A5056402283- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Microbial bioremediation and biosurfactants
- Salmonella and Campylobacter epidemiology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Natural Products and Biosynthesis
- Bioactive Compounds and Antitumor Agents
- Plant-Microbe Interactions and Immunity
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Wastewater Treatment and Nitrogen Removal
- Trace Elements in Health
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Nuclear Structure and Function
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Angiogenesis and VEGF in Cancer
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Ion Channels and Receptors
- Enzyme Catalysis and Immobilization
- Antibiotics Pharmacokinetics and Efficacy
- Vibrio bacteria research studies
Catholic University of Daegu
2014-2024
Korea Electric Power Corporation (South Korea)
2011-2024
Yonsei University
1992-2024
Korea Institute of Materials Science
2019-2020
Pusan National University
2014-2019
Kyoto University
2018
Korean National Police University
2015
Chung-Ang University
2014
Korea Institute of Ocean Science and Technology
2014
Korea Advanced Institute of Science and Technology
2014
Abstract Nuclear structure and function are governed by lamins, which intermediate filaments that mostly consist of α-helices. Different lamin assembly models have been proposed based on low resolution fragmented structures. However, their mechanisms still poorly understood at the molecular level. Here, we present crystal a long human fragment 3.2 Å allows visualization features full-length protein. The shows an anti-parallel arrangement two coiled-coil dimers, is important for process. We...
Neovessel formation is a complex process governed by the orchestrated action of multiple factors that regulate EC specification and dynamics within growing vascular tree. These have been widely exploited to develop therapies for angiogenesis-related diseases such as diabetic retinopathy tumor growth metastasis. WNT signaling has implicated in regulation development system, but detailed mechanism this remains unclear. Here, we report Dickkopf1 (DKK1) Dickkopf2 (DKK2), originally known...
Abstract Capsiate, a nonpungent capsaicin analogue, and its dihydroderivative dihydrocapsiate are the major capsaicinoids of red pepper cultivar CH-19 Sweet. In this study, we report biological actions underlying molecular mechanisms capsiate on angiogenesis vascular permeability. vitro, inhibited endothelial growth factor (VEGF)–induced proliferation, chemotactic motility, capillary-like tube formation primary cultured human cells. They also sprouting cells in rat aorta new blood vessels...
Abstract Background Breast cancer is one of the leading causes women's death worldwide. It important to discover a reliable biomarker for detection breast cancer. Plasma most ideal source discovery since many cells cross-communicate through secretion soluble proteins into blood. Methods proteomes obtained from 6 patients and normal healthy women were analyzed by using isotope-coded affinity tag (ICAT) labeling approach tandem mass spectrometry. All plasma samples used depleted highly...
The pathogenesis of type 2 diabetes mellitus (T2DM) is closely associated with mitochondrial functions in insulin-responsive tissues. proteome, compared the genome, which only contains 37 genes humans, can provide more comprehensive information for thousands proteins regarding T2DM-associated functions. However, protein signatures tissues are still unclear. Here, we performed extensive proteome profiling mitochondria from skeletal muscles nine T2DM patients and nondiabetic controls. A...
There is an emerging interest in protein expression profiling with the aim of identifying novel diagnostic markers and therapeutic targets breast cancer. We analyzed cancer tissues by 2-D DIGE using a narrow range IPG strip (pH 5.5-6.7) after immunodepletion serum albumin Ig. Sixty-three spots were detected more than ±1.8-fold differences (p <0.05 for three technical replicates) from set tissue samples which tumor nontumor randomly selected six subjects pooled separately. Of these, 53...
Approximately 97% of patients with ovarian granulosa cell tumours (GCTs) bear the C134W mutation in FOXL2; however, pathophysiological mechanism this is unknown. Here we report how affects GCT development. Sequential posttranslational modifications mutant occur where hyperphosphorylation at serine 33 (S33) by GSK3β induces MDM2-mediated ubiquitination and proteasomal degradation. In contrast, S33 wild-type FOXL2 underphosphorylated, leading to its SUMOylation stabilization. This prominent...
The balance between major DNA double-strand break (DSB) repair pathways is influenced by binding of the Ku complex, a XRCC5/6 heterodimer, to DSB ends, initiating non-homologous end joining (NHEJ) but preventing additional resection and homologous recombination (HR). However, key molecular cue for recruitment sites unknown. Here, we report that FOXL2, forkhead family transcriptional factor, directs pathway choice acetylation-dependent Ku. Upon induction, SIRT1 translocates nucleus...
An erythromycin esterase (molecular mass 51 200 Da) was purified from Pseudomonas sp. GD100, which isolated a salmon hatchery sediment sample Washington State. The pI of the protein 4.5–4.8. enzyme inhibited by 1 mM mercuric acid, and had substrate specificity for structurally related 14-membered macrolides, decreased in order oleandomycin, A enol ether. activity varied with temperature, but effect pH minimal at 6.0–9.0. half-life estimated to be 8.9 h 35°C 0.23 55°C, activation energy...
Abstract Here, we report a resistance mechanism that is induced through the modulation of 16S ribosomal RNA (rRNA) processing on exposure Escherichia coli cells to aminoglycoside antibiotics. We observed decreased expression levels RNase G associated with increased III activity rng mRNA in subgroup E. isolates transiently acquired low kanamycin or streptomycin. Analyses rRNA from aminoglycoside-resistant cells, addition mutagenesis studies, demonstrated accumulation precursors containing 3–8...
Nuclear lamins maintain the nuclear envelope structure by forming long linear filaments via two alternating molecular arrangements of coiled-coil dimers, known as A11 and A22 binding modes. The mode is characterized antiparallel interactions between coil 1b domains, whereas facilitated 2 domains lamin. junction A11- A22-interacting dimers in lamin tetramer produces another parallel head-tail interaction 1a C-terminal region 2, called ACN interaction. During mitosis, phosphorylation...
Transfer RNA halves (tRHs) have various biological functions. However, the biogenesis of specific 5′-tRHs under certain conditions remains unknown. Here, we report that inositol-requiring enzyme 1α (IRE1α) cleaves anticodon stem-loop region tRNAGly(GCC) to produce (5′-tRH-GlyGCC) with highly selective target discrimination upon endoplasmic reticulum (ER) stress. Levels 5′-tRH-GlyGCC positively affect cancer cell proliferation and modulate mRNA isoform both in vitro vivo; these effects...
ABSTRACT Erythromycin has been widely used in food‐producing animals and humans, is frequently detected as an organic pollutant U.S. streams. In batch experiments with homoionic clays, the Freundlich isotherms were determined at 10 25°C. The adsorption of erythromycin A was strongly influenced by clay type, exchanged cations, pH bulk solutions, acidity surfaces. formation clay–erythromycin complexes thermodynamically favorable except for K + – Fe 3+ –exchanged montmorillonites, since...
Summary Twenty‐seven Gram‐positive strains were characterized physiologically and numerically classified them into four groups according to their specific activities for utilization of linear alkyl ethers (AEs), cyclic AEs, monoalkoxybenzenes 1,4‐diethoxybenzene. The comparative analysis the 16S ribosomal RNA gene 16S−23S intergenic spacer region showed that they belonged genera Rhodococcus Gordonia . Alkyl ether‐utilizing rhodococci appeared involve various diverse cytochromes P450 families...