We investigated the alterations of major fatty acid components in epidermis by natural aging and photoaging processes, acute ultraviolet (UV) irradiation human skin. Interestingly, we found that 11,14,17-eicosatrienoic (ETA), which is one omega-3 polyunsaturated acids, was significantly increased photoaged vivo also acutely UV-irradiated skin vivo, while it decreased intrinsically aged epidermis. The ETA content associated with enhanced expression elongase 1 calcium-independent...

Abstract Matrix Metalloproteinases (MMPs) are crucial enzymes for ultraviolet irradiation‐induced photoaging in human skin. Ultraviolet B (UVB) stimulates dermal fibroblasts to increase MMP‐1 and ‐3 expression extracellular matrix (ECM) degradation photoaging. We investigated whether phosphatase tensin homolog (PTEN)/Akt pathway is involved secretions of fibroblasts. The secretion occurred along with the PTEN Akt phosphorylation by UVB irradiation a dose‐ time‐dependent manner. However,...

Despite similar biochemical composition of human hair between races, physico-morphological characteristics are not identical in different ethnic groups. Eyelashes have been investigated far less than scalp hair, and the information available is insufficient.To obtain basic about eyelashes to clarify differences female Asians Caucasians.Twenty Asian 10 white Caucasian volunteers aged 20 29 years were admitted study. Lateral digital photographs upper taken curl-up lift-up angles measured from...

The Fitzpatrick skin classification has been a useful method to categorize cutaneous sensitivity ultraviolet radiation (UVR), although it was based originally on responses in white skin. Because the relevance of this phototype brown is question, we investigated phototypes university students by self‐reporting questionnaire and measured their MEDs Korean, After studying our explanation definition types, 707 Korean answered questionnaire. We then UVB 156 randomly selected male students. order...

Skullcapflavone II (SFII), a flavonoid derived from Scutellaria baicalensis, has been reported to have anti-inflammatory properties. However, its therapeutic potential for skin inflammatory diseases and mechanism are unknown. Therefore, this study aimed investigate the effect of SFII on TNF-α/IFN-γ-induced atopic dermatitis (AD)-associated cytokines, such as thymus- activation-regulated chemokine (TARC) macrophage-derived (MDC). Co-stimulation with TNF-α/IFN-γ in HaCaT cells is...

Abstract Mitochondria are essential organelles in cellular energy metabolism and other functions. Mitochondrial dysfunction is closely linked to damage can potentially contribute the aging process. The purpose of this study was investigate subcellular structure mitochondria their activities various environments using super-resolution stimulated emission depletion (STED) nanoscopy. We examined morphological dispersion below diffraction limit sub-cultured human primary skin fibroblasts mouse...

Melanoma is a highly metastatic cancer resistant to current chemotherapeutic and radiotherapeutic approaches. Several studies have shown that interactions between cells the extracellular matrix (ECM) are critical for survival invasion of cells. In this study, we examine effects methylselenol generated from selenomethionine (SeMet) by methioninase (METase) on cell proliferation, adhesion, expression integrins in murine melanoma B16F10 cells, which lungs syngeneic C57BL/6J mice. Combined...

Transcriptional Regulation of Proteoglycans and Glycosaminoglycan Chain-synthesizing Glycosyltransferases by UV Irradiation in Cultured Human Dermal FibroblastsVarious kinds glycosaminoglycans (GAGs) proteoglycans (PGs) have been known to be involved structural space-filling functions, as well many physiological regulations skin.To investigate ultraviolet (UV) radiation-mediated regulation GAGs PGs cultured human dermal fibroblasts, transcriptional changes types GAG chain-synthesizing...

Glycosaminoglycans are important structural components in the skin and exist as various proteoglycan forms, except hyaluronic acid.Heparan sulfate (HS), one of glycosaminoglycans, is composed repeated disaccharide units, which glucuronic acids linked to an N-acetyl-glucosamine or its sulfated forms.To investigate acute ultraviolet (UV)-induced changes HS proteoglycans (HSPGs), levels several HSPGs male human buttock were examined by immunohistochemistry real-time quantitative polymerase...

