Cellular internalization of bacteriophage by surface-displayed cell penetrating peptides has been reported, though the underlying mechanism remains elusive. Here we describe in detail and intracellular trafficking stability filamentous M13 phages, cellular entry which is mediated cell-penetrating light chain variable domain 3D8 VL transbody (3D8 VL-M13) or TAT peptide (TAT-M13). Recombinant VL-M13 TAT-M13 phages were efficiently internalized into living mammalian cells via physiologically...

Eupatorium fortunei has long been used to treat nausea and poor appetite prescribed as a diuretic detoxifying drug in Chinese medicine. Recent studies have demonstrated that E. possesses anti-bacterial, anti-oxidant anti-diabetic activities, well cytotoxicity human leukemia cells. However, at non-toxic concentrations, the effects of an aqueous extract (WEF) on metastatic angiogenic potential malignant tumor cells not reported. In this study, we found WEF suppressed properties, including...

Abstract Skeletal muscle atrophy is a critical feature of cancer-induced cachexia, caused by pro-cachectic factors secreted host cells and tumor cells. Therefore, blockade these has considered reasonable target for pharmacological nutritional interventions to prevent skeletal loss under cachexia. Citrus unshiu peel (CUP) been used treating the common cold, dyspepsia, bronchial discomfort reported have activities against inflammation, allergy, diabetes, viral infection. In present study, we...

Loss of skeletal muscle mass and function has detrimental effects on quality life, morbidity, mortality, is particularly relevant in aging societies. The enhancement mitochondrial shown promise promoting differentiation function. Ginsenoside Rc (gRc), a major component ginseng, various pharmacological activities; however, its effect loss remains poorly explored. In this study, we examined the gRc hydrogen peroxide (H2O2)-induced reduction cell viability C2C12 myoblasts myotubes H2O2-induced...

Abstract Matrix Metalloproteinases (MMPs) are crucial enzymes for ultraviolet irradiation‐induced photoaging in human skin. Ultraviolet B (UVB) stimulates dermal fibroblasts to increase MMP‐1 and ‐3 expression extracellular matrix (ECM) degradation photoaging. We investigated whether phosphatase tensin homolog (PTEN)/Akt pathway is involved secretions of fibroblasts. The secretion occurred along with the PTEN Akt phosphorylation by UVB irradiation a dose‐ time‐dependent manner. However,...

Stimulation of the proapoptotic tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptors, death receptors 4 (DR4) and 5 (DR5), conventionally induces caspase-dependent apoptosis in cells. Here we report that stimulation DR4 and/or DR5 by agonistic protein KD548-Fc, an Fc-fused DR4/DR5 dual-specific Kringle domain variant, activates plasma membrane-associated Nox1 NADPH oxidase to generate superoxide anion subsequently accumulates intracellular reactive oxygen species...

Samsoeum (SSE), a traditional herbal formula, has been widely used to treat cough, fever, congestion, and emesis for centuries. Recent studies have demonstrated that SSE retains potent pharmacological efficiency in anti-allergic anti-inflammatory reactions. However, the anti-cancer activity of its underlying mechanisms not studied. Thus, present study was designed determine effect on cell death elucidate detailed mechanism.Following treatment, growth were measured using an MTT assay trypan...

Paeoniae Radix (PR) has a great therapeutic value in many clinical applications; however, the presence of various bioactive compounds and its complicated effects on human health makes precise mechanisms action unclear. This study investigated PR at molecular pathway level by profiling genome-wide gene expression changes following dose-dependent treatment lung cancer cells (A549) with water extract (WPR), ethanol extracts (EPR), as well their individual components. We found that exerts...

Although chronic treatment with glucocorticoids, such as dexamethasone, is frequently associated muscle atrophy, effective and safe therapeutics for treating atrophy remain elusive. Jakyak-gamcho-tang (JGT), a decoction of Paeoniae Radix Glycyrrhizae et Rhizoma, has long been used to relieve tension control cramp-related pain. However, the effects JGT on glucocorticoid-induced are yet be comprehensively clarified. The objective current study was validate protective effect in...

Here, we report the development of target-specific binding proteins based on kringle domain (KD) ( approximately 80 residues), a ubiquitous modular structural unit occurring across eukaryotic species. By exploiting highly conserved backbone folding by core residues, but using extensive sequence variations in seven loop regions naturally human KDs, generated synthetic KD library yeast cell surface randomizing 45 residues loops template. We isolated variants that specifically bind to...

