A5052644071- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Histone Deacetylase Inhibitors Research
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Redox biology and oxidative stress
- PARP inhibition in cancer therapy
- Cancer-related Molecular Pathways
- Acute Myeloid Leukemia Research
- Mosquito-borne diseases and control
- Heat shock proteins research
- Nuclear Structure and Function
Chung-Ang University
2014-2023
Korea University
2022-2023
The methylation of histone H3 lysine 79 (H3K79) is an active chromatin marker and prominent in actively transcribed regions the genome; however, demethylase H3K79 remains unknown despite intensive research. Here, we show that KDM2B, also known as FBXL10 a member Jumonji C family proteins for its H3K36 activity, di- trimethyl demethylase. We demonstrate KDM2B induces transcriptional repression HOXA7 MEIS1 via occupancy promoters demethylation H3K79. Furthermore, genome-wide analysis suggests...
Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a key epigenetic regulator of DNA methylation maintenance heterochromatin formation. The roles UHRF1 in damage repair also have been emphasized recent years. However, the regulatory mechanism remains elusive. In this study, we showed that methylated by SET7 demethylation catalyzed LSD1. addition, induced response to its phosphorylation S phase prerequisite for interaction SET7. Furthermore, catalyzes conjugation polyubiquitin...
Histone H3K9 methyltransferase (HMTase) G9a-mediated transcriptional repression is a major epigenetic silencing mechanism. UHRF1 (ubiquitin-like with PHD and ring finger domains 1) binds to hemimethylated DNA plays an essential role in the maintenance of methylation. Here, we provide evidence that transcriptionally downregulated by HMTase G9a. We found increased expression G9a along transcription factor YY1 specifically represses during TPA-mediated leukemia cell differentiation. Using ChIP...
UHRF1 is a key regulator in DNA methylation maintenance. It binds histone H3K9me2/3 and hemi-methylated recruits DNMT1 to replication forks during S phase. However, the regulatory mechanism of binding activity remains unknown. In this study, we reveal that acetylation regulated by PCAF HDAC1. We show at K490 attenuates its affinity DNA. analyze genome-wide gene-expression patterns using stable cell lines discover cells where endogenous replaced with an acetyl-mimetic (UHRF1 K490Q) mutant...
Abstract The human myelogenous leukemic cell line, K562 undergoes erythroid differentiation by exposure to hemin. Here, we uncovered NSD2 as an innate differentiation-related factor through a genome-wide CRISPR library screen and explored the regulatory role of during myeloid leukemia differentiation. We found that stability was disrupted poly-ubiquitination in differentiated cells. Proteomic analysis revealed interaction between E3 ubiquitin ligase, BRCA1, which ubiquitylates NSD on K292....
Abstract Objectives The level of histone H3 lysine 79 methylation is regulated by the cell cycle and involved in proliferation. KDM2B an H3K79 demethylase. Proliferating nuclear antigen (PCNA) a component DNA replication machinery. This study aimed at elucidating molecular link between H3K79me recognition PCNA control. Materials methods We generated KDM2B‐depleted 293T cells H3‐K79R mutant‐expressing cells. Western blots were primarily utilized to examine its effect on subsequent...
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Abstract Background Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is upregulated in colon cancer cells associated silencing tumor suppressor genes (TSGs) to promote cell proliferation. Objective To investigate epigenetic modification of UHRF1 by TIP60. Whether acetylation TIP60 can induce proliferation cells. Methods Acetylation sites was predicted ASEB (Acetylation Set Enrichment Based) method identified immunoprecipitation assay using anti-pan-acetyl lysine antibody vitro...
SET/TAF-Iβ, a subunit of the inhibitor acetyltransferases (INHAT) complex, exhibits transcriptional repression activity by inhibiting histone acetylation. We find that SET/TAF-Iβ regulates mono-ubiquitination H2A at lysine 119 (H2AK119ub), which is involved in polycomb-mediated repression, HCT116 cells. In this report, we demonstrate acts as an E2 ubiquitin-conjugating enzyme for PRC1-independent H2AK119ub. Furthermore, identify MIB1 E3 ligase partner using LC-MS/MS and vitro ubiquitination...
Abstract The methylation of histone H3 lysine 79 (H3K79) is an active chromatin marker and prominant in actively transcribed regions the genome. However, demethylase H3K79 remains unknown despite intensive research. Here, we show that KDM2B (also known as FBXL10), a member Jumonji C family proteins for its H3K36 activity, di- tri-methyl demethylase. We demonstrate induces transcriptional repression HOXA7 MEIS1 via occupancy promoters demethylation H3K79. Furthermore, genome-wide analysis...
The oncogene protein DEK is an abundant and ubiquitous nuclear with implications in acute myelogenous leukemia, as translocation which results the formation of a DEK-CAN fusion protein. In previous study, we have identified that negatively regulated peroxiredoxin 6 (Prdx 6) transcription synergistically p65 subunit NF-κB. this further investigated DEK-mediated transcriptional regulation Prdx during leukemia cell differentiation. Using Chromatin Immunoprecipitation analysis reporter assays,...
The repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) is crucial for maintaining genomic integrity and involved in numerous fundamental biological processes. Post-translational modifications proteins play an important role regulating repair. Here, we report that the methyltransferase SET7 regulates HR-mediated DSB methylating TIP60, a histone acetyltransferase tumor suppressor gene expression protein stability. We show targets TIP60 methylation at K137, which...