A5015898373- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Protein purification and stability
- T-cell and B-cell Immunology
- Energy and Environmental Systems
- Indoor Air Quality and Microbial Exposure
- RNA and protein synthesis mechanisms
- Air Quality and Health Impacts
- Bacteriophages and microbial interactions
- interferon and immune responses
- Radioactivity and Radon Measurements
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Toxic Organic Pollutants Impact
- Galectins and Cancer Biology
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Education, Safety, and Science Studies
- Legionella and Acanthamoeba research
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Plant Virus Research Studies
- Plant tissue culture and regeneration
Ajou University
2015-2024
Daegu University
2024
Korea Institute of Industrial Technology
2024
Yuhan University
2009-2023
National Institute of Environmental Research
2001-2022
Ministry of Environment
2021
Eulji University
2020
University of Dubrovnik
2013
Chonnam National University
2009
Czech Academy of Sciences, Institute of Microbiology
2007
AbstractAutophagy is one of the survival processes cancer cells, especially in stressful conditions such as starvation, hypoxia and chemotherapeutic agents. However, its roles tumor have not yet been fully elucidated. Here, we found for first time that JAK2/STAT3 was activated HeLa cells when they were starved or treated with rapamycin. STAT3 activation associated autophagic processes, because it completely inhibited by 3-methyladenine, partially knockdown molecules blocked antioxidants,...
The NLRP3 inflammasome plays a key role in responding to pathogens, and endogenous damage mitochondria are intensively involved activation. forms multiprotein complexes its sequential assembly is important for Here, we show that ubiquitinated by the mitochondria-associated E3 ligase, MARCH5. Myeloid cell-specific March5 conditional knockout (March5 cKO) mice failed secrete IL-1β IL-18 exhibited an attenuated mortality rate upon LPS or Pseudomonas aeruginosa challenge. Macrophages derived...
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptotic cell death in a variety of tumor cells without significant cytotoxicity on normal cells. However, many cancer with defects are resistant to treatment TRAIL alone, limiting its potential as an anticancer therapeutic. Here, we report the tumoricidal activity human single-chain fragment variable, HW1, which specifically binds receptor 2 (TR2) competing for binding. HW1 single agent autophagic both...
Cellular internalization of bacteriophage by surface-displayed cell penetrating peptides has been reported, though the underlying mechanism remains elusive. Here we describe in detail and intracellular trafficking stability filamentous M13 phages, cellular entry which is mediated cell-penetrating light chain variable domain 3D8 VL transbody (3D8 VL-M13) or TAT peptide (TAT-M13). Recombinant VL-M13 TAT-M13 phages were efficiently internalized into living mammalian cells via physiologically...
Abstract A subset of monoclonal anti-DNA autoantibodies enters a variety living cells. Here, we aimed to identify the endocytic receptors recognized by an internalizing anti-nucleic acid autoantibody, 3D8 single-chain variable fragment (scFv). We found that cell surface binding and internalization scFv were inhibited markedly in soluble heparan sulfate (HS)/chondroitin (CS)-deficient or -removed cells presence HS CS. colocalized intracellularly with either proteoglycans (HSPGs) CSPGs HeLa...
Here, we report the development of target-specific binding proteins based on kringle domain (KD) ( approximately 80 residues), a ubiquitous modular structural unit occurring across eukaryotic species. By exploiting highly conserved backbone folding by core residues, but using extensive sequence variations in seven loop regions naturally human KDs, generated synthetic KD library yeast cell surface randomizing 45 residues loops template. We isolated variants that specifically bind to...
Accumulation of PLK1 at kinetochores is essential for chromosome alignment and segregation; however, the mechanism underlying recruitment to remains unresolved. The chromatin remodeller RSF1 tightly associates with centromere proteins, but its mitotic function unknown. Here we show that localizes directly binds PLK1. depletion disrupts localization kinetochores; C-terminal fragment RSF1, which can bind PLK1, sufficient restore localization. Moreover, CDK1 phosphorylates Ser1375, this...
ABSTRACT Naegleria fowleri , a free-living amoeba, exists as virulent pathogen which causes fatal primary amoebic meningoencephalitis in experimental animals and humans. Using infected immune mouse sera, we previously cloned an nfa1 gene from cDNA library of N. by immunoscreening. The (360 bp) produced recombinant 13.1-kDa protein, the Nfa1 protein showed pseudopodium-specific immunolocalization on trophozoite . In this study, role cell contact mechanism cocultured with target cells was...
The proapoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptors death receptor (DR) 4 and DR5 are attractive targets to develop the receptor-specific agonistic monoclonal antibodies (mAb) as anticancer agents because of their tumor-selective cell death-inducing activity. Here, we report a novel mAb, AY4, raised against human DR4 in mice. ELISA analysis revealed that AY4 specifically bound without competition with TRAIL for binding. Despite distinct binding regions...
Preconditioning nerve injury enhances axonal regeneration of dorsal root ganglia (DRG) neurons in part by driving pro-regenerative perineuronal macrophage activation.How these macrophages influence the neuronal capacity axon remains elusive.We report that oncomodulin (ONCM) is produced from regeneration-associated and strongly influences DRG sensory axons.We also attempted to promote nanogel-mediated delivery ONCM DRGs.Methods: In vitro neuron-macrophage interaction model preconditioning...
Targeting particular mRNAs for degradation is a fascinating approach to achieve gene silencing. Here we describe new silencing tool exploiting cell-penetrating, nucleic-acid hydrolyzing, single-domain antibody of the light-chain variable domain, 3D8 VL. We generated synthetic library VL on yeast surface by randomizing residues located in one two beta-sheets. Using 18-bp single-stranded nucleic acids as target substrates, including human Her2/neu-targeting sequence, selected variants that had...
More than 50% of the worldwide effective radiation dose from natural sources is known to be due 222 Rn. Indoor radon levels were measured in homes with long-term exposure and relation time spent for residents. Measurements conducted after categorizing by type, results (mean ± S.D) showed that detached houses, apartments multifamily houses had concentrations 97 110, 51 24 59 34 Bq/m 3 , respectively. Surveys 620 people analyse resident awareness residents spend homes. Using these results,...
A well-characterized recombinant anti-idiotype to an anti-DNA antibody can be useful for studies of the regulation anti-DNA-producing B cells. Using a hybridoma technique, monoclonal anti-idiotypic antibody, designated O2F3, was obtained, and its scFv gene constructed. O2F3 single chain Fv (scFv) produced against idiotope 3D8, that obtained from autoimmune-prone mouse, MRL-lpr/lpr. Here we describe production in vitro characterization scFv, compare it with parent IgM. To characterize IgM,...