A5055862551- Human-Animal Interaction Studies
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Enzyme Structure and Function
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Cancer-related Molecular Pathways
- Neuroendocrine regulation and behavior
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Mitochondrial Function and Pathology
- Diabetes Treatment and Management
- Salmonella and Campylobacter epidemiology
- Dental Implant Techniques and Outcomes
- Glycogen Storage Diseases and Myoclonus
- Nuclear Structure and Function
- Racial and Ethnic Identity Research
- Cancer, Stress, Anesthesia, and Immune Response
- Autophagy in Disease and Therapy
- Steroid Chemistry and Biochemistry
Seoul National University
2018-2024
Korea Institute of Brain Science
2019-2022
Korea Institute of Science and Technology
2019-2022
Korean Association Of Science and Technology Studies
2022
Genexine (South Korea)
2020
CHA University
2011-2018
Pusan National University Hospital
2017
Pusan National University
2013-2016
Chonnam National University Hwasun Hospital
2015
Genome-wide next-generation sequencing has revealed several driver mutations and allowed the establishment of a molecular taxonomy gastric cancer. However, there are few detailed studies on mutational spectrum poorly cohesive carcinoma. Thus, this study aim to investigate its mutation profile based clinicopathological characteristics.Herein, we analysed pattern 77 genes in cohort 91 patients with carcinoma by using targeted sequencing, evaluated significance various histological pattern,...
Abstract Background DNA methylation is an epigenetic regulatory mechanism that plays essential role in mediating biological processes and determining phenotypic plasticity organisms. Although the horse reference genome whole transcriptome data are publically available global yet to be known. Results We report first genome-wide characteristics from skeletal muscle, heart, lung, cerebrum tissues of thoroughbred (TH) Jeju (JH) horses, indigenous Korea breed, respectively by methyl-DNA...
Abstract Bacterial cell division is a fundamental process that results in the physical separation of mother into two daughter cells and involves set proteins known as divisome. Among them, FtsQ/FtsB/FtsL complex was scaffold protein complex, but its overall structure exact function not precisely known. In this study, we have determined crystal periplasmic domain FtsQ with C-terminal fragment FtsB, showed region FtsB key binding via mutational analysis vitro vivo . We also obtained solution...
Athletic performance is an important criteria used for the selection of superior horses. However, little known about exercise-related epigenetic processes in horse. DNA methylation a key mechanism regulating gene expression response to environmental changes. We carried out comparative genomic analysis genome-wide profiles blood samples two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions...
The balance between major DNA double-strand break (DSB) repair pathways is influenced by binding of the Ku complex, a XRCC5/6 heterodimer, to DSB ends, initiating non-homologous end joining (NHEJ) but preventing additional resection and homologous recombination (HR). However, key molecular cue for recruitment sites unknown. Here, we report that FOXL2, forkhead family transcriptional factor, directs pathway choice acetylation-dependent Ku. Upon induction, SIRT1 translocates nucleus...
Rising antibiotic resistance urgently begs for novel targets and strategies discovery. Here, we report that over-activation of the periplasmic DegP protease, a member highly conserved HtrA family, can be viable strategy development. We demonstrate tripodal peptidyl compounds mimic DegP-activating lipoprotein variants allosterically activate inhibit growth an Escherichia coli strain with permeable outer membrane in DegP-dependent fashion. Interestingly, these bacterial at temperature which is...
The transcription factor TWIST has been reported to play an important role in tumor progression as well resistance anti-cancer drugs. However, the of gastric cancer and molecular mechanisms by which this protein elicits drug remain poorly understood. We transfected cell lines with lentiviral vector generate TWIST-overexpressing stable lines. Our study showed that overexpression not only increased migration invasion but also induced paclitaxel cancer. Paclitaxel death dose-dependent manner;...
Many psychiatric disorders accompany deficits in cognitive functions and synaptic plasticity, abnormal lipid modifications of neuronal proteins are associated with their pathophysiology. Lipid modifications, including palmitoylation myristoylation, play crucial roles the subcellular localization trafficking proteins. Cyclin Y (CCNY), enriched postsynaptic compartment, acts as an inhibitory modulator functional structural long-term potentiation (LTP) hippocampal neurons. However, cellular...
