A5101418166- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Chemokine receptors and signaling
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Pain Mechanisms and Treatments
- Antimicrobial Peptides and Activities
- RNA modifications and cancer
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Pharmacological Effects of Natural Compounds
- Immune cells in cancer
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Cancer, Stress, Anesthesia, and Immune Response
- Electrolyte and hormonal disorders
- Carbohydrate Chemistry and Synthesis
- Thermal Regulation in Medicine
- Acute Kidney Injury Research
- Cardiac electrophysiology and arrhythmias
- Solar-Powered Water Purification Methods
- Macrophage Migration Inhibitory Factor
Konkuk University
2015-2024
Gachon University
2023-2024
Korea Research Institute of Bioscience and Biotechnology
2015-2023
Korea University of Science and Technology
2015-2020
Konkuk University Medical Center
2018
Abstract Editor’s Perspective What We Already Know about This Topic Article Tells Us That Is New Background Clusters of differentiation 39 and 73, enzymes expressed on the surface regulatory T cells, promote cancer recurrence metastasis by suppressing immune cells. The authors hypothesized that propofol is less immunosuppressive than volatile anesthetics. objective this randomized trial was to compare changes in cluster 73 expression cells between propofol- sevoflurane-based anesthesia...
The rupture of an atherosclerotic plaque is one the main causes coronary artery thrombotic occlusion, leading to myocardial infarction. However, exact mechanism and causal risk factors for remain unclear. To identify a potential molecule that can influence rupture, we investigated protein expression in serum from patients with acute infarction (AMI) stable angina (SA), using proteomic analysis. six proteins, including fibrinogen, fetuin-B, keratin 9, proapolipoprotein were altered AMI...
Many studies have focused on global hypomethylation or hypermethylation of tumor suppressor genes, but less is known about the impact promoter oncogenes. We previously showed that methylation may gradually increase decrease during transition from gastric mucosa (GM) to intestinal metaplasia (IM) cancer (GC). In our study, we regional CpG promoter-proximal DNA transcription factor ONECUT2 (OC2) in IM and GC cells. validated OC2 160 primary GCs, which level correlated negatively with mRNA...
Recent evidence has shown that the level of 5-hydroxymethylcytosine (5 hmC) in chromosomal DNA is aberrantly decreased a variety cancers, but whether this decrease cause or consequence tumorigenesis unclear. Here we show that, gastric 5 hmC correlates with ten-eleven translocation 1 (TET1) expression, which strongly associated metastasis and poor survival patients cancer. In cancer cells, TET1-targeted siRNA induced hmC, whereas TET1 overexpression an increase reduced cell proliferation,...
Loss of functional β-cell mass is an essential feature type 2 diabetes, and maintaining mature identity important for preserving a mass. However, it unclear how β-cells achieve maintain their identity. Here we demonstrate novel function protein arginine methyltransferase 1 (PRMT1) in Prmt1 knockout fetal adult induced which was aggravated by high-fat diet–induced metabolic stress. Deletion resulted the immediate loss histone H4 3 asymmetric dimethylation (H4R3me2a) subsequent The expression...
Hypophosphatemia is common during continuous renal replacement therapy (CRRT) in critically ill patients and can cause generalized muscle weakness, prolonged respiratory failure, myocardial dysfunction. This study aimed to investigate the efficacy safety of adding phosphate dialysate solutions treat hypophosphatemia occurring intensive CRRT patients.We retrospectively analyzed 73 treated with (effluent flow ≥35 ml/kg/hr) care unit. The control group (group 1, n = 22) received no...
Extracellular vesicles (EVs) are nanovesicles of endocytic origin released by cells and found in human bodily fluids. EVs contain both mRNA microRNA (miRNA), which can be shuttled between cells, indicating their role cell communication. This study investigated whether nasal secretions these RNA. were isolated from lavage fluid (NLF) using sequential centrifugation. characterized EV sizes identified transmission electron microscopy (TEM). In addition, miRNA expression was different the...
This study aimed to investigate the effects of ketamine, an N-methyl-D-aspartate (NMDA) receptor antagonist, on endoplasmic reticulum (ER) stress in rats with neuropathic pain (NP). NP was induced through ligation and transection sciatic nerve. After confirmation NP, animals were randomly divided into ketamine control groups. The group administered 50 mg/kg at 15, 18, 21 days after surgery. expression NMDA subtype 2B (NR2B) ER markers spinal cord (L5) evaluated. ipsilateral side surgery less...
This experimental study was designed to evaluate the effect of ulinastatin, a urinary trypsin inhibitor, on postoperative cognitive dysfunction (POCD) in rats under general anesthesia with isoflurane, aspect behavior, as evaluated using Y-maze test and focusing microglial activity. Ulinastatin (50,000 U/mL) normal saline (1 mL) were randomly (1:1) administered intraperitoneally ulinastatin control groups, respectively, before anesthesia. Anesthesia isoflurane 1.5 volume% maintained for 2 h....
Epigenetic dysregulation is a major driver of tumorigenesis. To identify tumor-suppressive microRNAs repressed by DNA methylation in gastric cancer (GC), we analyzed the genome-wide and microRNA expression profiles EpCAM+/CD44+ GC cells. Among set screened, miR-1271 was identified as GC. Forced substantially suppressed growth, migration, invasion candidate target genes signaling pathways regulated miR-1271, performed RNA sequencing. down-regulated MAP2K1 (MEK1) significantly associated...
