A5005827139- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Reproductive Biology and Fertility
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- FOXO transcription factor regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Effects and risks of endocrine disrupting chemicals
- Luminescence and Fluorescent Materials
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
- interferon and immune responses
- Cancer-related Molecular Pathways
- Nanoplatforms for cancer theranostics
- Ovarian cancer diagnosis and treatment
- Reproductive System and Pregnancy
- Molecular Sensors and Ion Detection
- Bacteriophages and microbial interactions
- Pregnancy-related medical research
- Vibrio bacteria research studies
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Sperm and Testicular Function
- Pregnancy and preeclampsia studies
Chung-Ang University
2015-2024
Jeonbuk National University
2015
Korea Institute of Ocean Science and Technology
2014
Catholic University of Daegu
2014
Pusan National University
2014
Korea Advanced Institute of Science and Technology
2014
CHA University
2005-2012
Stanford University
2000-2004
University of Michigan
1999-2001
MCL-1 (myeloid cell leukemia-1) is an antiapoptotic BCL-2 family protein discovered as early induction gene during myeloblastic leukemia differentiation. This survival has the homology (BH) domains 1, 2, and 3 a C-terminal transmembrane region. We identified short splicing variant of mRNA in human placenta encoding protein, termed (MCL-1S), with altered C terminus compared full-length long (MCL-1L), leading to loss BH1, BH2, domains. Analysis indicated that MCL-1S results from out exon 2...
Abstract Premature ovarian failure in a subgroup of women with blepharophimosis-ptosis-epicanthus inversus type 1 syndrome has been associated nonsense mutations the gene encoding Forkhead transcription factor, L2 (FOXL2). However, exact function FOXL2 ovary is unclear. We investigated expression mouse during follicular development and maturation by RT-PCR situ hybridization. The mRNA expressed ovaries throughout adulthood localized to undifferentiated granulosa cells small medium follicles...
Fluorescence-based amine-reactive dyes are highly valuable for the sensing of amines and labeling biomolecules. Although it would be desirable, large changes in emission spectra intensity seldom accompany conjugation known to their target molecules. On contrary, amide bond formation between pentafluorophenyl (2-PFP) succinimidyl (2-NHS) esters meso-carboxyBODIPY results significant maxima (Δλ: 70–100 nm) (up 3000-fold), enabling fast (down 5 min) selective fluorogenic detection amines, amino...
Malignant melanoma, characterized by its ability to metastasize other organs, is responsible for 90% of skin cancer mortality. To investigate alterations in the cellular metabolome and lipidome related melanoma metastasis, gas chromatography-mass spectrometry (GC-MS) direct infusion-mass (DI-MS)-based metabolic lipidomic profiling were performed on extracts normal human melanocyte (HEMn-LP), low metastatic (A375, G361), highly (A2058, SK-MEL-28) cell lines. In this study, metabolomic...
Human thyrotropin (TSH), luteotropin (LH), follitropin (FSH), and chorionic gonadotropin are members of the heterodimeric glycoprotein hormone family. The common α subunit forms noncovalent heterodimers with different β subunits. Two novel human hormonelike genes, α2 (A2) β5 (B5), recently have been identified. Using a yeast two-hybrid assay, two subunits were found as potential heterodimerization partners. Immunological analyses confirmed A2 B5 in transfected cells their colocalization...
Human thyrotropin (TSH), luteotropin (LH), follitropin (FSH), and chorionic gonadotropin are members of the heterodimeric glycoprotein hormone family. The common α subunit forms noncovalent heterodimers with different β subunits. Two novel human hormonelike genes, α2 (A2) β5 (B5), recently have been identified. Using a yeast two-hybrid assay, two subunits were found as potential heterodimerization partners. Immunological analyses confirmed A2 B5 in transfected cells their colocalization...
Mutations in FOXL2 are responsible for blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) type I, which affected women exhibit premature ovarian failure. FOXL2-null mice showed defects granulosa cell development during folliculogenesis. We screened a rat yeast two-hybrid cDNA library to identify FOXL2-interacting proteins and found steroidogenic factor-1 (SF-1). Here, we show that human SF-1 interact cells negatively regulates the transcriptional activation of enzyme, CYP17, by...
