// Hye-Youn Kim 1, * , Kyung-Min Lee So-Hyun 1 Yeo-Jung Kwon Young-Jin Chun Hyung-Kyoon Choi College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic Korea These authors have contributed equally to this work Correspondence to: Chun, email: yjchun@cau.ac.kr Choi, hykychoi@cau.ac.kr Keywords: metastasis, breast cancer cells, metabolomics, lipidomics, PLSR Received: May 04, 2016 Accepted: August 11, Published: 24, ABSTRACT This study conducted comprehensive and comparative metabolic...

Malignant melanoma, characterized by its ability to metastasize other organs, is responsible for 90% of skin cancer mortality. To investigate alterations in the cellular metabolome and lipidome related melanoma metastasis, gas chromatography-mass spectrometry (GC-MS) direct infusion-mass (DI-MS)-based metabolic lipidomic profiling were performed on extracts normal human melanocyte (HEMn-LP), low metastatic (A375, G361), highly (A2058, SK-MEL-28) cell lines. In this study, metabolomic...

Colorectal cancer (CRC) is the third most common cancer, and associated with a high percentage of cancer-related death globally. Furthermore, success rate therapeutic treatment for CRC patients mainly depends on status metastasis. Therefore, novel drugs or techniques should be discovered metastatic CRC. In this study, we selected Astaxanthin (AXT), one carotenoids, as metastasis inhibitor through high-throughput drug screening based invadopodia staining, confirmed anti-migratory...

DNAJB9, a member of the heat shock protein 40 family, acts as multifunctional player involved in maintenance their client proteins and cellular homeostasis. However, mechanistic action DNAJB9 human malignancies is yet to be fully understood. In this study, we found that ectopic restoration inhibits migration, invasion, vivo metastasis, lung colonization triple-negative breast cancer (TNBC) cells. Mechanistically, stabilizes FBXO45 by suppressing self-ubiquitination reduces abundance ZEB1...

Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is a complex gastrointestinal disorder with multifactorial etiology, environmental triggers, autoimmune mechanisms, genetic predisposition. Despite advancements in therapeutic strategies for IBD, its associated mortality rate continues to rise, which often attributed unforeseen side effects of conventional treatments. In this context, we explored the potential Ecklonia cava extract (ECE) derived from...

Abstract Distant metastasis is a significant hallmark affecting to the high death rate of patients with triple-negative breast cancer (TNBC). Thus, it crucial identify and develop new therapeutic strategies hinder metastasis. While emerging studies have hinted pivotal role glucose-regulated protein 94 (GRP94) in tumorigenesis, exact biological functions molecular mechanisms GRP94 modulating remain be elucidated. Our study demonstrated an increased expression TNBC correlated metastatic...

Panax ginseng C.A. Meyer is a herb used for medicinal purposes, and its discrimination according to cultivation age has been an important practical issue. This study employed Fourier-transform infrared (FT-IR) spectroscopy with multivariate statistical analysis obtain prediction model discriminating ages (5 6 years) three different parts (rhizome, tap root, lateral root) of P. ginseng. The optimal partial-least-squares regression (PLSR) models samples were determined by selecting...

Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn’s disease, is a complex gastrointestinal disorder with multifactorial etiology, environmental triggers, autoimmune mechanisms, genetic predisposition. Despite advancements in therapeutic strategies for IBD, its associated mortality rate continues to rise, which often attributed unforeseen side effects of conventional treatments. In this context, we explored the potential Ecklonia cava extract (ECE), derived from an...

BP10A is a novel two-herb medicine formula, consisting of Descurainiae sophia Semen and Peucedani praeruptorum Radix. This study was done to evaluate the antitumor efficacy its effect on anticancer drugs oxaliplatin irinotecan (CPT-11) in colon tumor xenograft model. Chemical constituents from ethanol extracts were characterized with ultra-performance liquid chromatography (UPLC) each constituent quantified UPLC-diode array detector method. Our showed that exerted cytotoxic effects two...