The presence of the blood group H2 antigen on membrane red cells determines type O in individuals and this serves as a precursor to A B antigens expressed types B, respectively. However, specific involvement ABH skin diseases is unknown. Therefore, we aim investigate expression tissue patients with atopic dermatitis (AD) MC903-induced AD-like mice. We demonstrated that primarily located granular horny layers healthy control individuals. AD, was absent or diminished these layers, while...

Abstract The effects of ultraviolet (UV) radiation on brain function have previously been investigated; however, the specific neurotransmitter-mediated mechanisms responsible for UV radiation-induced neurobehavioral changes remain elusive. In this study, we aimed to explore underlying changes. a mouse model, observed that irradiation skin induces deficits in hippocampal memory, synaptic plasticity, and adult neurogenesis, as well increased dopamine levels skin, adrenal glands, brain. Chronic...

Cholesterol is one of major components cell membrane and plays a role in vesicular trafficking cellular signaling. We investigated the effects cholesterol on matrix metalloproteinase-2 (MMP-2) activation human dermal fibroblasts. found that tissue inhibitor (TIMP-2) expression active form MMP-2 (64 kD) were dose-dependently increased by methyl-β-cyclodextrin (MβCD), depletion agent. In contrast, depletion-induced TIMP-2 suppressed repletion. Then we regulatory mechanism depletion....

Abstract Epidermal growth factor receptor inhibitors (EGFRIs) are widely used for treating various cancers, including lung, colon, head and neck cancers. However, EGFRIs have unique dermatological side effects, acneiform eruption, dry skin, paronychia pruritus. In this study, we investigated the molecular changes induced by an EGFRI, gefitinib, in expression of lipogenic enzymes hyaluronic acid (HA) regulatory proteins HaCaT keratinocytes, whether EGF restored these changes. cells were...

A new junction termination method employing shallow trenches filled with oxide, which successfully decreased the area, is proposed and fabricated without any complicated process such as Si-deep etching. Shallow between floating field limiting rings redistributed single electric peak into two peaks so that breakdown voltage could be increased same area. The experimental results show area by more than 25% compared to a conventional ring structure when voltages are equal.

Abstract ABO blood group is determined by carbohydrate antigens, called ABH antigens. It has been known that the change of antigen expression, including correlation with tumor metastasis and survival; however, exact mechanism remains to be elucidated. antigens are expressed not only in cells but also several tissues. In epidermis, uppermost spinous granular layer. We investigated role on cell migration HaCaT keratinocytes, which express B antigen. Knock‐down expression small interference RNA...

Abstract Ultraviolet ( UV ) irradiation on skin triggers photoageing‐related phenotypes such as formation of wrinkles. ray upregulates matrix metalloproteinase‐1 MMP ‐1), which in turn degrades extracellular proteins, mostly collagens. Serum amyloid A1 SAA 1) is an acute‐phase protein plasma concentration increases response to inflammation. Although the expression 1 was reported, its function yet be studied. In this research, we found that increased acute ‐irradiated buttock and photoaged...

Brain ageing, the primary risk factor for cognitive impairment, occurs because of accumulation age-related neuropathologies. Identifying effective nutrients that increase function may help maintain brain health. Tomatoes and lemons have various bioactive functions exert protective effects against oxidative stress, ageing cancer. Moreover, they been shown to enhance function. In present study, we aimed investigate tomato lemon ethanolic extracts (TEE LEE, respectively) their possible...

To investigate the effect of fucosyltransferase (FUT) 1-mediated fucosylation on meibomian glands (MG), we first confirmed that FUT1 and its fucosylated products were expressed in eyelid, conjunctiva skin wild-type (WT) mice, whereas their mRNA protein levels downregulated Fut1 knock-out (KO) mice. We then evaluated age-dependent changes total acinar areas MG, meibocyte differentiation, lipid synthesis, eyelid inflammation oxidative stress KO WT Results show both MG smaller mice than all age...

Numerous researches have focused on discovering available inhibitors of melanogenesis from natural medicinal plants with stable efficacy and safety to resolve cutaneous hyperpigmentary problems. Melochia corchorifolia Linn. (MC) has been used as folk medicine treat various diseases. However, the effect MC remains unknown.In this study, we investigated extract its underlying mechanisms in B16F10 mouse melanoma cells.B16F10 cells were treated extract, then, cell viability, melanin content,...