The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench dibrominated product because its self-assembly into emissive J-aggregates provide turn-on signal. Applications this fluorogen to assays, dipstick sensors, fluorescence...
Abstract Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used treatment regimens against V. infection. However, the therapeutic effects of limited by multidrug resistance. In this study, we demonstrated that an antimicrobial peptide (AMP), HPA3P His , loaded onto a gold nanoparticle-DNA aptamer (AuNP-Apt) conjugate (AuNP-Apt-HPA3P ) is effective tool infection vivo mice. induced bacterial cell death through disruption membrane integrity . The introduction...
A fluorescent probe based on an excimer-forming benzothiazolyl-cyanovinylene (CV) dye was developed to target the apoptotic protease caspase-3. Upon action of caspase-3, water-soluble Ac-DEVD-NH-CV, which is weakly green emissive in aqueous solution, converted hydrophobic CV-NH2, spontaneously aggregates. Aggregation CV-NH2 promotes excimer emission CV dye, allows for study caspase-3 activity vitro and imaging enzyme living cells because large red shift enhanced fluorescence signal probe.
MINT‐7255756: MCL1‐ES (uniprotkb:Q07820‐2) physically interacts (MI:0914) with COX IV (uniprotkb:P13073) by anti tag coimmunoprecipitation (MI:0007)
Summary During the course of experiments aimed at identifying genes with ribonuclease III (RNase III)‐dependent expression in Escherichia coli , we found that steady state levels bdm mRNA were dependent on cellular concentrations RNase III. The half‐lives adventitiously overexpressed and activities a transcriptional bdm‘–’cat fusion observed to be III, indicating existence cis ‐acting elements responsive In vitro vivo cleavage analyses identified two motifs, one 5′‐untranslated region other...
In this study, single-stranded DNA functionalized gold nanoparticles (AuNP GDS) were proved to be efficient gene delivery systems for oligo antisense DNAs specific any of interest without affecting normal cell physiology.
Use of non-biological agents for mRNA delivery into living systems in order to induce heterologous expression functional proteins may provide more advantages than the use DNA and/or biological vectors delivery. However, low efficiency live animals, using systems, has hampered as a therapeutic molecule. Here, we show that gold nanoparticle-DNA oligonucleotide (AuNP-DNA) conjugates can serve universal vehicles efficient human cells, well xenograft tumors generated mice. Injections BAX loaded...
The development of specifically targeted nanoparticles for subcellular organelles modified with a low-molecular-weight organic compound as drug nanocarriers can bring about wide applications in cancer therapy. However, their utility has been hampered by low selectivity, poor biodistribution, and limited efficiency. Herein, we report the aggregation behavior triphenylphosphonium-appended coumarin probe (TPP-C) an aqueous solution its mitochondria-targeting probe, delivery carrier, which is...
Abstract The selective monitoring of G‐quadruplex (G4) structures in living cells is important to elucidate their functions and reveal value as diagnostic or therapeutic targets. Here we report a fluorogenic probe ( CV2 ) able selectively light‐up parallel G4 DNA over antiparallel topologies. was constructed by conjugating the excimer‐forming CV dye with peptide sequence l ‐Arg‐ ‐Gly‐glutaric acid) that specifically recognizes G4s. forms self‐assembled, red excimer‐emitting nanoaggregates...
Mcl-1L (myeloid cell leukemia-1 long) is an antiapoptotic Bcl-2 family protein discovered as early induction gene during leukemia differentiation. Previously, we identified Mcl-1S (short) a short splicing variant of the Mcl-1 with proapoptotic activity. To identify Mcl-1-interacting proteins, performed yeast two-hybrid screening and found cDNAs encoding tankyrase 1. This possesses poly(ADP-ribose) polymerase activity presumably facilitates turnover substrates following ADP-ribosylation. In...