Stroke is one of the leading causes adult disability and death in developing countries. However, early diagnosis difficult no reliable biomarker currently available. Thus, we applied a 1H-NMR metabolomics approach to investigate altered metabolic pattern plasma urine from patients with cerebral infarctions sought identify biomarkers associated stroke.Metabolic profiles infarctions, especially small vessel occlusion, were investigated using spectroscopy coupled multivariate statistical...

Loss of skeletal muscle mass and function has detrimental effects on quality life, morbidity, mortality, is particularly relevant in aging societies. The enhancement mitochondrial shown promise promoting differentiation function. Ginsenoside Rc (gRc), a major component ginseng, various pharmacological activities; however, its effect loss remains poorly explored. In this study, we examined the gRc hydrogen peroxide (H2O2)-induced reduction cell viability C2C12 myoblasts myotubes H2O2-induced...

Bangpungtongsung-san (BTS) is a traditional Korean medicine consisting of 18 herbs, some which have antidepressant effects. Here, we used an animal model reserpine-induced depression and lipopolysaccharide (LPS)-stimulated BV2 microglia to assess the anti-neuroinflammatory effects BTS. Aside from control group, C57BL/6 mice were administered reserpine (0.5 mg/kg) daily for 10 days via intraperitoneal injection. BTS (100, 300, or 500 mg/kg), vehicle (PBS), fluoxetine (FXT, 20 was orally 1 h...

We characterized the therapeutic biological modes of action several terpenes in Poria cocos F.A Wolf (PC) and proposed a broad mode for PC. Molecular docking drug-induced transcriptome analysis were performed to confirm pharmacological mechanism PC terpene, new method, namely diffusion network analysis, was verify against Alzheimer’s disease. confirmed that compound exists only has unique through statistical-based analysis. Also, transcriptomic results could reflect clinical practice when...

Paeoniae Radix (PR) has a great therapeutic value in many clinical applications; however, the presence of various bioactive compounds and its complicated effects on human health makes precise mechanisms action unclear. This study investigated PR at molecular pathway level by profiling genome-wide gene expression changes following dose-dependent treatment lung cancer cells (A549) with water extract (WPR), ethanol extracts (EPR), as well their individual components. We found that exerts...

Although chronic treatment with glucocorticoids, such as dexamethasone, is frequently associated muscle atrophy, effective and safe therapeutics for treating atrophy remain elusive. Jakyak-gamcho-tang (JGT), a decoction of Paeoniae Radix Glycyrrhizae et Rhizoma, has long been used to relieve tension control cramp-related pain. However, the effects JGT on glucocorticoid-induced are yet be comprehensively clarified. The objective current study was validate protective effect in...

Forsythia viridissima fruit, one of Forsythiae Fructus (FF) is widely used in traditional medicine to treat diverse diseases-related clinical symptoms, including fever, pain, vomiting, nausea, and abscess. However, the safety FF has not been fully assessed. In this study, we evaluated acute oral toxicity genotoxic potential an aqueous extract fruits (EFVF). For test, male female SD rats (n = 5) orally received a single dose 5000 mg/kg EFVF. The EFVF was with battery tests, vitro bacterial...

Abstract Background Gleditsia sinensis thorns have been widely used in traditional Korean medicine for the treatment of several diseases, including obesity, thrombosis, and tumor-related diseases. The aim study is to determine antiangiogenic effect vitro vivo a bid evaluate its potential as an anticancer drug. Methods Ethanol extract (EEGS) were prepared assays. In EEGS was determined HUVEC primary cells by cell migration tube formation measuring vessel vascular endothelial migrating into...

Angiogenesis, the process of new vessel formation from pre-existing blood vasculature, is critical for continuous tumor growth and considered to be a validated antitumor target. The results our previous study demonstrate anti-angiogenic potential an extract Gleditsia sinensis thorns, which has been traditionally used in Korean medicine remedy diverse diseases, including tumors. In present study, we attempted identify active constituents ethanol G. thorns (EEGS). By virtue vitro...

The dried fruits of Forsythia viridissima have been prescribed to relive fever, pain, vomiting, and nausea in traditional medicine. Oxaliplatin (LOHP) is used treat advanced colorectal cancer; however, it frequently induces peripheral neuropathies. This study was done evaluate the neuroprotective effects an aqueous extract (EFVF) its major constituents. Chemical constituents from EFVF were characterized quantified with UHPLC-diode array detector method, three identified as arctiin,...

