A5033237694- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Mechanisms of cancer metastasis
- Lung Cancer Research Studies
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Cancer therapeutics and mechanisms
- Cytokine Signaling Pathways and Interactions
- Cancer Genomics and Diagnostics
- Cancer Mechanisms and Therapy
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Chronic Myeloid Leukemia Treatments
- Quinazolinone synthesis and applications
- Pancreatic and Hepatic Oncology Research
- Neuroscience and Neuropharmacology Research
- NF-κB Signaling Pathways
- Helicobacter pylori-related gastroenterology studies
- TGF-β signaling in diseases
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
Korea Research Institute of Bioscience and Biotechnology
2015-2024
Korea University of Science and Technology
2015-2024
Itami City Hospital
2019-2020
Yonsei University
1998-2018
Severance Hospital
2018
United States Army Institute of Surgical Research
2015-2017
Armed Forces Capital Hospital
2017
Genome Research Foundation
2015
Korea University
2012
Chungnam National University Hospital
2012
Abstract Intracellular Vitamin C (VC) is maintained at high levels in the developing brain by activity of sodium-dependent VC transporter 2 (Svct2), suggesting specific functions development. A role as a cofactor for Fe(II)-2-oxoglutarate-dependent dioxygenases has recently been suggested. We show that supplementation neural stem cell cultures derived from embryonic midbrains greatly enhanced differentiation toward midbrain-type dopamine (mDA) neurons, neuronal subtype associated with...
Histone-modifying enzymes play a pivotal role in gene expression and repression. In human, DOT1L (Dot1-like) is the only known histone H3 lysine 79 methyltransferase. hDOT1L associated with transcriptional activation, but general mechanism connecting to active transcription remains largely unknown. Here, we report that interacts phosphorylated C-terminal domain of actively transcribing RNA polymerase II (RNAPII) through region conserved uniquely multicellular DOT1 proteins. Genome-wide...
AbstractMicroRNAs act as negative regulators of gene expression, and the altered expression microRNAs by epigenetic mechanisms is strongly implicated in carcinogenesis. Here we report that microRNA-10b (miR-10b) was silenced gastric cancer cells promoter methylation. In this study, using a methylation array bisulfate pyrosequencing analysis, found miR-10b CpGs were heavily methylated cancers. Clinicopathologic data showed increased with patient age occurred significantly more frequently...
DNA methylation and hydroxymethylation have been implicated in normal development differentiation, but our knowledge is limited about the genome-wide distribution of 5-methylcytosine (5 mC) 5-hydroxymethylcytosine hmC) during cellular differentiation. Using an vitro model system gradual differentiation human embryonic stem (hES) cells into ventral midbrain-type neural precursor terminally dopamine neurons, we observed dramatic changes 5 mC hmC patterns lineage commitment. The pattern was...
Abstract Homeostasis of neutrophils—the blood cells that respond first to infection and tissue injury—is critical for the regulation immune responses regulated through granulopoiesis, a multi-stage process by which neutrophils differentiate from hematopoietic stem cells. Granulopoiesis is highly dynamic altered in certain clinical conditions, such as pathologic iatrogenic neutropenia, described demand-adapted granulopoiesis. The granulopoiesis under stress not completely understood because...
The dorsal and ventral regions of the hippocampus are important in cognitive emotional processing, respectively. Various approaches have revealed differential molecular structural characteristics, functional roles hippocampus. Recent RNA sequencing (RNA-seq) technology has enriched our understanding by elucidating more detailed information on gene expression patterns. However, no RNA-seqbased study profiles developing been reported. Using bioinformatic analysis conjunction with quantitative...
H2B monoubiquitylation (H2Bub1), which is required for multiple methylations of both H3K4 and H3K79, has been implicated in gene expression numerous organisms ranging from yeast to human. However, the molecular crosstalk between H2Bub1 other modifications, especially remains unclear vertebrates. To better understand functional role H2Bub1, we measured genome-wide histone modification patterns human cells. Our results suggest that dual roles, one H3 methylation dependent, another independent....