KIOM-C was recently demonstrated to have anti-metastatic activity in highly malignant cancer cells via suppression of NF-κB-mediated MMP-9 activity. In addition, it reported be effective for clearance the influenza virus by increasing production anti-viral cytokines, such as TNF-α and IFN-γ, efficacious treatment pigs suffering from porcine circovirus-associated disease (PCVAD). this study, we investigated whether induces cell death elucidated underlying anti-cancer mechanisms. examined oral...

Rhaponticin (RA; 3'5-dihydroxy-4'-methoxystilbene 3-O-β-D‑glucopyranoside) is a component isolated from various medicinal herbs including Rheum undulatum L. RA has been reported to be an effective treatment for allergy, diabetes, thrombosis, liver steatosis, lung fibrosis and colitis. In addition, effectively inhibits tumor growth induces apoptosis; however, the effects of RA, at non-cytotoxic doses, on metastasis angiogenesis malignant cancer cells have, best our knowledge, not identified....

Bamboo shavings (Bambusae Caulis in Taeniam, BCT) are widely used as a traditional Chinese medicine to control hypertension and cardiovascular disease, alleviate fever, vomiting, diarrhea. It has been demonstrated that BCT reduces ovalbumin-induced airway inflammation by regulating pro-inflammatory cytokines, decreases tumor growth tumor-bearing mice. However, the effects of on metastatic potential malignant cancer cells detailed mechanism its anti-metastatic activity have not examined...

Abstract Background Ssanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role SHT on melanin synthesis has not been checked previously. Thus, present study was designed to determine effect α-melanocyte stimulating hormone (α-MSH)-induced melanogensis its mechanisms action murine B16F10 melanoma cells. Method Cellular content tyrosinase activity cells were determined...

Abstract Lophatheri Herba (LH), dried leaf of Lophatherum gracile Brongn, has long been used to reduce thirst and treat fever inflammation in Chinese medicine. Recent studies have shown that LH anti-viral, anti-bacterial, anti-cancer, anti-oxidant, diuretic, hyperglycemic properties. However, the effects an ethanol extract L. herba (ELH), at non-cytotoxic doses, on metastatic angiogenic abilities malignant tumor cells not reported. We found ELH significantly suppressed p38, JNK, NF-κB...

Melanoma is a highly metastatic cancer resistant to current chemotherapeutic and radiotherapeutic approaches. Several studies have shown that interactions between cells the extracellular matrix (ECM) are critical for survival invasion of cells. In this study, we examine effects methylselenol generated from selenomethionine (SeMet) by methioninase (METase) on cell proliferation, adhesion, expression integrins in murine melanoma B16F10 cells, which lungs syngeneic C57BL/6J mice. Combined...

Several components isolated from Glycyrrhizae radix rhizome (GR), including glycyrrhizin, liquiritin, and liquiritigenin, have been shown to induce cancer cell death inhibit metastasis. Isoliquiritin apioside (ISLA), a component GR, has effective for treating tetanic contraction genotoxicity. However, the effects of ISLA on metastasis angiogenesis malignant cells endothelial (ECs) not reported. In this study, we found that up 100 μM did affect proliferation but efficiently suppressed...

Jaeumganghwa-tang (JGT, Zi-yin-jiang-huo-tang in Chinese and Jiin-koka-to Japanese) is an oriental herbal formula that has long been used as a traditional medicine to treat respiratory kidney diseases. Recent studies revealed JGT exhibited potent inhibitory effects on allergies, inflammation, pain, convulsions, prostate hyperplasia. Several constituent herbs induce apoptotic cancer cell death. However, the anti-cancer activity of not examined. In this study, we investigated using highly...

So-Cheong-Ryong-Tang (CY), a traditional herbal formula, mainly has been shown to possess allergic rhinitis and asthma for hundreds of years in Asian countries. Although this medicine attracted scientists with investigating mechanisms action against inflammatory-related diseases, there is little available information on the anti-cancer effect CY, especially fermented form (FCY). In study, we explored chemopreventive/chemotherapeutic efficacy FCY cancer cells proved through performing vivo...

Gardeniae Fructus (GF, Zhi Zi in China), a fruit of Gardenia jasminoides Ellis, has been used traditional medicine to reduce inflammation and headache treat hepatic disorders, hypertension, icterus. In recent studies, extract raw or stir-baked GF was shown have pharmacological activities for viral infection, thrombosis, hyperlipidemia, convulsion, inflammation, oxidative stress, others. addition, baked suppressed the proteolytic altered cellular morphology tumor cells. However, effects...