A5035496224- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Protein Degradation and Inhibitors
- Nuclear Structure and Function
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Redox biology and oxidative stress
- Muscle Physiology and Disorders
- Chromosomal and Genetic Variations
- Genomics, phytochemicals, and oxidative stress
- FOXO transcription factor regulation
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Glutathione Transferases and Polymorphisms
- DNA Repair Mechanisms
- Thyroid Cancer Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Circadian rhythm and melatonin
- Peptidase Inhibition and Analysis
Chung-Ang University
2016-2025
Samsung Medical Center
2017
Korea Research Institute of Bioscience and Biotechnology
2011
Asan Medical Center
2008
University of Ulsan
2008
Ulsan College
2008
University of Pennsylvania
2001-2004
Seoul National University Dental Hospital
2004
Johns Hopkins University
2004
Seoul National University
2004
Rhythmic gene expression is central to the circadian control of physiology in mammals. Transcriptional activation Per and Cry genes by heterodimeric bHLH-PAS proteins a key event feedback loop that drives rhythmicity; however, mechanism not clearly understood. Here we show transcriptional coactivators histone acetyltransferases, p300/CBP, PCAF, ACTR associate with proteins, CLOCK NPAS2, regulate positively clock expression. Furthermore, Cry2 mediated repression NPAS2:BMAL1 overcome...
Runx2 is a critical transcription factor for osteoblast differentiation. Regulation of expression levels and transcriptional activity important bone morphogenetic protein (BMP)-induced Previous studies have shown that extracellular signal-regulated kinase (Erk) activation enhances the BMP-induced acetylation increases stability activity. Because BMP signaling induces Erk in osteoblasts, we sought to investigate whether regulates stability. by overexpression constitutively active MEK1...
Posttranslational modifications of the Forkhead family transcription factor, FOXO1, have been known to important regulatory implications in its diverse activities. Several types including acetylation, phosphorylation, and ubiquitination, reported. However, lysine methylation FOXO1 has not yet identified. Here, we reported that is methylated by G9a at K273 residue vitro vivo. Methylation increased interaction between a specific E3 ligase, SKP2, decreased protein stability. In addition,...
Abstract Vascular calcification (VC) is often associated with cardiovascular and metabolic diseases. However, the molecular mechanisms linking VC to these diseases have yet be elucidated. Here we report that MDM2-induced ubiquitination of histone deacetylase 1 (HDAC1) mediates VC. Loss HDAC1 activity via either chemical inhibitor or genetic ablation enhances protein, but not mRNA, reduced in cell animal models human calcified coronary artery. Under calcification-inducing conditions,...
The methylation of histone H3 lysine 79 (H3K79) is an active chromatin marker and prominent in actively transcribed regions the genome; however, demethylase H3K79 remains unknown despite intensive research. Here, we show that KDM2B, also known as FBXL10 a member Jumonji C family proteins for its H3K36 activity, di- trimethyl demethylase. We demonstrate KDM2B induces transcriptional repression HOXA7 MEIS1 via occupancy promoters demethylation H3K79. Furthermore, genome-wide analysis suggests...
Histone acetylation by p300/CBP and PCAF coactivators is considered to be a key mechanism of chromatin modification transcriptional regulation. A multiprotein cellular complex, INHAT (inhibitor acetyltransferases), containing the Set/TAF-Iβ oncoprotein pp32 strongly inhibits HAT activity histone masking. Here we report that complex its subunits have overlapping but distinct inhibitory binding characteristics. We provide evidence suggesting can regulated physical association with other...
Histone lysine methylation and demethylation are considered critical steps in transcriptional regulation. In this report, we performed chromatin immunoprecipitation with microarray technology (ChIP-chip) analysis to examine the genome-wide occupancy of H3K9-me2 during all-trans-retinoic acid (ATRA)-induced differentiation HL-60 promyelocytic leukemia cells. Using approach, found that KDM3B, which contains a JmjC domain, was downregulated through recruitment corepressor complex. Furthermore,...
Histone lysine methylation, as one of the most important factors in transcriptional regulation, is associated with a various physiological conditions. Using bioinformatics search, we identified and subsequently cloned mouse SET domain containing 3 (SETD3) (Su(var)3-9, Enhancer-of-zeste Trithorax) Rubis-subs-bind domains. SETD3 novel histone H3K4 H3K36 methyltransferase activation activity. expressed abundantly muscular tissues and, when overexpressed, activates transcription muscle-related...
The tumor suppressor p53 responds to a wide variety of cellular stress signals. Among potential regulatory pathways, post-translational modifications such as acetylation by CBP/p300 and PCAF have been suggested for modulation activity. However, exactly how is modulated remains poorly understood. Here, we found that SET/TAF-Iβ inhibited p300- PCAF-mediated in an INHAT (inhibitor histone acetyltransferase) domain-dependent manner. interacted with repressed transcription target genes....
Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a key epigenetic regulator of DNA methylation maintenance heterochromatin formation. The roles UHRF1 in damage repair also have been emphasized recent years. However, the regulatory mechanism remains elusive. In this study, we showed that methylated by SET7 demethylation catalyzed LSD1. addition, induced response to its phosphorylation S phase prerequisite for interaction SET7. Furthermore, catalyzes conjugation polyubiquitin...
The proto‐oncogene protein DEK has been implicated in the t(6;9) chromosomal translocation associated with a subtype of acute myelogenous leukemia (AML), which results formation DEK‐CAN fusion protein. Histone acetylation is an important post‐translational modification involved transcriptional regulation. In this study, we report that acidic domain containing interacts histones and exerts potent inhibitory effect on both p300 PCAF‐mediated histone acetyltransferase activity transcription....
Abstract Severe Clonorchis sinensis infection is a significant risk factor for malignant changes in bile ducts and surrounding liver tissues occurring as result of direct contact with C. worms their excretory–secretory products (ESP). However, the intrinsic molecular mechanisms involved these processes remain obscure. To determine effects on protein expression host duct epithelium, we examined proteomic profile human cholangiocarcinoma cell line (HuCCT1) treated ESP at 24 h. Using...
Oxidative stress triggered by amyloid beta (Aβ) accumulation contributes substantially to the pathogenesis of Alzheimer's disease (AD). In present study, we examined involvement antioxidant activity peroxiredoxin 6 (Prdx 6) in protecting against Aβ25–35-induced neurotoxicity rat PC12 cells. Treatment cells with Aβ25-35 resulted a dose- and time-dependent cytotoxicity that was associated increased intracellular reactive oxygen species (ROS) mitochondria-mediated apoptotic cell death,...
Histone H3K9 methyltransferase (HMTase) G9a-mediated transcriptional repression is a major epigenetic silencing mechanism. UHRF1 (ubiquitin-like with PHD and ring finger domains 1) binds to hemimethylated DNA plays an essential role in the maintenance of methylation. Here, we provide evidence that transcriptionally downregulated by HMTase G9a. We found increased expression G9a along transcription factor YY1 specifically represses during TPA-mediated leukemia cell differentiation. Using ChIP...