The spatiotemporal regulation of immune cells in lymph nodes (LNs) is crucial for mounting protective T-cell responses, which are orchestrated by dendritic (DCs). However, it unclear how the DC subsets altered inflammatory milieu LNs. Here, we show that inflamed LNs Listeria-infected mice characterized clustering neutrophils and monocytes IFN-γ production. Significantly, early responses coupled with differentiation not one, but two types CD64+CD11c+MHCII+ DCs. Through assessment chemokine...
Anaplastic lymphoma kinase (ALK) inhibitors are highly effective in patients with ALK fusion-positive lung cancer, but acquired resistance invariably emerges. Identification of secondary mutations has received considerable attention, most cases cannot be explained by genetic causes alone, raising the possibility epigenetic mechanisms drug resistance. Here, we investigated dynamic changes transcriptome and enhancer landscape during development to inhibitors. Histone H3 lysine 27 acetylation...
The Popeye domain-containing ( POPDC ) genes BVES , POPDC2 and POPDC3 encode proteins that regulate cell–cell adhesion cell migration during development. Herein, we report the frequent downregulation of by promoter hypermethylation in gastric cancer. expression 11 cancer lines 96 paired tumor normal adjacent tissues was analyzed with quantitative reverse transcription–polymerase chain reaction. methylation status methylated DNA immunoprecipitation sequencing, bisulfite sequencing...
Abstract Leucine-rich repeat-containing 3B (LRRC3B) is an evolutionarily highly conserved leucine-rich protein, but its biological significance unknown. Using restriction landmark genomic scanning and pyrosequencing, we found that the promoter region of LRRC3B was aberrantly methylated in gastric cancer. Gastric cancer cell lines displayed epigenetic silencing LRRC3B, treatment with DNA methylation inhibitor 5-aza-2′-deoxycytidine and/or histone deacetylase trichostatin A increased...
Abstract The promoter region of Discoidin, CUB and LCCL domain containing 2 (DCBLD2) was found to be aberrantly methylated in gastric cancer cell lines primary cancers, as determined by restriction landmark genomic scanning. DCBLD2 expression inversely correlated with methylation lines. Treatment 5-aza-2′-deoxycytidine trichostatin A partially reversed restored gene DCBLD2-silenced In an independent series 82 paired cancers adjacent normal tissues, down-regulated 79% compared tissues...
In this study, the promoter of gene coiled-coil domain-containing 67 ( CCDC67 ) was found to be frequently methylated in gastric cancer cell lines and primary tumors, as examined by restriction landmark genomic scanning. addition, expression down-regulated 72.7% tested. most cases, down-regulation associated with CpG hypermethylation promoter. Treatment 5-aza-2ʹ-deoxycytidine and/or trichostatin A restored lines. Pyrosequencing analysis 150 paired samples revealed that methylation increased...
Recent evidence has shown that the level of 5-hydroxymethylcytosine (5 hmC) in chromosomal DNA is aberrantly decreased a variety cancers, but whether this decrease cause or consequence tumorigenesis unclear. Here we show that, gastric 5 hmC correlates with ten-eleven translocation 1 (TET1) expression, which strongly associated metastasis and poor survival patients cancer. In cancer cells, TET1-targeted siRNA induced hmC, whereas TET1 overexpression an increase reduced cell proliferation,...
Loss of functional β-cell mass is an essential feature type 2 diabetes, and maintaining mature identity important for preserving a mass. However, it unclear how β-cells achieve maintain their identity. Here we demonstrate novel function protein arginine methyltransferase 1 (PRMT1) in Prmt1 knockout fetal adult induced which was aggravated by high-fat diet–induced metabolic stress. Deletion resulted the immediate loss histone H4 3 asymmetric dimethylation (H4R3me2a) subsequent The expression...