E3 ubiquitin ligase Mdm2 facilitates β-arrestin ubiquitination, leading to the internalization of G protein–coupled receptors (GPCRs). In this process, β-arrestins bind and recruit it receptor; however, molecular architecture β-arrestin-Mdm2 complex has not been elucidated yet. Here, we identified β-arrestin-binding region (ABR) on solved crystal structure β-arrestin1 in with ABR peptide. The acidic residues positively charged concave side N-domain. C-tail is still bound N-domain, indicating...
The N-Myc downstream-regulated gene (NDRG) family belongs to the α/β-hydrolase fold and is known exert various physiologic functions in cell proliferation, differentiation, hypoxia-induced cancer metabolism. In particular, NDRG3 closely related proliferation migration of prostate cells, recent studies reported its implication lactate-triggered hypoxia responses or tumorigenesis. However, underlying mechanism for remains unclear. Here, we report crystal structure human at 2.2 Å resolution,...
Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed impressive potential to slow age-associated degradation neuronal and cognitive functions. Translating such findings clinical realities, however, requires effective ways for assessing treatment efficacy; ideal methods should be minimally invasive, amenable serial assays, cost-effective, quantitative.
Prevalent in diverse protein interactomes, intrinsically disordered proteins or regions (IDPs IDRs) often drive assembly of higher-order macromolecular complexes, using multiple target-binding motifs. Such IDP hubs are suggested to process various cellular signals and coordinate relevant biological processes. However, the mechanism functional regulation remains elusive due challenges dissecting their intricate protein–protein interaction networks. Here we present basic thermodynamic models...
Abstract Although both the p53 and forkhead box (FOX) family proteins are key transcription factors associated with cancer progression, their direct relationship is unknown. Here, we found that FOX bind to non-canonical homotypic cluster of promoter region (TP53). Analysis crystal structures (FOXL2 FOXA1) bound indicated they interact a 2:1 stoichiometry accommodated by FOX-induced DNA allostery. In particular, exhibited distinct dimerization patterns in recognition same p53-DNA; dimer...
Cyclin Y (CCNY) is a member of cyclin superfamily proteins involved in the regulation cell cycle proliferating cells. Intriguingly, CCNY highly expressed terminally differentiated neuronal cells multiple brain regions and acts as postsynaptic protein, which plays an inhibitory role long-term potentiation. However, pathophysiological significance nervous system remains largely unexplored. In this study, we revisited our RNA-sequencing (RNA-seq) data obtained from cultured hippocampal neurons...
While positive regulators of hippocampal long-term potentiation (LTP) have extensively been investigated, relatively little is known about the inhibitory LTP. We previously reported that Cyclin Y (CCNY), a member cyclin family generally to function in proliferating cells, novel postsynaptic protein serves as negative regulator functional However, whether CCNY plays role structural LTP, which mechanistically linked and mechanisms are involved CCNY-mediated suppression LTP at molecular level...
Spatial learning and memory flexibility are known to require long-term potentiation (LTP) depression (LTD), respectively, on a cellular basis. We previously showed that cyclin Y (CCNY), synapse-remodeling cyclin, is novel actin-binding protein an inhibitory regulator of functional structural LTP in vitro. In this study, we report Ccny knockout (KO) mice exhibit enhanced weak LTD at Schaffer collateral-CA1 synapses the hippocampus. accordance with LTP, KO improved spatial memory. However,...
To evaluate the pharmacokinetic and pharmacodynamic properties of a novel glycosylated Fc-fused glucagon-like peptide-1(GLP-1-gFc) receptor agonist with distinctive binding affinity, designed to improve in vivo stability safety relative commercial GLP-1 analogue dulaglutide, assess its profile pharmacokinetics healthy humans.We constructed GLP-1-gFc determined affinity potency using vitro instrumental cell-based analyses followed by comparison glucose-lowering gastrointestinal side effects...