activities TAp63γ; this...