Recent evidence suggests that patient derived xenograft (PDX) models can maintain certain pathological and molecular features of the original disease. However, these characterizations are limited to immunohistochemistry or by tissue microarray analysis. We conducted a high-throughput sequencing primary colon tumor PDX has not been reported yet. Fresh cancer tissues originate from surgery were implanted into subcutaneous space 6- 8-week-old female BALB/c nu/nu NOD/SCID mice serially passaged...

KIOM-CRC#BP3B (BP3B) is a novel herbal prescription that composed of three plant extracts. Our preliminary study identified BP3B exhibited potent anti-proliferative activity against various types cancer cell lines in vitro. Because the vivo anti-tumor effect not evaluated before clinical trial, we want to test it using patient's samples.To confirm anti-cancer BP3B, used genetically characterized patient-derived colon tumor xenograft (PDTX) mouse model. Anti-cancer was with apoptosis,...

SMA- and MAD-related protein 7 (SMAD7) is a general inhibitor of transforming growth factor-β (TGF-β) signaling that acts through interaction degradation TGF-β receptors. SMAD7 has been demonstrated to be transcriptionally upregulated in chemical-induced skin tumors TGF-β-treated normal keratinocytes. To evaluate the function carcinogenesis vivo, Smad7 transgenic mice specifically express either wild-type (WT) (TG-Smad7-WT) or mutant (TG-Smad7-MT) keratinocytes, as well keratinocyte-specific...

Epidermal growth factor (EGF) regulates various cellular events, including proliferation, differentiation, migration, and tumorigenesis. For the maintenance of homeostasis, EGF signaling should be tightly regulated to prevent aberrant activation. Smad7 has been known as inhibitory Smad that blocks signal transduction transforming β. In process cell proliferation or transformation, shown opposite activities a promoter suppressor depending on types microenvironments. We found overexpression in...

T cell protein tyrosine phosphatase N2 (PTPN2) is a phosphotyrosine-specific nonreceptor and ubiquitously expressed in tissues. Although PTPN2 functions as an important regulator different signaling pathways, it still unclear what specific target of how regulated lipopolysaccharide (LPS)-induced inflammatory pathway. Here, we found that deficiency downregulated the expression LPS-mediated pro-inflammtory cytokine genes. Conversely, overexpression Raw264.7 cells enhanced secretion those...

본 연구에서는 기장 해안의 해수에서 분리한 해양 미생물로부터 해조류의 난소화성 복합다당류를 분해하는 균주를 분리하고 동정하여 Microbulbifer sp. SC092로 명명하였다. SC092 미생물에 의하여 생성된 조효소의 기질 특이성 분석으로 agar 및 alginic acid를 효소활성이 각각 146.2%로 agarase와 alginase 높은 것으로 나타났다. 감태 효소분해산물의 항산화력은 DPPH radical 소거능과 SOD 활성으로 측정하였으며, 활성은 84.1, 89.6%로 혈당 강하능은 <TEX>${\alpha}$</TEX>-glucosidase 활성억제 효과를 측정하였으며 58.7%로 항염증 소재로써 이용 가능성은 인체의 위의 pH와 비슷한 조건에서 아질산염소거능 측정으로 확인하였는데, 0.5 mg/ml 농도에서 56.3%를 나타내어 positive control로 사용한 Vit C 보다 활성을 보였다. 알코올분해능은 ADH와 ALDH 측정한 결과는...

<title>Abstract</title> Distant metastasis is a significant hallmark affecting to the high death rate of patients with triple-negative breast cancer (TNBC). Thus, it crucial identify and develop new therapeutic strategies hinder metastasis. While emerging studies have hinted pivotal role glucose-regulated protein 94 (GRP94) in tumorigenesis, exact biological functions molecular mechanisms GRP94 modulating remain be elucidated. Our study demonstrated an increased expression TNBC correlated...