Radix of Asiasarum heterotropoides var. mandshuricum F. Maekawa (A. radix) has been prescribed for treating pain, allergies and inflammatory disorders in traditional oriental medicine. However, only limited information on the anticancer effects A. radix is currently available. The aim this study was to determine effect ethanol extract (EEAR) HCT-116 human colon cancer cells investigate its underlying mechanisms action. EEAR significantly induced G2/M cell cycle arrest apoptosis cells....

BP10A is a novel two-herb medicine formula, consisting of Descurainiae sophia Semen and Peucedani praeruptorum Radix. This study was done to evaluate the antitumor efficacy its effect on anticancer drugs oxaliplatin irinotecan (CPT-11) in colon tumor xenograft model. Chemical constituents from ethanol extracts were characterized with ultra-performance liquid chromatography (UPLC) each constituent quantified UPLC-diode array detector method. Our showed that exerted cytotoxic effects two...

Pharmacogenomic analysis based on drug transcriptomic signatures is widely used to identify mechanisms of action and pharmacological indications. Despite accumulating reports the efficacy medicinal herbs, related transcriptome-level analyses are lacking. The aim present study was elucidate underlying molecular Bupleuri Radix (BR), a herbal medicine, through systematic analysis. We analyzed drug-responsive transcriptome profiling A549 lung cancer cell line after treating them with multiple...

Activity-guided fractionation of an 80% EtOH extract from the aerial parts Saururus chinensis led to isolation three anti-proliferative neolignans (1–3) along with four flavonoids (4–7) and aristolactams (8–11). Their chemical structures were identified by analysis spectroscopic data. All compounds 1–11 evaluated for their activities against 28 human cancer cell lines using in vitro proliferation assay. Compounds 1–3 showed potent cervical (C33a, IC50=0.01 µM 1; 0.28 2; 2.80 3) lung...

Although the pharmacological activities of seed extract Descurainia sophia have been proven to be useful against cough, asthma, and edema, biologically active components, particularly at molecular level, remain elusive. Therefore, we aimed identify component an ethanol D. seeds (EEDS) by applying a systematic genomic approach. After treatment with EEDS, dose-dependently expressed genes in A549 cells were used query Connectivity map determine which small molecules could closely mimic EEDS...

Angiogenesis is the process of new vessel formation from pre-existing blood vasculature and critical for continuous tumor growth. We previously reported that an ethanolic extract Gleditsia sinensis thorns (EEGS) its active constituent, cytochalasin H, have anti-angiogenic activity in vitro vivo via suppression endothelial cell functions. In present study, EEGS H were observed to efficiently inhibit growth ovo xenograft model without significant toxicity. repeatedly anti-tumor anti-metastatic...

Oxaliplatin can induce peripheral neuropathy (OXIPN) as an adverse side effect in cancer patients. Until now, no effective preventive or therapeutic drug has been developed; therefore, the dose-limiting factor of OXIPN is still obstacle use oxaliplatin to treat In present study, we report for first time that aqueous extract Lithospermi radix (WLR) attenuate both vitro and vivo neuropathic models. The protective WLR on was evaluated by quantifying nerve growth (NGF)-stimulated neurite...

There is an urgent need to find antidepressants that can be administered for long periods without inducing severe side effects replace conventional control monoamine levels, such as tricyclic (TCAs), oxidase inhibitors (MAOIs), and selective serotonin reuptake (SSRI). We sought determine the antidepressant of Fraxinus rhynchophylla Hance ( F. Hance, FX) its components on a reserpine-induced mouse model. One hour after oral administration FX (30, 50, 100 mg/kg), esculin (50 esculetin fraxin...

A new tetrahydrofuran lignan, (7S,8R,7'S,8'S)-3-methoxy-3',4'-methylenedioxy-7,9'-epoxylignane-4,7',9-triol (1), and 21 known compounds 2–22 were isolated from the roots of Asiasarum heterotropoides by chromatographic separation methods. The structures all 1–22 elucidated spectroscopic analysis including 1D- 2D-NMR. Fourteen these (1–3, 7, 10, 12–17, 19, 21, 22) this species in study for first time. All isolates evaluated their anticancer activities using vitro assays. Among 22 tested...

Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, edema. Our previous results showed that ethanol extract the seeds D. (EEDS) a potent cytotoxic effect on human cancer cells. In this study, we reveal molecular events are induced by EEDS A549 lung The dose-dependent gene expression was measured via microarray analysis. Gene ontology pathway analyses were performed to identify functional involvement...

Angiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during development metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In the current study, we investigated potential of an ethanol extract Annona atemoya seeds (EEAA) in vitro vivo.The EEAA was evaluated using various vitro/in vivo models, including cell proliferation, migration, tube...