Cell-penetrating peptides (CPPs), a group of small capable promoting the transport molecular cargo across plasma membrane, have become important tools in cellular uptake exogenously delivered macromolecules. GV1001, peptide derived from reverse-transcriptase subunit telomerase (hTERT) and developed as vaccine against various cancers, reportedly has unexpected CPP properties. Unlike typical CPPs, such HIV-1 TAT peptide, GV1001 enabled cytosolic delivery macromolecules proteins, DNA siRNA via...
Background: This study investigated the effects of glycyrrhizin (GR) on ratio myeloid-derived suppressor cells (MDSCs) to cluster differentiation (CD)11b+Gr1 myeloid in heart and lungs lipopolysaccharide (LPS)-induced septic mice. Methods: Mice were divided into three groups: Control, LPS, LPS+GR. After intraperitoneal injection phosphate-buffered saline for Control group, LPS a combination GR LPS+GR fluorescence-activated cell sorting was utilized evaluate cytokines immune blood, heart,...
We hypothesised that the blood levels of immune cells would be related to progression colorectal cancer and regional lymph node metastasis. investigated association between metastasis in patients. Patients with American Joint Committee on Cancer (AJCC) stages 1 2 were assigned Early stage group those AJCC 3 4 Late group. Blood circulating cells, such as cluster differentiation (CD)4+ including T helper (Th1) 17 (Th17) regulatory (Treg) CD8+ natural killer (NK) assessed using...
Myeloid-derived suppressor cells (MDSCs) are highly immunosuppressive myeloid that show increased expression in cancer patients; however, the molecular mechanisms underlying their generation and function unclear.Whereas granulocytic-MDSCs correlate with poor overall survival breast (BC), presence relevance of monocytic (Mo)-MDSCs unknown.Here, we report for first time chemokine receptor production by Mo-MDSCs BC patients.A clear population typical cell surface phenotype (human leukocyte...
Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia. Methods: Rats were randomly divided into Propofol Isoflurane groups. Anaesthesia was maintained with for group or during 3 h. ER from lymphocytes blood tissues evaluated between two groups after euthanasia. Reactive oxygen species (ROS) tissues, cytokines also checked. An immunohistochemical assay marker performed. Results: After...
Background: This study was designed to investigate the effect of cluster differentiation (CD)39 and CD73 inhibitors on expresion tumour-associated macrophages (TAMs), M1- versus M2-tumour phenotypes in mice with colon cancer. Methods: An vivo co-culture cancer cells immune from bone marrow (BM) performed. After confirmation polyoxotungstate (POM-1) as an inhibitor CD39 TAMs, were randomly divided into a control group without POM-1 POM-1, respectively, after subcutaneous injection CT26 cells....
Background: The study examined the difference in expression of receptor for activated C kinase 1 (RACK1) between anaesthesia with propofol and isoflurane rats myocardial ischemia-reperfusion injury (IRI). Methods: Male Sprague-Dawley were studied. Anaesthesia was induced xylazine 20 µg/g by intraperitoneal injection maintained or isoflurane. Myocardial IRI ligating left anterior descending artery hour. Reactive oxygen species (ROS), cardiomyocyte apoptosis, RACK1 toll-like 4 (TLR4), heart...
Pathological changes in the epigenetic landscape of chromatin are hallmarks cancer. The caudal-type homeobox gene CDX2 is not expressed normal gastric epithelia but rather adult intestinal epithelia, and it overexpressed metaplasia (IM). However, remains unclear how transcription suppressed epithelial cells IM. Here, we demonstrate that methylation promoter increases with age Helicobacter pylori-positive, noncancerous tissue, whereas demethylated paired tumors which upregulated. Moreover,...
Background: We hypothesized that endoplasmic reticulum stress (ER) would be associated with exosomes in ventilator-induced lung injury (VILI), and inhibition of ER linked to less occurrence VILI or damage VILI.
The current production of the Japanese encephalitis virus (JEV) vaccine is based on animal cells, where various risk factors for human health should be resolved. This study used a transient expression system to express chimeric protein composed antigenic epitopes from JEV envelope (E) in Nicotiana benthamiana. multi-epitope peptide (MEP) sequences fused with FLAG-tag or 6× His-tag at C- N-terminus purification were introduced into plant vectors and expression. Among constructs, vector...
Coronavirus disease 2019 (COVID-19) is known to have a high incidence of loss smell and taste. However, studies in the early stages COVID-19 pandemic evaluated these symptoms using subjective surveys simple olfactory tests only. Hence, we compared gustatory characteristics patient groups with dysfunction (C19OD) non-COVID-19 postinfectious (PIOD) an objective test significance training both groups.We retrospectively analyzed medical records 14 patients decreased sense after having positive...
This study investigated the association between different ratios of balanced salt based-crystalloid (PLASMA SOLUTION-A [CJ HealthCare, Seoul, Korea]) (the crystalloid for blood loss, 1:1, 1:2 and 1:3) or salt-based colloid (VOLULYTE 6% [Fresenius Kabi, Germany]) ratio 1:1) to restore loss immune response in rats with haemorrhagic shock. About 50% total estimated volume was removed after anaesthesia. The fluid administered resuscitation exsanguination, according type exsanguinated...